Carbohydrate Utilization & O/F Tests

The degradation of which carbohydrate is most important?

Lactose

Bacteria DIFFER based on their ability to use CARBOHYDRATES

What does Permease do in Lactose Utilization?

BRING LACTOSE INTO the bacteria

What does Beta-galactosidase do in Lactose Utilization?

SPLIT THE LACTOSE

What is lactose split into?

Glucose and Galactose

What are the 2 enzymes involved in Lactose Utilization?

Permease and Beta-galactosidase

A deficiency/absence of which enzyme can cause DELAYED lactose utilization?

Permease

In the degradation of Glucose, all paths will produce which FINAL carbon product?

Pyruvate

EMP = 2 pyruvate

EDP = 1 Pyruvate

What else can the degradation of glucose result in?

ATP production

What are Oxidation-Fermentation (O/F) Tests used to do?

How a bacterium uses a carbohydrate

Oxidatively or fermentatively

What other condition is tested in the O/F Tests?

Aerobic or anaerobic

In the O/F tests, which type of carbohydrate use will REQUIRE OXYGEN for acid prodcution?

Oxidative

In the O/F tests, which type of carbohydrate use will NOT REQUIRE oxygen for acid production?

Fermentative

Oxidative use of carbohydrates can be detected under what conditions?

Aerobic

Fermentative use of carbohydrates can be detected under what conditions?

Anaerobic

What 3 other products can be seen in the fermentative use of carbohydrates?

GAS

Alcohol

Acid

Low peptone concentration in the O/F Test Basal Media allows for detection of what?

Detection of SMALL AMOUNTS of acid

In the O/F Basal Media What is the NORMAL color of the media?

Green

In the presence of Acidic pH, what is the color of the O/F media?

YELLOW = acid will always be YELLOW COLOR

In the presence of ALKALINE pH, what is the color of the O/F media?

BLUE

In the O/F tests, an open tube is associated with what oxygen condition?

Aerobic

In the O/F tests, a tube OVERLAYED WITH OIL is associated with what oxygen conditions?

Anaerobic

What type of Carbohydrate utilization is seen in the image?

Oxidative ACID PRODUCTION (yellow)

REQUIRED OXYGEN

What type of Carbohydrate utilization is seen in the image?

Fermentative ACID PRODUCTION (yellow)

Anaerobic/not requiring oxygen

What type of Carbohydrate utilization is seen in the image?

NO CARBOHYDRATE UTILIZATION

ABSENCE OF YELLOW / acid production indicates no carb utilization