DNA polymerase and DNA ligase

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Last updated 1:12 PM on 6/15/26

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DNA polymerase

used to convert single strand cDNA to double stranded DNA

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DNA polymerase construction

second strand is built by pairing free nucleotides with the complementary bases on cDNA

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DNA ligase

catalyses the formation of phosphodiester bonds in the sugar phosphate backbone

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DNA ligase enables

isolated desired gene to b spliced into a vector to transferred to a new organism