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magnification
how much larger the specimen appears
resolution
ability to distinguish two close points as separate
total magnification
ocular lens x objective lens
scanning lens
4x lens
low power lens
10x lens
high dry lens
40x lens
oil immersion lens
100x
improves resolution by reducing light refraction
streak plate
isolates individual colonies
loop dragged across agar plate
each section spreads fewer cells (forms isolated colonies)
*diluting bacteria across surface
good streak plate
heavy growth at beginning
less growth farther
isolated colonies near final
simple staining
uses one dye to see cell shape, size, arrangement
does not differentiate organisms into major groups like Gram stain does
culture media
nutrient material used to grow microorganisms
broth
liquid medium
agar
solidifying agent used in many solid media
agar plate
petri dish containing solid medium
agar slant
agar solidified at an angle in a tube
agar deep
agar solidified upright in a tube
general purpose media
supports growth of many organisms
selective media
favors growth of certain organisms while inhibiting others
pure culture
one type of microorganism
coccus
sphere
bacillus
rod
spirillum
spiral
staphylo
clusters
strepto
chains
gram-positive
purple/blue
gram-negative
pink/red
gram stain order
crystal violet
iodine
alcohol
safranin