Lab Quiz Prep

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Last updated 6:34 AM on 2/27/25
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22 Terms

1
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Catalase test
A test that determines the presence of the enzyme catalase, indicated by rapid bubbling due to the production of O2 gas when hydrogen peroxide is added.
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Positive catalase test
Indicated by rapid bubbling when hydrogen peroxide is added.
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Negative catalase test
Indicated by no bubbling when hydrogen peroxide is added.
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Citrate utilization test
A test using Simmons citrate agar to check if an organism can use citrate as a sole carbon source, indicated by a deep blue color.
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Positive citrate test
Bacterial growth accompanied by a deep blue color in the medium.
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Negative citrate test
Little to no growth with no change in the color of the medium.
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Motility test
A test that assesses bacterial motility through growth patterns in motility agar.
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Positive motility test
Indicated by red, diffuse growth radiating from the stab line.
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Negative motility test
Indicated by red growth only along the stab line.
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Triple Sugar Iron Agar (TSIA)
A differential medium that differentiates organisms based on their ability to reduce sulfur and ferment carbohydrates.
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Yellow slant/Yellow butt
Indicates fermentation of glucose and lactose/sucrose with no sulfur reduction.
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Yellow slant/Black butt
Indicates fermentation of glucose and lactose/sucrose with sulfur reduction.
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Red slant/Yellow butt
Indicates fermentation of glucose and peptone with no sulfur reduction.
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Red slant/Black butt
Indicates fermentation of glucose and peptone with sulfur reduction.
15
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Carbohydrate fermentation test
A test that determines an organism's ability to ferment carbohydrates using phenol red broth.
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Positive carbohydrate fermentation test
Indicated by a yellow color change in the medium due to acid production and potentially gas trapped in an inverted Durham tube.
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Urease detection test
A test measuring the degradation of urea to ammonia, indicated by a color change to bright pink.
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Positive urease test
Indicated by a pink color in the medium due to alkaline conditions.
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Methyl red test
A differential test using methyl red as a pH indicator to identify mixed acid fermenters.
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Positive methyl red test
Indicated by a red color in the broth, indicating acidic end products.
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Nitrate reduction test
A test that determines the ability of an organism to reduce nitrate to nitrite, indicated by the formation of a red compound after reaction.
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Positive nitrate reduction test
Indicated by the formation of a red-colored compound after adding reagents.