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infection control and disinfection
What is Bioburden?
Blood, saliva, and bodily fluids
What is a primary source of cross-contamination among staff?
When dental staff touch surfaces with contaminated gloves.
A surface in contact with blood, saliva, or other infectious material contains live microorganisms. True or False?
True
What is Contamination?
Objects, barriers or surfaces that have come into contact with microorganisms either directly or indirectly
What is Biofilm?
Consists of bacterial cells and other microbes that adhere to surfaces and form a protective slime layer
Why do we flush our dental units?
Biofilm is a type of plaque that can cause an infection of the water delivery system
may contain bacteria, fungi, algae, and protozoa
bacteria in biofilm (slime layer) is hard to remove
What are Critical Instruments?
Items used to penetrate soft tissue or contact bone (not including enamel)
→ ANYTHING THAT GOES SUBGINGIVAL!
greatest risk of transmitting infection
needs to be sterilized by heat
examples: forcepts, scalpels
What are Semi-Critical Instruments?
Things that contact within the mouth, but not subgingival
lower risk of transmission
should be sterilized/high-levelled disfinfected
examples: HVE tips, amalgam carriers, mouth mirror
What are Non-Critical Instruments?
Contact only the skin, no exposure to blood, blood contaminated saliva or tissues
least risk of transmission
cleaned & processed with intermediate/low level disinfectant each use
examples: lead apron, blood pressure cuff
What is Sanitation? (Cleaning)
Removes visible debris, blood, and saliva from an instrument or surface
Reduces the number of microorganisms and bioburden
Done before sterilization and disinfection

What is Disinfection?
The destruction of some, but not all (disease-causing) micro-organisms after cleaning/sanitation
Spores are killed during disinfection procedures. True or False?
False, spores still remain

What is Sterilization?
The destruction of all life forms including bacterial spores, viruses, bacteria & fungi
***cannot occur through attached debris or blood***
There is such thing as partial or extra sterilizing. True or False?
False. Sterilization is a definite state: Sterile or not sterile.
What is Precleaning?
To clean before disinfecting
What can be used for cleaning?
Soap and water or a cleaner-disinfectant
Why must surfaces be cleaned even without visible blood?
Even a thin layer of saliva can reduce disinfectant effectiveness
What does Precleaning remove?
Bioburden
What type of disinfectant must dental offices use?
EPA-approved hospital-level disinfectant
What does high-level disinfection destroy?
Kills some, but not all bacterial endospores
→ (strongest)
What is high-level disinfection used for?
Disinfecting semicritical items that cannot tolerate heat sterilization
Does high-level disinfection inactivate TB? Yes or No?
Yes
What does intermediate-level disinfection kill?
TB and less resistant microorganisms such as bacteria, viruses, and fungi
→ (2nd strongest)
Does intermediate-level disinfection inactivate TB?
Yes
What is intermediate disinfection used for?
Disinfecting non-critical items or surfaces that have been contaminated with blood or saliva
Where are intermediate disinfections commonly used?
Dental operatory surfaces
When is low-level disinfection used?
On surfaces NOT contaminated with blood
Does low-level disinfection kill TB?
No
Where is low-level disinfection used?
Dental office for general housekeeping (floors and walls)
What does non-tuberculocidal mean?
Does not kill TB
How can instruments be manually cleaned?
Scrubbing with a stiff-bristled brush under running water
What machines are commonly used to clean instruments?
Ultrasonic cleaners or automated washers
When must instruments be cleaned?
Before sterilization
How long must Optim 33 stay wet to kill TB?
Must remain wet for 1 minute
How long must disinfectant stay on surfaces?
Follow the manufacturer's instructions
Identify Qualities of an Ideal Surface Disinfectant
**Must kill TB and viruses (be virucidal)
Registered with EPA (environmental protection agency)
Have DIN (drug identification number)
Rapidly kill bacteria
Have residual activity
Minimal toxicity
Discuss Advantages and Disadvantages of Iodophors
Advantages:
Effective in 5–10 minutes.
Intermediate-level disinfectant EPA registered
Disadvantages:
Can corrode or stain surfaces due to Iodine
May irritate tissues
Hard water makes them ineffective.
Discuss Advantages and Disadvantages of Synthetic Phenol Compounds
Advantages:
EPA registered intermediate-level disinfectant
Broad spectrum
Disadvantages:
Leaves a film/residue on surfaces.
Some must be prepared daily.
Discuss Advantages and Disadvantages of Sodium Hypochlorite
Advantages:
Fast-acting.
Inexpensive.
Broad-spectrum.
Intermediate-level
Disadvantages:
Needs daily preparation.
Strong smell and corrosive
Irritates skin and eyes.
No longer recommended or EPA-approved
Discuss Disadvantages of Alcohol
Disadvantages:
Not effective when blood, saliva or debris are present.
^^Evaporates too quickly to disinfect properly.
Not recommended for surface disinfection.
Discuss Advantages and Disadvantages of Quaternary Ammonia (like lysol)
Advantages:
Broad-spectrum.
Low toxicity.
Effective in 6–10 minutes.
Disadvantages:
Inactivated by organic matter.
Inactivated by certain detergents.
Can damage some materials.
Discuss Advantages and Disadvantages of Chlorine Dioxide
Advantages:
High-level disinfectant
Fast-acting surface disinfectant (3 minutes).
Can act as a chemical sterilant (6 hours).
Disadvantages:
Does not penetrate debris easily
Corrosive to aluminum containers
Discuss Advantages and Disadvantages of Glutaraldehyde
Advantages:
High-level disinfectant.
Can sterilize.
Used for plastic items that cannot tolerate heat sterilization.
Disadvantages:
Very toxic.
NOT APPROVED FOR STERILIZATION IN DENTAL OFFICES IN CANADA
Discuss Advantages of Accelerated hydrogen peroxide (AHP) aka OPTIM
Advantages:
Tuberculocidal
Effective in 1 minute
Kills many variety of microorganisms
What are the three clinical contact surfaces?
Touch surfaces
Transfer surfaces
Splash, splatter and droplet surfaces
What are Touch Surfaces and some examples?
Are directly touched
Dental light handles, computers, drawer handles, telephones, pens
What are Transfer Surfaces and some examples?
Touched by contaminated instruments
Trays, handpiece holders
What are Splash, Splatter and Droplet Surfaces and some examples?
Don’t contact staff or contaminated instruments
Counter tops
How often are Touched and Transfer surfaces cleaned?
Barrier protected OR cleaned and disinfected BETWEEN CLIENTS
How often are Splash, Splatter and Droplet surfaces cleaned?
At least once daily
What is the purpose of surface barriers?
Prevent contamination of surfaces
Come in plastic bag or sticky tape form
What should surface barriers be resistant to?
Fluids
When should barriers be removed?
Between clients, while wearing gloves
When should surface barriers be used?
When surfaces are hard to clean and disinfect.
What is the purpose of single-use disposable items?
Reduce contamination between clients