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Urine
Description: Liquid waste produced by the kidneys through filtration of blood. Contains water, urea, inorganic salts, and metabolic waste products.
Common diseases/conditions tested:
Urinary tract infections (UTIs)
Diabetes mellitus
Kidney diseases
Glomerulonephritis
Preeclampsia
Collection methods:
First-morning urine
Random urine specimen
Clean-catch specimen
24-hour urine collection
Stool (Feces)
Solid or semisolid waste remaining after food is digested and absorbed in the gastrointestinal tract.
Common diseases/conditions tested:
Gastrointestinal infections (bacterial, viral, parasitic)
Colorectal cancer (occult blood testing)
Inflammatory bowel disease (IBD)
Collection: Collect in an appropriate clean container and avoid contamination.
Sweat
Fluid secreted by sweat glands containing water, sodium, chloride, and other electrolytes.
Common condition tested:
Cystic fibrosis
Collection: Standardized sweat test performed by trained personnel.
Pleural Fluid
Fluid found between the membranes surrounding the lungs.
Common diseases/conditions tested:
Pleural effusion
Pneumonia
Tuberculosis (TB)
Lung cancer
Heart failure
Collection procedure: Thoracentesis.
Synovial Fluid
Description: Viscous fluid found in joint cavities that reduces friction during movement.
Common diseases/conditions tested:
Septic arthritis
Gout
Rheumatoid arthritis
Collection procedure: Arthrocentesis.
Saliva
Description: Secretion from salivary glands containing water, electrolytes, enzymes such as amylase, and immunoglobulins.
Common tests:
Cortisol testing
Selected viral infection tests
Drug screening or monitoring
Collection: Saliva is collected in a designated container according to test instructions.
Cerebrospinal Fluid (CSF)
Description: Clear, colorless fluid that surrounds and protects the brain and spinal cord.
Common diseases/conditions tested:
Meningitis
Encephalitis
Multiple sclerosis
Subarachnoid hemorrhage
Collection procedure: Lumbar puncture (spinal tap).
Peritoneal Fluid
Fluid within the abdominal cavity surrounding the abdominal organs.
Common diseases/conditions tested:
Inflammation
Cirrhosis and ascites
Intra-abdominal cancers
Bowel perforation
Collection procedure: Paracentesis.
Amniotic Fluid
Clear, yellowish fluid surrounding the fetus in the amniotic sac during pregnancy.
Common conditions tested:
Chromosomal abnormalities, including Down syndrome
Neural tube defects
Fetal lung maturity
Collection procedure: Amniocentesis.
Pericardial Fluid
Fluid contained within the sac surrounding the heart.
Common diseases/conditions tested:
Pericarditis
Cardiac tamponade
Pericardial effusion, which may be malignant or infectious
Collection procedure: Pericardiocentesis.
Sputum
Description: Thick mucus or phlegm coughed up from the lower respiratory tract, including the trachea and bronchi.
Common diseases/conditions tested:
Pneumonia
Tuberculosis (TB)
Chronic obstructive pulmonary disease (COPD)
Collection: Cough a deep respiratory specimen into an appropriate sterile container. Saliva alone is not an adequate substitute.
Biopsy
A tissue sample or collection of cells extracted from the body for microscopic examination.
Common diseases/conditions investigated:
Cancers and tumors
Selected infections
Hepatitis-related tissue disease
Autoimmune disorders
Collection: Tissue is obtained using an appropriate biopsy procedure.
Semen
Male reproductive fluid containing spermatozoa suspended in seminal plasma rich in nutrients and enzymes.
Common tests/conditions:
Infertility
Post-vasectomy verification
Male reproductive tract infections
Collection: Collected in a designated container following laboratory instructions.
Gastric Juice
Strongly acidic fluid produced by gastric glands containing hydrochloric acid (HCl), pepsinogen, and mucus.
Common diseases/conditions tested:
Gastritis
Peptic ulcer disease
Helicobacter pylori infection
Collection: Obtained through an appropriate gastric sampling procedure.
Cervical/Vaginal Swabs
Cellular and mucosal samples collected from the cervix or vaginal walls.
Common diseases/conditions tested:
Cervical cancer and precancerous lesions (Pap smear)
Human papillomavirus (HPV)
Bacterial vaginosis
Candidiasis (yeast infection)
Sexually transmitted infections, including chlamydia and gonorrhea
Collection: Use the appropriate swab and collection technique for the requested test.
Blood
Description: Circulating bodily fluid consisting of plasma and cellular elements, including red blood cells, white blood cells, and platelets.
Common diseases/conditions tested:
Anemia
Infections and sepsis
Leukemia
Cardiovascular disease
Metabolic and endocrine disorders
Kidney and liver dysfunction
Collection: Usually collected by venipuncture or another approved method.
SAMPLE
SAMPLE is used to collect the patient’s history and background information.
SAMPLE - S
S = Signs and Symptoms
What you observe and what the patient reports.
Includes vital signs.
SAMPLE - A
A = Allergies
Food allergies
Medication allergies
Environmental allergies
SAMPLE - M
M = Medications
Prescription medications
Supplements
OTC (over-the-counter) medications
SAMPLE - P
P = Past Pertinent Medical History
Previous surgeries
Medical conditions
Cancer history
Family history
SAMPLE - L
L = Last Oral Intake
What the patient last ate or drank.
When the patient last ate or drank.
SAMPLE - E
E = Events Leading Up to the Incident
What happened before the illness, injury, or complaint.
OPQRST — CHIEF COMPLAINT ASSESSMENT
O = Onset
When did it start?
What were you doing when it started?
P = Provocation/Palliation
What makes it worse?
What makes it better?
Q = Quality
What does it feel like?
Examples: sharp, dull, aching, throbbing, or pulling.
R = Region/Radiation
Where is the pain?
Does it spread or move anywhere else?
S = Severity
How bad is it on a scale of 1–10?
T = Time
How long has it lasted?
Is it constant or does it come and go?
LOC — LOSS OF CONSCIOUSNESS
A temporary loss of awareness or responsiveness.
During assessment, determine whether it occurred and gather information about the event.
CUPS — TRIAGE CATEGORIES
C = Critical
Immediately life-threatening condition.
U = Unstable
Serious condition that may deteriorate rapidly.
P = Potentially Unstable
Condition that may worsen and requires assessment and monitoring.
S = Stable
No current signs of immediate instability.
MOI — MECHANISM OF INJURY
The way an injury occurred.
Examples:
Fall
Motor vehicle collision
Sports injury
Other traumatic event
DCAP-BTLS — TRAUMA ASSESSMENT
D = Deformities
Abnormal shape or position of a body part.
C = Contusions
Bruises caused by injury.
A = Abrasions
Scrapes on the skin.
P = Punctures/Penetrations
Injuries where an object pierces the skin or enters tissue.
B = Burns
Tissue injuries caused by heat, chemicals, electricity, or other sources.
T = Tenderness
Pain or sensitivity when an area is examined.
L = Lacerations
Cuts or tears in tissue.
S = Swelling
An increase in tissue size, often caused by injury or inflammation.