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Antibiotic sensitivity test
a laboratory test which determines how effective antibiotic therapy is against bacterial infections
In vivo
inside
In vitro
outside
Diffusion method
Put a filter disc, or a porous cup/a bottomless cylinder containing measured quantity of drugs on the a slolid medium that has been seeded with test bacteria
Dilution method
Vary amount of antimicrobial substances incorporated into liquid or solid media
Kirby-Bauer test
Susceptibility test against more than one antibiotics by measuring size of inhibition zone
Bondi and Colleagues
Paper disks 1949
Kirby, Bauer, Sherris, and Tuck
filter paper disks 1966
Minimum inhibitory concentration
MIC meaning
sulfonamides and co-trimoxazole, proteus spp. beta-lactamase producing strep.
three exceptions of disc diffusion method
Minimum Bactericidal Concentration
MBC meaning
Minimum Lethal Concentration
MLC
Etest
comprises a predefined gradient of antibiotic concentrations on a plastic strip and can be used to determine the MIC of antibiotics, antifungal agents, and antimycobacterial agents