cell cycle stuff

wee1

inactivates m-cdk through phosphorylation

what does active M-Cdk do to wee1

inhibits it

cdc25

removes phosphates from m-cdk, activating it

m-cdk purpose

break down nuclear envelope

what gets ubiquitinated

p27, securin, M-cyclin

p27

holds S-Cdk inactive

M-cyclin levels rise

during interphase

at the end of M-phase, M-cyclin

tagged with ubiquitin and degraded

M-cyclin behavior in embryo vs non-embryo

M-cyclin rises immediately, vs flat in G1 and S, only rising in G2

at what stage are all cyclins low?

G1

Mitogens trigger synthesis of

G1/S-cyclins

Rb

binds to and inhibits TF

when do mitogens trigger G1/S synthesis?

in g1

S-Cdkk cyclin complexes initially inactive because

they are inactivated by a CKI, p27

SCF

ubiquitin ligase, ubiquitinates p27 (and mediates degradation of G1/S-cyclins)

APC at the end of mitosis

ubiuquitin ligase, ubiquitinates M-cyclin and S-cyclin

preRC contains

many compontent needed for DNA rep, but not all

once DNA replicated, which phase is complete?

S-phase (synthesis!)

what triggers the end of mitosis and driving cell into G1?

S-cyclin and M-cyclin both destroyed

APC targets

securin for degradation (anaphase promoting complex)