MLS ascp misc. tests

Homogenous

Mitochondrial

Peripheral

Centromere

Speckled and fine speckled

Nucleolar

What is the first marker positive in acute HIV

HIV RNA (plasma), 10-14 days post exporsure

What is the second marker in acute HIV after RNA

HIV-1 p24 antigen, 14-21 days after exposure

What are the last markers to rise in acute HIV after HIV RNA and p24

HIV antibodies- IgM then IgG 3-6 weeks after exposure

What is the first marker of hepatitis appearing 1-9 weeks after exposure

HBsAG- persistence beyond 6 months indicates chronic infection

What is the second marker of hepatitis appearing after HBsAg

HBV DNA (viral load)- direct, quantitative measure of active viral replication, higher number correlates with active proliferation and infectivity

What is the third marker of hepatitis which indicates high rates of viral replication during the active phase?

HBeAg

What is the fourth marker of hepatitis where high levels indicate acute primary infection or severe chronic hep

IgM anti-HBc

What is the fifth marker of hepatitis which appears early in acute infection and remains positive indefinitely as a footprint of past or current exposure

Total anti-HBc

What is the last hepatitis marker to appear which only does after HBsAg clears and signals recovery

Anti-HBs

Which mono marker is nonspecific that typically becomes detectable 5-10 days after symptoms and gradually disappear

Heterophile antibodies (monospot)

Which mono marker is highly active right at clinical onset and peaks rapidly and remains detectable for 4-6 weeks, making it the gold standard for current acute infection

Viral capsid antigen (VCA) IgM

Which marker for mono activates alongside VCA IgM during active stage of replication and drop to undetectable limits within 3-6 months

Early antigen (EA) IgG

Which mono marker begins its upward trajectory alongside VCA IgM during the acute phase, peaking between 2-4 weeks but does not disappear, it plateaus and remains active

VCA IgG

Which mono marker is entirely absent during the acute illness and slowly becomes active 2-4 months after symptom onset

EBNA IgG (epstein-barr nuclear antigen_

Which mono marker remains positive/active for life to provide immunity

VCA IgG and EBNA IgG

Which mono markers are permanently negative unless if a rare reactivation occurs

VCA IgM and heterophile antibodies