O curve, Bohr, LW-Burke, Fetal Hb/ allosteric effectors

0.0(0)
Studied by 0 people
call kaiCall Kai
learnLearn
examPractice Test
spaced repetitionSpaced Repetition
heart puzzleMatch
flashcardsFlashcards
GameKnowt Play
Card Sorting

1/23

flashcard set

Earn XP

Description and Tags

The rest of mb and hb ppt

Last updated 5:27 PM on 10/8/26
Name
Mastery
Learn
Test
Matching
Spaced
Call with Kai
Chat

No analytics yet

Send a link to your students to track their progress

24 Terms

1
New cards

What is oxygen dissociation curve?

shows how much oxygen a protein binds at different oxygen pressures (pO₂)


2
New cards

O curve in Mb

  • hyperbolic curve (smooth curve)

  • no cooperativity

  • Higher affinity for O2

  • Func: O storage in muscles


<ul><li><p><strong>hyperbolic</strong> curve (smooth curve)</p></li><li><p><strong>no cooperativity</strong></p></li></ul><ul><li><p><strong>Higher</strong> affinity for O2</p></li><li><p><mark data-color="yellow" style="background-color: yellow; color: inherit;">Func: O storage in muscles</mark></p></li></ul><p></p>
3
New cards

Oxygen Dissociation curve Hb

  • sigmoidal curve (s curve)

  • cooperative(4 subunits)

  • Lower affinity for O2

Func: O transport in blood

<ul><li><p><strong>sigmoidal</strong> curve (s curve)</p></li><li><p><strong>cooperative</strong>(4 subunits)</p></li><li><p><strong>Lower</strong> affinity for O2</p></li></ul><p><mark data-color="yellow" style="background-color: yellow; color: inherit;">Func: O transport in blood</mark></p>
4
New cards

R shift in O dissociation curve

Hb lets O₂ go (RELEASES O2)

  • lower affinity

  • more O2 release


5
New cards

L shift in O dissociation curve

Hb holding O2 tighter (LOVES O2)

  • higher affinity

  • less O2 release


6
New cards

cooperativity in Hb

Binding of one O₂ to Hb increases Hb's affinity for the next O₂


7
New cards

Bohr effect?

Increased H⁺ or CO₂ →promotes O₂ release from hb

8
New cards

source of H+ and CO2? (Bohr)

  • H+ = when CO2 reacts w/ water

  • CO2= produced during cellular respiration


9
New cards

P50?

the oxygen pressure where hemoglobin is 50% saturated

  • how well Hb holds o2


10
New cards
<p>Fetal Hb</p>

Fetal Hb

  • higher O affinity than adults

  • 2 alpha, 2 gamma

  • 2,3-DPG stabilizes


11
New cards
<p><span style="background-color: transparent;">Remove 2,3BPG and what happens to the P50 value?&nbsp;&nbsp;</span></p>

Remove 2,3BPG and what happens to the P50 value?  

  • p50 dec

  • left shift due to higher O2 affinity


12
New cards

what is Lineweaver-Burke plot?

  • MM curve

  • used to analyze enzyme kinetics and find Vmax & Km


13
New cards

LW- Burke: COMP

inter. on y axis

<p>inter. on y axis</p>
14
New cards

LW- Burke: UNCOMP

parallel

<p>parallel</p>
15
New cards

LW- Burke: NONCOMP

int. on x axis

<p>int. on x axis</p>
16
New cards

Allosteric effectors

  • Oxygen

  • pH/protons

  • CO2

  • 2,3-BPG

  • Loading and unloading O2 : Cooperativity delivers O2 to the tissues in response to small changes in the pO2


17
New cards

INC CO2

  • Shift: ➡ Right

  • P50: Increases

  • State: T state

  • Think: More CO₂ = tissues need more O₂ → Hb lets go of O₂.


18
New cards

DEC CO2

  • Shift: ⬅ Left

  • P50: Decreases

  • State: R state

  • Think: Less CO₂ = Hb holds O₂ tighter


19
New cards

INC 2,3- BPG

  • Shift: ➡ Right

  • P50: Increases

  • State: T state

  • Think: 2,3-BPG tells Hb: "Drop off your O₂."


20
New cards

DEC 2,3- BPG

  • Shift: ⬅ Left

  • P50: Decreases

  • State: R state

  • Think: Less 2,3-BPG = Hb holds onto O₂.


21
New cards

INC pH

  • Shift: ⬅ Left

  • P50: Decreases

  • State: R state

  • Think: Higher pH = less H⁺ = hold O₂.


22
New cards

DEC pH

  • Shift: ➡ Right

  • P50: Increases

  • State: T state

  • Think: Lower pH = more H⁺ = drop O₂.


23
New cards

R state

ready to grab O2

  • high affinity


24
New cards

T state

Time to let go O2

  • low affinity