N-linkedG-ationGluts&Hexokinases quiz

amino acid of helix7 in Glut2 that hypothetically toggles back and forth between a helix9 and a helix 12 phenylalanine pair

alanine

enzyme function in hepatocytes and Islet beta cells with high Km for retention of glucose

glucokinase

passively diffuses through the cell membrane but must be carried by myoglobin within the cytoplasm

molecular oxygen

monomer target to which the oligosaccharide is attached during N-linked glycosylation of proteins

Asparagine

amino acid of helix7 in high affinity (low Km) Gluts that positions glucose binding R-groups through its interaction with a conserved helix 9 histidine

serine

three of this are attached last to the oligosaccharide to be placed during N-linked glycosylation

glucose

insulin inducible glucose transporter found in muscle and adipose cells

Glut4

transports only dietary fructose from the intestinal lumen

Glut5

facilitates diffusion of BOTH glucose and fructose in liver and Islet beta cells

Glut2

required for glucose entry into erythrocytes and through blood-brain-barrier endothelial cells

Glut1

inhibits the first step of oligosaccharide synthesis preparatory to N-linked glycosylation

tunicamycin

performs secondary active transport of glucose out of the nephron filtrate at the proximal convoluted tubule

SGLT2

high affinity glucose transporter found in neuron membranes

Glut3

protein that directly performs N-linked glycosylation

oligosaccharide transferase

first two monosaccharides attached to dolichol pyrophosphate

N-acetyl-glucosamine

performs secondary active transport to concentrate glucose within brush border membrane enterocytes

SGLT1

binds to lumenal glycoproteins until glucoses are removed from the attached N-linked glycosylation

calreticulin

enzyme function in skeletal muscle with two active domains required to retain glucose upon entry through Glut4

hexokinase II

polyisoprene on which oligosaccharides are built prior to N-linked glycosylation alanine

dolichol

What SRP54/GTP first binds to during the production of Gluts at the rER

anchor peptide

rER lumen protein that first binds to incoming peptide to orchestrate folding events

BiP

most abundant monosaccharide found in N-linked glycosylation

mannose

performs the requisite ion gradient in the cell membrane by primary active transport

Na/K pump