Staphylococcus Quiz Lab 13

Is Staphylococcus Gram positive or negitive?

Gram postive

Staphylococcus looks like...

sphereical grape like clusters

Oxygen tolerance of Staphylococcus

facultative anaerobes

What are the 3 tests for Staphylococcus?

1. Nasal cavity screening for S. aureus

2. Mannitol fermentation

3. Coagulase test

Why will only a few microorgansims grow on MSA except staph?

MSA has high salt concentrations that inhibit other bacteria

What does the manitol agar turning yellow mean? What if no change (stays red)?

Turning means S. aureus and S. saprophyticus present becuase they ferment the manitol creating an acidic condition as shown by pH indicator.

If no color change then S. epidermis present becuase it does not ferment manitol.

Coagulase positive is when...

a clump forms in the rabbit plasma

Of S. Aureus, S. Epidermidis, and S. saprophyticus, which is coagulase positive?

S. Aureus is positive

S. Epidemidis & S. saprophyticus are negitive

What does a positive latex test look like?

Clumping

a yellow halo on MSA pluscoagulase positive indicated theisolate is...

S. aureus