LAB PRACTICAL 1

ni Flashcard 1: Q: Simple stain dye
A: Methylene blue

Flashcard 2: Q: Charge on bacterial cells
A: Negative

Flashcard 3: Q: Charge on dyes used
A: Positive

Flashcard 4: Q: Purpose of aseptic technique
A: Prevents contamination

Flashcard 5: Q: Target circle location on slide
A: Bottom of the slide

Flashcard 6: Q: Tool for transferring bacteria
A: Inoculating loop

Flashcard 7: Q: Flammable items in aseptic technique
A: Inoculating loop, open test tube

Flashcard 8: Q: Step after air drying specimen
A: Heat fix

Flashcard 9: Q: Purposes of heat fixing
A: Stick microbes to slide, kill bacteria

Lab 4: Gram Stain and Cell Model

Flashcard 10: Q: Target molecule in Gram stain
A: Peptidoglycan

Flashcard 11: Q: Peptidoglycan in Gram positive bacteria
A: Thick, stains purple

Flashcard 12: Q: Peptidoglycan in Gram negative bacteria
A: Thin, stains pink/red

Flashcard 13: Q: Primary dye in Gram stain
A: Crystal violet

Flashcard 14: Q: Decolorizer in Gram stain
A: 95% alcohol

Flashcard 15: Q: Counterstain in Gram stain
A: Safranin

Flashcard 16: Q: Gram positive species
A: Staphylococcus aureus

Flashcard 17: Q: Gram negative species
A: Escherichia coli

Flashcard 18: Q: Error for Gram positive appearing negative
A: Over-decolorization

Lab 5: Acid Fast Stain

Flashcard 19: Q: Target molecule in acid fast stain
A: Mycolic acid

Flashcard 20: Q: Use of steam in acid fast staining
A: Yes

Flashcard 21: Q: Acid fast positive color
A: Pink

Flashcard 22: Q: Acid fast negative color
A: Blue

Flashcard 23: Q: Primary dye in acid fast stain
A: Carbolfuchsin

Flashcard 24: Q: Decolorizer in acid fast stain
A: Acid alcohol

Flashcard 25: Q: Counterstain in acid fast stain
A: Methylene blue

Flashcard 26: Q: Acid fast positive species
A: Mycobacterium smegmatis

Flashcard 27: Q: Acid fast negative species
A: Streptococcus agalactiae

Flashcard 28: Q: Error for acid fast positive appearing negative
A: Over-washing with acid alcohol