Untitled Flashcards Set

1.      What is the selective ingredient in the EMB Test?

-          Dyes

2.      What type of bacteria does the EMB select for?

-          Gram negative bacteria (coliform)

3.      What type of metabolism occur in a EMB test?

-          Fermentation

4.      What sugars are fermented in a EMB test?

-          Lactose and sucrose

5.      What is the purpose of the dyes in the agar of an EMB test?

-          To prevent growth of gram positive bacteria

6.      What are fecal coliforms?

-          Bacteria that are found in human colon and stool samples

7.      What is the EMB test useful for?

-          Helps see if water has been contaminated by sewage

8.      What are the carbon sources in a EMB test?

-          Lactose or sucrose

9.      What bacteria can tolerate the dyes but not ferment lactose or sucrose in emb test?

-          Salmonella typhimurium

10.  What bacteria agar lavender in a EMB test?

-          Salmonella typhimurium

11.  What bacteria can tolerate dyes and ferment lactorse or sucrose in emb test?

-          Klebsiella aerogenes

12.  What bacteria agar is dark purple in emb test?

-          Klebsiella aerogenes

13.  What bacteria tolerates dyes and strongly ferments lactose or sucrose in emb test?

-          E coli

14.  What bacteria agar is metallic green in emb test?

-          E coli

1.      What does the HE test help idenfity?

-          Bacteria that cause food poisoing

2.      What is the selective ingredient in HE test?

-          Bile

3.      What type of metabolism happens in HE test?

-          Fermentation

4.      What sugars does the agar contain in the HE test?

-          Lactose. Sucrose. And salicin

5.      What bacteria can tolerate bile and ferment sugars in HE test?

-          E coli

6.      What bacteria turns orange on agar in HE test?

-          E coli

7.      What bacteria can tolerate bile and not ferment sugar or reduce sulfur in HE test?

-          Shigella felxneri

8.      What bacteria turns blue/green in HE test?

-          Shigella flexneri

9.      What bacteria can tolerate bile, reduce sulfur, and does not ferment sugars in HE test?

-          Salmonella typhimurium

10.  What bacteria turns blue/green + black in HE test?

-          Salmonella typhimurium

11.  What chemical reaction must take place for hydrogen sulfide to react with ferric ammonium citrate in HE test?

-          Reduction of sulfur

1.      What sugar is used in the OF test in lab?

-          Glucose

2.      What is the result of anaerobic metabolism in OF test?

-          Green on top chamber and yellow on bottom chamber

3.      What is the result of aerobic metablosim in OF test?

-          Yellow on top chamber and green on bottom chamber?

4.      What is the result of facultative metabolism in OF test?

-          Yellow on both chambers

5.      What metabolism occurs in E Coli in OF test?

-          Facultative metabolism

6.      What metabolism occurs in Pseudomonas aeruginosa in OF test?

-          Aerobic metabolism

1.      What sugar is used in lab for PR test?

-          Glucose

2.      What is the purpose of Durham tube in PR test?

-          It shows and forms bubble if bacteria can produce a gas

3.      What is the pH indicatior in PR test?

-          Phenol red

4.      What color will the broth be if the bacteria can ferment sugar producing acid in PR test?

-          Yellow/ orange and A+

5.      What color will the broth be if the bacteria can not ferment sugar or produce acid in PR test?

-          Red and A-

6.      What will happen in the tube if the bacteria can produce gas in PR test?

-          Bubble and G+

7.      What will happen in the tube if the bacteria cannot produce gas in PR test?

-          No bubble and G-

8.      What bacteria can ferment sugar producing acid and produce gas in PR test?

-          E coli A+/G+

9.      What bacteria can not ferment sugar or produce acid or gas in PR test?

-          Enterococcus faecalis A-/G-

1.      What pH does the citrate test for?

-          Basic pH

2.      A positive citrate test is able to?

-          Import and utilize citrate from environment turning blue

3.      A negative citrate test is able to?

-          Not import and utilize citrate from environment staying green

4.      What bacterias have a positive citrate test?

-          Klebsiella aerogenes, Pseudomonas aueroginosa, salmonella

5.      What bacteria have a negative citrate test?

-          E coli

1.      What makes the bacteria red in motility test?

-          TTC

2.      What structure is being tested for in motility test?

-          Flagella

3.      What bacteria is not motile and does not have flagella?

-          Klebsiella pneumoniae

4.      What bacteria is motile and has flagella?

-          Proteus mirabilis

1.      What bacteria contains urease and hydrolyzes urea turing bright pink in Urease test?

-          Proteus mirabliis and Klebsiella penumoniae

2.      What bacteria does not contain urease in Urease test staying yellow/orange ?

-          E coli, enteroccous faecalis, and pseudomonas aeruginosa

3.      What enzyme is tested in the Urease test?

-          Urease

4.      What is important about the Urease test?

-          It does not tell you whether someone has a UTI it tells you what might be causing the UTI

1.      What does the S stand for in SIM Test?

-          Sulfur reduction

2.      What does the I stand for in SIM test?

-          Indole production

3.      What does M stand for in SIM test?

-          Motility

4.      What color will the agar be if reduction sulfur occurs in SIM test?

-          Dark

5.      What color will the agar be if no reduction sulfur occurs in SIM test?

-          Pale yellow

6.      What color will the regent layer be in the bacteria contains tryptophanase and produce indole?

-          Red

7.      What color will the regent layer be in the bacteria does not contains tryptophanase?

-          Colorless

8.      What bacteria can reduce sulfur and does not contain tryptophanase?

-          Proteus mirabilis S+/I-

9.      What bacteria cannot reduce sulfur and does not contain tryptophanase?

-          Klebsiella aerogenes S-/I-

10.  What bacteria cannot reduce sulfur but contain tryptophanase producing indole?

-          E Coli S-/I+