All chemicals are potentially hazardous: eye goggles must be worn
Take the food sample and grind with distilled water using a mortar and pestle. We want to make a paste.
Transfer the paste to a beaker and add more distilled water. Stir so the chemicals in the food dissolve in the water.
Filter the solution to remove suspended food particles.
Starch
Add to test tube
Add a few drops of iodine solution
If starch is present, the orange iodine solution will turn blue/black
Sugars
Add 10 drops of Benedict’s solution (which is a blue colour)
Place test tube containing solution into a beaker and half-fill the beaker with hot water from a kettle
Leave for around 5 minutes
Green/yellow - small amount of sugar, red - lots of sugar present
Only works for certain sugars (reducing sugars)
Protein
Add 2cm3 to test tube
Add 2cm3 of biuret solution (blue colour)
If protein is present, will turn to a purple or lilac colour
Lipids
Don’t filter solution
2cm3 added to test tube
Add a few drops of water and a few drops of ethanol
Gently shake
White cloudy emulsions form if lipids are present
Food Tests