Last saved 23 days ago
KW

Food Tests

All chemicals are potentially hazardous: eye goggles must be worn


  1. Take the food sample and grind with distilled water using a mortar and pestle. We want to make a paste.

  2. Transfer the paste to a beaker and add more distilled water. Stir so the chemicals in the food dissolve in the water.

  3. Filter the solution to remove suspended food particles.


Starch

  • Add to test tube

  • Add a few drops of iodine solution

  • If starch is present, the orange iodine solution will turn blue/black


Sugars

  • Add to test tube

  • Add 10 drops of Benedict’s solution (which is a blue colour)

  • Place test tube containing solution into a beaker and half-fill the beaker with hot water from a kettle

  • Leave for around 5 minutes

  • Green/yellow - small amount of sugar, red - lots of sugar present

  • Only works for certain sugars (reducing sugars)


Protein

  • Add 2cm3 to test tube

  • Add 2cm3 of biuret solution (blue colour)

  • If protein is present, will turn to a purple or lilac colour


Lipids

  • Don’t filter solution

  • 2cm3 added to test tube

  • Add a few drops of water and a few drops of ethanol

  • Gently shake

  • White cloudy emulsions form if lipids are present




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Food Tests

All chemicals are potentially hazardous: eye goggles must be worn

  1. Take the food sample and grind with distilled water using a mortar and pestle. We want to make a paste.

  2. Transfer the paste to a beaker and add more distilled water. Stir so the chemicals in the food dissolve in the water.

  3. Filter the solution to remove suspended food particles.

Starch

  • Add to test tube

  • Add a few drops of iodine solution

  • If starch is present, the orange iodine solution will turn blue/black

Sugars

  • Add to test tube

  • Add 10 drops of Benedict’s solution (which is a blue colour)

  • Place test tube containing solution into a beaker and half-fill the beaker with hot water from a kettle

  • Leave for around 5 minutes

  • Green/yellow - small amount of sugar, red - lots of sugar present

  • Only works for certain sugars (reducing sugars)

Protein

  • Add 2cm3 to test tube

  • Add 2cm3 of biuret solution (blue colour)

  • If protein is present, will turn to a purple or lilac colour

Lipids

  • Don’t filter solution

  • 2cm3 added to test tube

  • Add a few drops of water and a few drops of ethanol

  • Gently shake

  • White cloudy emulsions form if lipids are present