VT

The Chemistry of Life

The Chemistry of Life

Proteins

  • Biological macromolecules composed of amino acids.

Lipids

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  • Fatty acids, triglycerides, phospholipids, steroids.

Carbohydrates

  • Includes sugars and polymers of sugars (e.g. starch, cellulose).

Nucleic Acids

  • Polymers made of nucleotides, such as DNA and RNA.


Lipids

1. Fats

  • Functions:

    • Storage of energy

    • Insulation and protection

2. Membrane Lipids

  • Major component of cell membranes.

3. Steroids

  • Characterized by a carbon skeleton with four fused rings.


Fats

  • Composed of two molecule types:

    1. Fatty Acids

    • Structure: Long hydrocarbon chains with a carboxyl group (e.g., Palmitic acid, oleic acid).

    1. Glycerol

    • Structure: A three-carbon alcohol.


Fatty Acids

  • Types:

    • Saturated

    • Unsaturated

      • Unsaturated Fatty Acids:

      • Have kinks due to double bonds, preventing them from stacking.

      • Tend to remain liquid at room temperature.

      • Saturated Fatty Acids:

      • No double bonds; solid at room temperature.

Examples:
  • Saturated: Palmitic acid

  • Monounsaturated: Oleic acid

  • Polyunsaturated: Linoleic acid


“Trans” Fat and Health

  • Cis and Trans Configurations:

    • Cis configuration introduces a kink, allowing for fluidity.

    • Trans configuration prevents kinks, making the fatty acid straighter.

  • In biological systems, most fatty acids are in the cis form.

  • Esterification:

    • Fatty acids attach covalently to other molecules via this dehydration reaction.

  • Health Implications:

    • Trans fats increase LDL (“bad” cholesterol) and decrease HDL (“good” cholesterol).


Triglycerides (fats)

  • Formed from three fatty acids covalently bonded to glycerol through dehydration synthesis, resulting in the release of water molecules (
    3H_2O).

  • Structure:

    • Glycerol + 3 Fatty Acids → Triglyceride.


Hydrolysis

  • Process Description:

    • The addition of water to break down molecules (opposite of dehydration synthesis).

    • Example Reaction:
      ext{Fatty acid} + ext{Glycerol} + H_2O o ext{Hydrolyzed Product}


Phospholipids

  • Components:

    1. Fatty Acids.

    2. Glycerol.

    3. Polar Head Group:

    • Example: Phosphocholine.

  • Phospholipids undergo dehydration synthesis similar to triglycerides, forming a bilayer structure in cell membranes.

Amphiphilic Nature

  • Description:

    • Phospholipids possess both hydrophilic (polar head) and hydrophobic (nonpolar tails) regions, critical for the formation of the phospholipid bilayer.


Cholesterol

  • Role and Function:

    • Most common steroid in humans.

    • Provides structure to cell membranes.

    • Precursor to steroid hormones (like testosterone and estradiol).

  • Characteristic: Mostly insoluble in water.

  • Emulsification Role:

    • Cholesterol acts as an emulsifier in bile, aiding in fat digestion alongside other components like taurocholic acid and phospholipids.


Carbohydrates

  • General formula: C:H:O in the ratio of 1:2:1.

  • Monosaccharides: Simple sugars with two variations of the carbonyl group: Aldoses (e.g., glucose) and Ketoses (e.g., fructose).

Cyclic Monosaccharides

  • Rarely Exist in Linear Form:

    • Fisher Projection: Linear form representation.

    • Haworth Projection: Cyclic representation where hydroxyl groups are either above or below the plane of the ring.

    • Example of Equilibrium: In aqueous solution, alpha and beta glucose exist in a 36:64 ratio.


Various Common Monosaccharides

  • Examples of Common Hexoses:

    • Glucose, Fructose, Galactose, Mannose (epimers).


Disaccharides

  • Formed via dehydration synthesis of two monosaccharides connected by glycosidic bonds.

  • Example: Two alpha-glucose molecules form maltose via an alpha 1-4 glycosidic bond.


Polysaccharides

  • Starch: A polysaccharide formed by repeating eta -1,4 linkages of glucose produced exclusively by plants for energy storage; branched form called amylopectin also contains eta -1,6 bonds.

  • Glycogen: Similar to amylopectin, but with more frequent eta -1,6 branches; stored in liver and muscle cells.

  • Cellulose: Polymeric molecule with eta -1,4 bonds. Provides rigidity to plant cell walls; resistant to digestion as only certain microorganisms can break these bonds.

  • Chitin: Modified glucose (N-acetyl-glucosamine) structure; common in exoskeletons of arthropods.

  • Peptidoglycan: Component of bacterial cell walls; made of alternating N-acetyl-muramic acid subunits, crosslinked for strength.


Nucleic Acids

  • Composed of polymers of nucleotides which consist of:

    1. Nitrogenous Base.

    2. 5-Carbon Sugar (ribose or deoxyribose).

    3. Phosphate Group (mono, di, or tri).


Nucleotide Hydrolysis

  • Hydrolysis of ATP (adenosine triphosphate) involves breaking phosphoanhydride bonds, releasing energy crucial for cellular processes.


Nitrogenous Bases

  • Five types included:

    • Adenine (A) and Guanine (G): Purines.

    • Thymine (T), Cytosine (C), and Uracil (U): Pyrimidines.

Differences Between RNA and DNA

  • RNA contains Uracil (U), while DNA contains Thymine (T).

  • RNA includes ribose; DNA has deoxyribose.

  • DNA is typically double-stranded; RNA is usually single-stranded.


Directionality of Polynucleotides

  • Nucleic acids exhibit directionality with 5’ phosphate end and the 3’ hydroxyl end, causing them to hybridize in an antiparallel fashion.


DNA Structural Features

  • Double Helix: Formed by complementary base pairing, involving hydrogen bonds.

  • Base Pairing Rules:

    • Adenine pairs with Thymine (for DNA).

    • Cytosine pairs with Guanine.


Cell Theory

  • Basic Principles:

    1. All living things are composed of cells.

    2. Cells arise from pre-existing cells.

    3. Cells are the fundamental units of life, demonstrating all vital functions.


Eukaryotic Cells vs. Prokaryotic Cells

  • Eukaryotic Cells: Contain a nucleus and membrane-bound organelles.

  • Prokaryotic Cells: Lack a nucleus; contain a nucleoid with genetic material but no membrane-bound structures.


The Plasma Membrane

  • Comprised primarily of phospholipids arranged in a bilayer, creating a barrier to polar substances.

  • The arrangement of hydrophobic tails and hydrophilic heads provides selective permeability essential for cellular function.