-Where can microbiology specimens be collected?
-What are the common specimens collected for culture include?
-Some of the areas of the body or fluids should be sterile and lack microbiota, which are those sterile areas?
-Go over the different specimens we can collect and why we collect those specimens (reasons). Example Throat specimen for Strep throat, looking for Streptococcus pyogenes.
-When we are collecting specimens, where should the label go and what do we must record?
-What is the importance of writing the site of the specimen on the container?
-If specimens cannot be cultured immediately, is refrigeration not good enough/effective for anaerobic organisms, CSF (cerebrospinal fluid) or genital cultures? (Yes or no). If yes, then where should we store temperature?
-What are the characteristics of group A streptococcus?
-What is the common microorganism that is responsible for UTI (Urinary Tract Infection)? Is is a gram positive or gram negative and what shape?
-What type of urine specimen do we have to collect for urine culture and sensitivity (C&S). What is the procedure for collecting it?
-On what culture media should we inoculate urine specimens?
-What is a colony count, how do we calculate it and is it clinically significant?
-For respiratory specimens such as sputum, what are the requirements and how to collect it?
-How do we know if the sputum specimen is acceptable? (Look at slide 43/44 of Lesson Content 6 -go over the squamous epithelial cells part).
-What is clostridium difficile
-What can you detect from fecal specimens?
-What is the best method for finding pinworm organisms in children?
-What does O&P stand for?
-What is the fixative found in the jar for O&P stool?
-A stool for O&P can be collected in which containers containing what?
-What is the flotation technique and what are the two solutions used?
-What is the sedimentation method?
-Go over blood cultures
-What is the most critical part of blood culture collection?