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AP BIO Unit 3 Review - Cellular Energetics

Enzymes and Reactions

Entropy -A measure of disorder, or randomness

  • decreased by nonspontaneous reactions

  • increase by heat/energy released

Energy Coupling - In cellular metabolism, the use of energy released from an exergonic reaction to drive an endergonic reaction

  • Photosynthesis transforms light energy into chemical energy stored in glucose, and cellular respiration releases the energy from glucose to build ATP, which does the work of life

Active Site - The specific region of an enzyme that binds the substrate and that forms the pocket in which catalysis occurs

Because systems at equilibrium are at a minimum of G and can do no work, a cell that has reached metabolic equilibrium is dead! The fact that metabolism as a whole is never at equilibrium is one of the defining features of life

Types of reactions

Catabolic/Exergonic/Spontaneous

doesn’t require the input of energy

Anabolic/Endergonic/Nonspontaneous

requires the input of energy

Enzymes lower the activation energy required for a reaction

  • specific for the reactions they catalyze

  • orients substrates properly

  • stretch and stress bonds to be broken

  • provides an ideal environment (pH, temperature)

Properties of Enzymes

Environment conditions

Shape changes

Inhibition


Oscillation


Feedback

End product allosterically inhibit - prevents the cell from creating more product than necessary

  • ATP binds to several catabolic enzymes allosterically, lowering their affinity for substrate and thus inhibiting their activity. ADP, however, functions as an activator of the same enzymes. This is logical because catabolism functions in regenerating ATP. If ATP production lags behind its use, ADP accumulates and activates the enzymes that speed up catabolism, producing more ATP. If the supply of ATP exceeds demand, then catabolism slows down as ATP molecules accumulate and bind to the same enzymes, inhibiting them


Cellular Respiration

C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy

Glycolysis

  • A series of reactions that ultimately splits glucose into pyruvate. Glycolysis occurs in almost all living cells, serving as the starting point for fermentation or cellular respiration

  • occurs in the cytoplasm

Pyruvate Oxidation

  • A biochemical reaction that involves the oxidation of pyruvate to create acetyl CoA

  • occurs in the mitochondria

Citric Acid Cycle

  • A chemical cycle involving eight steps that completes the metabolic breakdown of glucose molecules begun in glycolysis by oxidizing acetyl CoA (derived from pyruvate) to carbon dioxide; occurs within the mitochondrion in eukaryotic cells and in the cytosol of prokaryotes; together with pyruvate oxidation, the second major stage in cellular respiration

  • occurs in the mitochondria

Oxidative Phosphorylation

  • The production of ATP using energy derived from the redox reactions of an electron transport chain; the third major stage of cellular respiration

  • occurs in the mitochondria

  • ETC in the inner membrane (NADH and FADH2 drops of elections and H+ goes to the intermembrane space through proteins)

Fermentation

  • A catabolic process that makes a limited amount of ATP from glucose (or other organic molecules) without an electron transport chain and that produces a characteristic end product, such as ethyl alcohol or lactic acid

Importantce of Oxygen

  • O2 is the final electron acceptor

Anaerobic -A catabolic pathway in which inorganic molecules other than oxygen accept electrons at the “downhill” end of electron transport chains

Aerobic - A catabolic pathway for organic molecules, using oxygen (O2) as the final electron acceptor in an electron transport chain and ultimately producing ATP. This is the most efficient catabolic pathway and is carried out in most eukaryotic cells and many prokaryotic organisms

Alcohol Fermentation - Glycolysis followed by the reduction of pyruvate to ethyl alcohol, regenerating NAD+ and releasing carbon dioxide

Lactic Acid Fermentation - Glycolysis followed by the reduction of pyruvate to lactate, regenerating NAD+ with no release of carbon dioxide

Photosynthesis

6CO2 + 12H2O + Light Energy → C6H12O6 + 6O2

Light Reactions

The first of two major stages in photosynthesis (preceding the Calvin cycle). These reactions, which occur on the thylakoid membranes of the chloroplast or on membranes of certain prokaryotes, convert solar energy to the chemical energy of ATP and NADPH, releasing oxygen in the process

  • energy harvesting phase

Photophosphorylation

The process of generating ATP from ADP and phosphate by means of chemiosmosis, using a proton-motive force generated across the thylakoid membrane of the chloroplast or the membrane of certain prokaryotes during the light reactions of photosynthesis

Calvin Cycle

The second of two major stages in photosynthesis (following the light reactions), involving fixation of atmospheric CO2 and reduction of the fixed carbon into carbohydrate

  • forms sugar

Carbon Fixation

The initial incorporation of carbon from CO2 into an organic compound by an autotrophic organism (a plant, another photosynthetic organism, or a chemoautotrophic prokaryote)

D

AP BIO Unit 3 Review - Cellular Energetics

Enzymes and Reactions

Entropy -A measure of disorder, or randomness

  • decreased by nonspontaneous reactions

  • increase by heat/energy released

Energy Coupling - In cellular metabolism, the use of energy released from an exergonic reaction to drive an endergonic reaction

  • Photosynthesis transforms light energy into chemical energy stored in glucose, and cellular respiration releases the energy from glucose to build ATP, which does the work of life

Active Site - The specific region of an enzyme that binds the substrate and that forms the pocket in which catalysis occurs

Because systems at equilibrium are at a minimum of G and can do no work, a cell that has reached metabolic equilibrium is dead! The fact that metabolism as a whole is never at equilibrium is one of the defining features of life

Types of reactions

Catabolic/Exergonic/Spontaneous

doesn’t require the input of energy

Anabolic/Endergonic/Nonspontaneous

requires the input of energy

Enzymes lower the activation energy required for a reaction

  • specific for the reactions they catalyze

  • orients substrates properly

  • stretch and stress bonds to be broken

  • provides an ideal environment (pH, temperature)

Properties of Enzymes

Environment conditions

Shape changes

Inhibition


Oscillation


Feedback

End product allosterically inhibit - prevents the cell from creating more product than necessary

  • ATP binds to several catabolic enzymes allosterically, lowering their affinity for substrate and thus inhibiting their activity. ADP, however, functions as an activator of the same enzymes. This is logical because catabolism functions in regenerating ATP. If ATP production lags behind its use, ADP accumulates and activates the enzymes that speed up catabolism, producing more ATP. If the supply of ATP exceeds demand, then catabolism slows down as ATP molecules accumulate and bind to the same enzymes, inhibiting them


Cellular Respiration

C6H12O6 + 6O2 → 6CO2 + 6H2O + Energy

Glycolysis

  • A series of reactions that ultimately splits glucose into pyruvate. Glycolysis occurs in almost all living cells, serving as the starting point for fermentation or cellular respiration

  • occurs in the cytoplasm

Pyruvate Oxidation

  • A biochemical reaction that involves the oxidation of pyruvate to create acetyl CoA

  • occurs in the mitochondria

Citric Acid Cycle

  • A chemical cycle involving eight steps that completes the metabolic breakdown of glucose molecules begun in glycolysis by oxidizing acetyl CoA (derived from pyruvate) to carbon dioxide; occurs within the mitochondrion in eukaryotic cells and in the cytosol of prokaryotes; together with pyruvate oxidation, the second major stage in cellular respiration

  • occurs in the mitochondria

Oxidative Phosphorylation

  • The production of ATP using energy derived from the redox reactions of an electron transport chain; the third major stage of cellular respiration

  • occurs in the mitochondria

  • ETC in the inner membrane (NADH and FADH2 drops of elections and H+ goes to the intermembrane space through proteins)

Fermentation

  • A catabolic process that makes a limited amount of ATP from glucose (or other organic molecules) without an electron transport chain and that produces a characteristic end product, such as ethyl alcohol or lactic acid

Importantce of Oxygen

  • O2 is the final electron acceptor

Anaerobic -A catabolic pathway in which inorganic molecules other than oxygen accept electrons at the “downhill” end of electron transport chains

Aerobic - A catabolic pathway for organic molecules, using oxygen (O2) as the final electron acceptor in an electron transport chain and ultimately producing ATP. This is the most efficient catabolic pathway and is carried out in most eukaryotic cells and many prokaryotic organisms

Alcohol Fermentation - Glycolysis followed by the reduction of pyruvate to ethyl alcohol, regenerating NAD+ and releasing carbon dioxide

Lactic Acid Fermentation - Glycolysis followed by the reduction of pyruvate to lactate, regenerating NAD+ with no release of carbon dioxide

Photosynthesis

6CO2 + 12H2O + Light Energy → C6H12O6 + 6O2

Light Reactions

The first of two major stages in photosynthesis (preceding the Calvin cycle). These reactions, which occur on the thylakoid membranes of the chloroplast or on membranes of certain prokaryotes, convert solar energy to the chemical energy of ATP and NADPH, releasing oxygen in the process

  • energy harvesting phase

Photophosphorylation

The process of generating ATP from ADP and phosphate by means of chemiosmosis, using a proton-motive force generated across the thylakoid membrane of the chloroplast or the membrane of certain prokaryotes during the light reactions of photosynthesis

Calvin Cycle

The second of two major stages in photosynthesis (following the light reactions), involving fixation of atmospheric CO2 and reduction of the fixed carbon into carbohydrate

  • forms sugar

Carbon Fixation

The initial incorporation of carbon from CO2 into an organic compound by an autotrophic organism (a plant, another photosynthetic organism, or a chemoautotrophic prokaryote)

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