Cell Theory: All organisms are composed of one or more cells; the cell is the basic unit of structure and organization in organisms; all cells come from pre-existing cells.
Anton van Leeuwenhoek discovered bacteria.
Robert Hooke named the cell.
Theodor Schwann realized all animals are made of cells.
Scientific Investigation: Sensitivity of the Skin
Scientific Question: Which part of the arm is the most sensitive?
Independent Variable: Part of the arm.
Dependent Variable: Number of points felt correctly.
Control Variable: Same type of wire, same person.
Using a Microscope
To see cells in detail, a microscope is needed to magnify the object.
Key parts of a microscope:
Eyepiece lens: To observe your specimen(cell).
Coarse adjustment knob: To move the stage up and down.
Fine adjustment knob: To sharpen/unblur the image.
Base: To support the whole microscope.
Objective lens: To magnify the image.
Stage: Place the glass slide on it.
Diaphragm: To control the brightness of the light.
Light Source: Light shines onto specimen.
Viewing Cells Under a Microscope
Onion Cell Preparation: Use a knife to cut the onion -> Proceed with using a scalpel to obtain a thin layer -> Place the thin layer (specimen) on the glass slide -> Add 2 - 3 drops of iodine and cover the specimen with a cover slip -> Place the glass slide onto the lowered stage and start with the lowest magnification.
Cheek Cell Preparation: Gently scrape the inside of your mouth with a toothpick -> Smear the toothpick on the center of the glass slide -> Add 1 - 2 drops of methylene blue and cover the specimen with a cover slip -> Remove any excess liquid with a paper towel -> Place the glass slide onto the lowered stage and start with the lowest magnification.
Safety rules include gently scraping cheeks, throwing toothpicks immediately, and using low concentration of methylene blue.