Glucose to Pyruvate Sp. 25
Glucose to Pyruvate Overview
Chapters: 7.2-7.4 of Prescott's Principles of Microbiology
Authors: Joanne Willey, Kathleen Sandman
Learning Objectives
Familiarize with energy carriers and their role in electron transport during respiration.
Focus on: ATP and substrate-level phosphorylation.
Differentiate between heterotrophs and chemotrophs based on energy and electron sources.
Understand aerobic respiration pathways for glucose breakdown:
Glycolysis: Inputs, outputs, key enzymes discussed in class.
Entner Doudoroff pathway: General understanding and outputs.
Pentose Phosphate Pathway: General understanding and outputs.
Review of Metabolism
Catabolic pathways break down large molecules to store energy in small carriers like ATP and NADH.
ATP and nucleotide triphosphates: Store energy in phosphodiester bonds.
NADH, NADPH, FADH2: Each stores energy as electron pairs, contributing to reducing power.
Enzymes lower the ΔG to facilitate reactions by coupling energy transfer to biosynthesis and cell function.
Microbial Metabolism
Energy is essential for movement and growth in all living cells.
Catabolism: Breakdown of complex molecules into simpler ones; provides energy for:
Anabolism: Constructing new products.
Energy from catabolism is primarily in the form of ATP.
Electron Acceptors
Many microbes can grow in the presence of molecular oxygen (O2), using it as a terminal electron acceptor in aerobic respiration.
Energy Carriers and Electron Transfer
Energy transfer reactions in cells involve carriers:
Molecules that capture and release small amounts of energy reversibly.
Notable examples: NADH, FADH2, and ATP.
NADH: Acts as an electron donor; NAD+: Acts as an electron acceptor.
Formation of NADH
NADH can carry 2-3 times as much energy as ATP.
NADH: Reduced form.
NAD+: Oxidized form.
Reaction: NAD+ + 2H+ + 2e– → NADH + H+
The reduction involves the gain of two electrons and two hydrogen atoms.
Flavin Adenine Dinucleotide (FAD)
FAD: Another coenzyme involved in electron transfer.
FADH2: Reduced form, distilled from FAD via two electrons and protons.
Adenosine Triphosphate (ATP)
Structure: Composed of a base, a sugar, and three phosphates.
Formation: ADP plus inorganic phosphate (Pi) creates ATP, representing the cell's energy currency.
Substrate-Level Phosphorylation: Direct transfer of phosphate to another molecule.
ATP Energy Transfer
ATP hydrolysis releases energy in three ways:
Hydrolysis releasing inorganic phosphate (Pi).
Hydrolysis releasing pyrophosphate (PPi).
Phosphorylation of an organic molecule.
ATP is fully renewable energy source as it facilitates cellular functions from muscle contractions to biosynthesis.
Catabolism in Microbes
Microbes can exploit numerous substrates:
Polysaccharides broken down to pyruvate.
Pyruvate can undergo fermentation or further catabolism to CO2 and H2O in the TCA cycle.
Lipids and amino acids can catabolize to glycerol and acetate.
Nutritional Types of Organisms
Heterotrophs: Use organic molecules as carbon and energy sources.
Autotrophs: Use CO2 as their primary carbon source.
Phototrophs: Derive energy from light.
Chemotrophs: Obtain energy from chemical compounds.'
Lithotrophs: Use reduced inorganic substances as electron sources; Organotrophs: Use electrons from organic compounds.
Chemoorganotrophic Pathways
Chemoorganotrophs also termed chemoheterotrophs, employ pathways including:
Aerobic respiration
Anaerobic respiration
Fermentation
Respiration Mechanisms
Main respiration types:
Aerobic respiration: With oxygen as the final electron acceptor.
Anaerobic respiration: Different oxidized molecules serve as terminal electron acceptors.
Electron transport chain plays a pivotal role in respiration for ATP synthesis via proton motive force (PMF).
Fermentation Process
Utilizes endogenous electron acceptors, typically intermediates from the catabolism of the organic energy source (e.g., pyruvate).
No electron transport chain involved; ATP is synthesized solely through substrate-level phosphorylation.
Major Catabolism Pathways for Glucose
Prokaryotes metabolize glucose utilizing three primary pathways:
Glycolysis: Produces 2 ATP and 2 NADH.
Entner-Doudoroff pathway: Yields 1 ATP, 1 NADH, and 1 NADPH.
Pentose phosphate pathway: Generates 1 ATP and 2 NADPH.
Fermentation completes glucose catabolism without an electron transport chain.
Pathway Summaries
Embden-Meyerhof Pathway: Most common for glucose degradation to pyruvate in aerobic respiration.
Energy Investment Phase: Requires 2 ATP to activate glucose.
Energy Yield Phase: Produces NADH and ATP, net gain of 2 ATP.
Individual Pathway Details
Entner-Doudoroff Pathway:
Key for some Gram-negative bacteria; replaces 6-carbon phase in glycolysis.
Yields: 1 ATP, 1 NADPH, 1 NADH per glucose molecule.
Pentose Phosphate Pathway:
Functioning alongside glycolysis or Entner-Doudoroff.
Produces NADPH and sugars essential for biosynthesis.
Summary of Glycolysis
Converts one glucose molecule to two pyruvate molecules.
Net gain: 2 ATP, 2 NADH; 2 ATP expended, 4 ATP harvested.
Final Notes on Metabolism
All organisms seek ATP as an energy currency, reducing power, and precursor metabolites.
Essential pathways contribute to metabolic efficiency by integrating varied nutrient utilizations.