Genetics- Chapter 2: Mitosis & Meiosis

Chapter Overview

• Mitosis and Meiosis: Fundamental processes in genetic material transmission.

• Chromosomes: Composed of nucleic acid DNA, organized structures that carry genetic information.

Learning Objectives

• 2.2 Chromosomes exist in homologous pairs in diploid organisms.

• 2.3 Mitosis partitions chromosomes into dividing cells.

• 2.4 Meiosis creates haploid gametes and spores, enhancing genetic variation.

• 2.5 Variation in gamete development: spermatogenesis vs. oogenesis.

• 2.6 Importance of meiosis in sexual reproduction of diploid organisms.

• 2.7 Insights from electron microscopy on mitotic and meiotic chromosomes.

Introduction to Genetic Material

• Genetic Material in Living Organisms: DNA organized into chromosomes.

• Eukaryotes: Genetic transmission primarily involves mitosis and meiosis, excluding viruses.

Mitosis vs. Meiosis

• Mitosis: Produces two identical cells (same chromosome number as parent cell).

• Meiosis: Produces sex cells (gametes or spores), reducing chromosome number by half (haploid).

• Chromatin: Chromosomes uncoil during nondivisional periods, forming a diffuse network within the nucleus.

2.2 Homologous Chromosomes

• Definition: Chromosomes in diploid organisms exist in homologous pairs; similar but not identical.

• Alleles: Different versions of the same gene located at corresponding loci on homologous chromosomes.

Centromeres

• Function: Constricted regions on chromosomes critical for chromosome appearance and behavior during division.

• Types:

  • Metacentric: Centromere in middle.

  • Submetacentric: Centromere slightly off-center.

  • Acrocentric: Centromere near one end.

  • Telocentric: Centromere at the end.

Karyotype

• Visual representation of physical chromosome pairs in a cell; humans have 46 chromosomes (23 pairs).

Genome

• Definition: The complete set of genetic material in a haploid set of chromosomes.

2.3 Mitosis

• Cell Cycle: Includes interphase (S phase for DNA synthesis) and mitosis, encompassing karyokinesis (nuclear division) and cytokinesis (cell division).

• Stages of Mitosis:

  • Prophase: Chromosomes condense; nuclear envelope breaks down.

  • Prometaphase: Chromosomes move to equatorial plane.

  • Metaphase: Centromeres align on metaphase plate.

  • Anaphase: Sister chromatids separate and move to opposite poles.

  • Telophase: Chromosomes uncoil, nuclear envelope reforms, and cytokinesis occurs.

Regulation of the Cell Cycle

• Checkpoints monitor cell division for errors; cyclins and cdc mutations play critical roles in regulation.

2.4 Meiosis

• Definition: Reduces diploid genetic content to haploid, crucial for sexual reproduction.

• Stages of Meiosis:

  • Meiosis I (Reductional): Homologous chromosomes separate.

  • Meiosis II (Equational): Sister chromatids separate, resulting in haploid gametes.

• Crossing Over: Genetic exchange occurs between homologous chromosomes, increasing genetic diversity.

Comparison of Mitosis and Meiosis

• Mitosis produces identical daughter cells; meiosis creates genetically diverse haploid gametes.

2.5 Gamete Development

• Spermatogenesis: In testes, creates four haploid sperm cells from one primary spermatocyte.

• Oogenesis: In ovaries, usually one ovum is produced from a primary oocyte while other daughter cells become polar bodies.

2.6 Importance of Meiosis

• Essential for the sexual reproduction cycle across various organisms, establishing genetic variation.

2.7 Chromosome Structure Insight

• Chromosomes observable during mitosis and meiosis; coiling and condensation processes identified through electron microscopy.