MICROSCOPE

Week 5, Quiz 5

If magnification is optimized, what else needs to be considered to obtain the best possible image of a specimen?

Resolution

Contrast

If you just wanted to observe what cell shape a particular bacteria is, you could do a ____ stain

Simple

Match the correct type of microscopy to its application.

TRANSMISSION EM: Provides the most highly magnified view of the genetic material of COVID-19.

PHASE CONTRAST: Enables the viewer to quickly determine if microbes from an unstained specimen are motile (able to move) or not

TRANSMISSION EM: Used obtain a highly magnified view of the ribosomes inside a bacterial cell

BRIGHTFIELD: Used to view acid-fast stained bacteria from a tuberculosis patient

Which of these is NOT found in Gram negative bacterial cell walls?

teichoic acids

If you forgot to do the heat-fixing step prior to the Gram stain, predict what you would see:

You would not see anything on your slide

If your microscope has a resolving power of 0.25 microns, you CAN clearly see structures that are ______ microns apart.

0.25

2.5