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Order of Draw for Blood Collection:
Blood Culture Tubes: Used for detecting bacteria/fungi in the blood.
Light Blue Top (Citrate Tubes): Used for coagulation studies (PT, PTT).
Red Top (Serum Tubes): Used for serology and blood chemistry tests (no additives).
Gold/Speckled (SST) Tubes: Serum separator tubes for biochemistry tests; has a gel that separates serum upon centrifugation.
Green Top (Heparin Tubes): Used for plasma tests; contains heparin anticoagulant.
Lavender Top (EDTA Tubes): Used for hematology tests (CBC) and blood bank (crossmatch).
Gray Top (Fluoride Oxalate Tubes): Used for glucose testing and lactate measurements; contains fluoride which inhibits glycolysis.
Tube Color | Additive | Used For |
1⃣ Blood Culture (Yellow/Blue Bottles) | Nutrient broth | Detect infections in blood |
2⃣ Light Blue | Sodium citrate | Clotting tests (PT, PTT, INR) |
3⃣ Red (Plain or Serum Tubes) | No additive or clot activator | Chemistry tests (Electrolytes, Hormones) |
4⃣ Gold/Tiger Top (SST – Serum Separator Tube) | Clot activator + Gel | Similar to red tube but with gel for serum separation |
5⃣ Green | Heparin | Stat chemistry, Cardiac markers |
6⃣ Lavender (Purple) | EDTA | CBC (Complete Blood Count) |
7⃣ Pink | EDTA | Blood bank (Type & Screen, Crossmatch) |
8⃣ Gray | Sodium fluoride | Glucose & Alcohol testing |
NoteStudied by 44 peopleNoteStudied by 2 peopleNoteStudied by 174 peopleNoteStudied by 10 peopleNoteStudied by 67 peopleNoteStudied by 12 people