Biotechnology 11

DNA Technology: Isolation, purification, analyzation, and manipulation of DNA 

Genetic cloning: Know the impact of gene by isolating and replicating it 

• Isolate, replicate, and study genes Cloning Vectors: Ex: bacterial plasmids

Biotechnology: Techniques applied to biological systems for modification 

Genomics: Characterization of whole genomes 

Basic Research: Sturdy gene structure, function, regulation of expression

 Applied Research: Other applications (medical: gene therapy)

Plasmid: circular double stranded DNA distinct from chromosomal DNAthat can replicate independently of the chromosome/s

• Function: vectors for inserting genes 

Recombinant: : DNA fragments from two or more different sources that are joined into a single molecule

Crispr Cas 9

• Crispr: Clustered Regularly Interspaced Short Palindromic Repeats 

  • Has RNA guide complementary to target gene 

  • Cas 9: DNA Endonuclease Cleaves target so new gene can be inserted Recognizes PAM (Protospacer Adjacent Motif) sequence