Chapter 4 (Campbell's Biology in Focus)
Internal Membranes and Functions
- ==Cytosol== is the semifluid, jellylike substance inside cells
- In eukaryotic cells, most of the DNA is found in the ==nucleus==
- In prokaryotic cells, DNA is focused in a region called the ==nucleoid== which is not membrane enclosed
- The interior of either type of these cells is called cytoplasm
- At the boundary of every cell the ==plasma membrane== functions as a selective border that allows oxygen, nutrients, and wastes to service the entire cell
* the plasma membrane consists of a double layer of phospholipids

- ==Microvilli== increase the surface area of an object due to the crevices
\
- The nucleus contains most of the genes in a eukaryotic cell
- The ==nuclear envelope== encloses the nucleus separating its contents from the cytoplasm * the two membranes are each a lipid bilayer associated with proteins

- The nuclear side of the envelope is lined by the ==nuclear lamina==
- DNA is organized into discrete components called ==genes==
- The complex of DNA and proteins making up chromosomes is called ==chromatin==
- A prominent structure in the nondividing nucleus is the ==nucleolus== (plural of nucleoli) which is a mass of densely stained granules and fibers adjoining part of the chromatin
\
- ==Ribosomes== are complexes made of ribosomal (RNA) proteins * They carry out protein synthesis
\
Endomembrane System
- Many of the different membrane-bounded organelles of the eukaryotic cell are part of the ==endomembrane system,== * the endomembrane system includes the * nuclear envelope * endoplasmic reticulum * Golgi apparatus * lysosomes * various kinds of vesicles and vacuoles * plasma membrane.
- The ==endoplasmic reticulum== is a large network of membranes that accounts for more than half of the plasmic membranes in eukaryotic cells
* ==Smooth ER== lacks ribosomes
* ==Rough ER== is studded with ribosomes on the outer surface of the membrane

Functions of Rough ER
- Most secretory proteins are ==glycoproteins==
- ==Transport Vesicles== are vesicles in transit from one part of the cell to another
\
The Golgi Apparatus: the shipping and receiving center
- After leaving the endoplasmic reticulum in transport vesicles, they head to the Golgi Apparatus * The ==Golgi Apparatus== is like a warehouse for receiving, sorting, shipping, and some manufacturing.
\
- A ==lysosome== is a membranous sac of hydrolytic enzymes that many eukaryotic cells use to digest macromolecules
- ==Phagocytosis== is the process of engulfing smaller organisms or food particles * The food vacuole formed through this then fuses with a lysosome


- ==Vacuoles== are large vesicles formed by the endoplasmic reticulum and Golgi Apparatus
- ==Food Vacuoles== are formed by phagocytosis
- Many unicellular protists living in freshwater have ==contractile vacuoles== that pump excess water out of the cell, thereby maintaining a suitable concentration of ions and molecules inside the cell.
- Mature plants generally contain a ==central vacuole==, which develops by the coalescence of smaller vacuoles
\
- The ==endosymbiont theory== states that an early ancestor of eukaryotic cells engulfed an oxygen-using non-photosynthetic prokaryotic cell. Eventually, the engulfed cell formed a relationship with the host cell in which it was enclosed, becoming an endosymbiont.
- The inner folds of a phospholipid bilayer are called a ==cristae==
- The ==cytoskeleton== is a network of fibers extending throughout the cytoplasm
\
- Eukarya have ==microtubules==, hollow rods constructed from a globular protein called tubulin
- In animal cells, microtubules grow out of a ==centrosome==, a region that is often located near the nucleus and is a “microtubule organizing center”
- Within the centrosome is a pair of ==centrioles==, each composed of nine sets of triplet microtubules arranged in a ring * In eukaryotes, a special arrangement of microtubules results in the beating of the flagella and cilia
- The microtubule assembly of a cilium or flagellum is anchored in the cell by the basal body
- Bending involves large motor proteins called dynein
- ==Microfilaments== are thin, solid rods. They are also called actin filaments because they are built from molecules of actin.
- Thousand of actin filaments and thicker filaments of a motor protein called ==myosin== interact to cause a contraction in a muscle cell
- ==Intermediate filaments== are named for their diameter, which is larger than the diameter of microfilaments but smaller than that of microtubules
\