Scientists – Contributions to Microscopy / Cell Theory
Janssens: Contributed to the early development of microscopy.
Hooke: Discovered cells; coined the term "cell" after observing cork.
Van Leeuwenhoek: First to observe live cells; improved lens quality.
Redi: Pioneered experimental techniques; debunked spontaneous generation.
Needham: Supported the idea of spontaneous generation through broth experiments.
Spallanzani: Disproved Needham; emphasized the need for sealing and heating to prevent contamination.
Pasteur: Confirmed that microorganisms cause disease; introduced pasteurization.
Brown: Discovered the nucleus in plant cells.
Schleiden & Schwann: Developed the cell theory, stating all living things are composed of cells.
Cell Theory
All living organisms are composed of one or more cells.
Cells are the basic unit of life.
All cells arise from pre-existing cells.
Parts of the Microscope
Eyepiece, objective lenses, stage, light source, diaphragm, etc.
Microscopy Skills
Calculating Magnification: Multiply the eyepiece and objective lens magnification.
Measuring Field of View (low power): Determine the visible area of the specimen.
Calculating Field of View (high power): Apply the formula to find dimensions at high power.
Calculating Actual Size: Use the field diameter and proportion of the specimen size.
Calculating Scale: Relate size on the image to the actual dimensions.
Unit Conversion: Converting mm to µm (1 mm = 1000 µm).
Advancements in Microscopy
Magnification, Resolution, Contrast: Key factors in improving microscopy.
Light Microscopes vs. Electron Microscopes: Light allows viewing of live specimens; electron provides high resolution but requires specimens to be non-living.
Applications in Industry & Medicine: Includes diagnostics, research, and quality control.
Organelles – Structure/Function
Differences Between Animals and Plants: Plants have cell walls and chloroplasts, while animals do not.
Photosynthesis, Cellular Respiration, Energy Storage
Chemical Reactions: Essential metabolic processes.
Molecules Used by the Cell: Glucose and other carbohydrates used for storage.
Fluid Mosaic Model
Cell Membrane as Phospholipid Bilayer: Comprises hydrophilic heads and hydrophobic tails.
Role in Cell Communication & Transport: Facilitates substance movement in and out of cells.
Transport Mechanisms
Diffusion: Movement from high to low concentration without energy.
Osmosis: Selective movement of water across a semi-permeable membrane.
Facilitated Diffusion: Movement through specific channel proteins.
Active Transport: Movement against concentration gradient requiring energy (ATP).
Endocytosis/Exocytosis: Processes for internalization and secretion of substances.
Tonicity and Its Impact on the Cell
Applications in Industry & Medicine: Importance in therapeutic contexts.
Surface Area to Volume Ratios
Implications of Multicellularity: Efficient transport and resource allocation.
Calculations – What the Numbers Mean: Essential for understanding cell function limitations.
Organization of a Multicellular Organism
Cells, Tissues, Organs, Systems: Hierarchical structure in multicellular organisms.
Structure & Function of Plant Tissues
Dermal Tissue: Upper and lower epidermis protect the plant.
Ground Tissue: Palisade layer (photosynthesis) & spongy mesophyll (gas exchange).
Xylem & Phloem: Transport water and nutrients.
Control Systems in Plants
Opening and Closing the Stoma: Regulation of gas exchange.
Phototropism: Growth towards light; influenced by auxin.
Gravitropism: Response to gravity; roots grow down, shoots grow up.
Transport in Plants
Root Pressure: Pushes water upwards.
Cohesion & Adhesion: Water molecules link and cling to surfaces.
Transpirational Pull: Evaporation creates a vacuum effect for water transport.
Pressure-Flow Theory: Explains nutrient transport in phloem.