Things to go over for final module exam (60 marks -MCQ + Matching)

-Go over definitions for culture, antibiotic susceptibility testing, minimum inhibitory concentration, aerobic, anaerobic, vibrio, coccus, bacillus, spirochetes, microbiology, microorganism, colony, culture, gram positive, gram negative, gram staining, normal flora, colony, catalase test, coagulase test, host, resistant, susceptible, communicable, fastidious bacteria, host, immunoassay, agar, biological safety cabinet, HEPA filter, stock culture, sub-culturing, selective media, indicator medium, enriched medium, enrichment media, inoculation, opportunistic pathogen, medium, antiseptic, aseptic technique, fission, hyphae, mycosis, pathogen, progeny, carrier, septicemia, transport medium, virulent, psychrophiles, zone of inhibition, virion, sterilization, susceptible host, quality control, quality assurance, SOP, O&P

-What is a virus? How do viruses differ from other types of microorganisms?

-What is the name of the microorganism that causes strep throat?

-What is susceptibility testing? What is the name of the media plate used for it? What is the benefit of doing that test?

-Go over the steps for gram staining. What is the primary stain, the mordant, the decolorizer, the counterstain. What are the results? What is gram positive colour? What is gram negative colour? Go over each step as in after you put the crystal violet, then at that stage of time what is the colour for gram negative and gram positive and so on.

-What is the difference between the cell wall of gram positive and gram-negative microorganisms?

-The stain reacts to what part of the microorganisms?

-Go over the Ziehl Neelsen stain. What is it for? What is used as the decolorizing agent?

-What are the characteristics of acid-fast microorganism

-What is the autoclave settings for sterilizing media?

-How should you sterilize media that are heat liable but not sterile?

-What is a selective media, differential media, enriched media, transport media. The different types of media and examples of the names of medias that can be used.

-What are the characteristics for a sheep blood agar? Like when can you use it, do you see hemolysis on it?

-What are the characteristics of a MacConkey agar?

-Routine cultures are incubated at what temperature and atmosphere requirement?

-How should we store commercially prepared culture plates?

-How are petri plates stores in the incubator and fridge? (Remember inverted).

-Where do you label on the petri plates?

-What is opportunistic infection?

-Does sterilization mean killing all microorganism and including SPORES?

-When can an opportunistic microorganism cause infection?

-Psychrophiles grow within what temperature?

-What is an incubator? What are routine incubators set at?

-What temperature incubator is set for cultivation of fungi? What temperature incubator is set for campylobacter?

-Can you list three of the methods discussed for acid-fast bacilli based on the slides. Can you use gram stain for acid-fast bacilli?

-What is immunofluorescent techniques and what is DFA tests?

-Go over the QA for making and staining smears

-When we make a smear why do we heat fix it?

-What is the common microorganism that is responsible for UTI (Urinary Tract Infection)? Is is a gram positive or gram negative and what shape?

-Solid media is used for? What is the percentage of agar added to the media? Agar gets solidified at what temperature? Name some examples of solid media.