CH14
Chapter 14: Arterial Puncture Procedures
Overview of Arterial Puncture
Purpose: Arterial puncture is primarily performed to obtain blood for arterial blood gas (ABG) tests, which are crucial for evaluating respiratory function due to the high oxygen content and consistency of arterial blood.
Disadvantages:
Technically difficult procedure
Potential for more pain and hazards compared to venipuncture
Not typically used for routine blood tests
Objectives of the Chapter
Demonstrate understanding of arterial blood collection terminology, hazards, and complications.
Identify and analyze appropriate arterial puncture sites based on established criteria, along with their advantages and disadvantages.
Describe the ABG collection procedure including patient assessment, equipment necessary, and parameters measured in ABG tests.
Perform and interpret the modified Allen test.
Personnel Involved in Arterial Puncture
Who Can Perform:
Nurses
Medical technologists & technicians
Respiratory therapists
Emergency medical technicians
Level II phlebotomists
Training Required:
Extensive training involving theory, demonstrations, and supervised practice is mandatory.
Site-Selection Criteria for Arterial Punctures
Factors to Consider:
Presence of collateral circulation
Accessibility and size of the artery
Type of surrounding tissue to minimize injury risks during puncture
Absence of inflammation, irritation, or open wounds.
Common Arterial Puncture Sites
1. Radial Artery
Advantages:
Good collateral circulation from radial and ulnar arteries
Easy to locate and palpate
Less chance of hematoma formation
Disadvantages:
Skill required for successful puncture due to small size
Difficult to locate in patients with low blood volume or cardiac output
2. Brachial Artery
Advantages:
Large size makes it easier to palpate and puncture
Preferred for collecting large volumes of blood
Disadvantages:
Deeper location makes palpation harder
Risks of puncturing median nerve and basilic vein
3. Femoral Artery
Key Characteristics:
Largest artery used for arterial puncture, located in the groin
Advantages:
Large and easy to locate
Disadvantages:
Poor collateral circulation
Increased risk of infection and hematoma formation
Other Sites for Arterial Puncture
Infants: Scalp and umbilical arteries
Adults: Dorsalis pedis arteries
Note: Brachial artery is often avoided in infants due to palpation difficulty and poor collateral circulation.
ABG Parameters and Interpretation
Use Cases:
Diagnosis and management of respiratory disorders
Information on oxygenation, ventilation, and acid-base balance
Commonly Measured Analytes:
pH: 7.35–7.45
PaO2: 80–100 mm Hg
PaCO2: 35–45 mm Hg
HCO3: 22–26 mEq/L
O2 Saturation: 97–100%
Base Excess: (−2)–(+ 2) mEq/L
ABG Specimen Collection Procedures
Equipment and Supplies
Personal protective equipment and sterile supplies such as gloves, antiseptics, and syringes
Local anesthetic (optional) and gauze for after-care
Patient Preparation
Proper identification and explanation of the procedure.
Assessment of patient’s steady state and collateral circulation.
Conducting the modified Allen test to assess circulation.
Modified Allen Test Procedure
Patient makes a fist, compressing both radial and ulnar arteries.
Release pressure on the ulnar artery after hand opens.
Assess color return:
Positive if pink flushes in <15 seconds
Negative if no flush occurs.
Radial ABG Collection Steps
Receive and verify test requisition.
Prepare the patient and ensure consent.
Assess and locate the radial artery; optional anesthetic application.
Insert needle at 30-45 degree angle toward pulse, stopping when a flash of blood appears.
Allow syringe to fill, apply pressure, and activate safety feature of the needle.
Label the specimen and dispose of used materials properly.
Monitor the site for complications and apply a bandage.
Hazards and Complications of Arterial Puncture
Common Hazards:
Arteriospasm
Artery damage
Discomfort and infection
Hematoma and thrombus formation
Sampling Errors: Issues such as air bubbles, delay in analysis, and using incorrect anticoagulants can impact results.
Conclusion
Understanding the procedures, anatomy, and complications of arterial puncture is critical for effective practice in phlebotomy and respiratory care. Regular practice and up-to-date knowledge ensure better patient safety and accurate diagnostic results.