All living things are made of cells, which are the basic structural and functional units of life.
All life functions occur in cells, highlighting the cell as the fundamental unit of physiological activity.
All cells come from pre-existing cells through cell division, emphasizing that cells do not spontaneously generate (law of biogenesis).
Cell as the Basic Unit of Life:
Cells are the smallest entities capable of performing life functions.
They contain all the hereditary material (DNA) necessary for directing cellular activities and transmitting information to the next generation.
Life Functions in Cells:
Cells conduct various processes, including metabolism, growth, response to stimuli, and reproduction.
Organelles within the cell perform specific functions necessary for the cell’s survival.
Origin of Cells:
This principle opposes the idea of spontaneous generation.
Cell division involves processes like mitosis and meiosis, ensuring genetic continuity.
Animal cells have centrioles, which are involved in cell division; plant cells do not possess centrioles, relying on other mechanisms for cell division.
Plant cells have cell walls, providing structural support and protection, along with chloroplasts for photosynthesis; animal cells lack both.
Animal cells have smaller vacuoles and vesicles used for storage and transport; plant cells usually have one large central vacuole to maintain cell turgor and store nutrients and waste.
Centrioles:
Animal cells use centrioles to organize microtubules during cell division.
Plant cells utilize other structures like the phragmoplast for cell plate formation during cytokinesis.
Cell Walls and Chloroplasts:
Plant cell walls are primarily made of cellulose, offering rigidity.
Chloroplasts conduct photosynthesis, converting light energy into chemical energy in the form of glucose.
Vacuoles and Storage:
Plant cells' large central vacuole helps maintain turgor pressure, essential for plant rigidity.
Animal cells have numerous smaller vacuoles and vesicles for diverse functions like storage and transport.
Ocular Lens: Focuses the image into the eye, typically providing a magnification of 10x.
Revolving Nose Piece: Selects the objective lens with varying magnifications.
Objective Lenses (Low, Medium, High Power): Provide different levels of magnification (e.g., 4x, 10x, 40x, 100x).
Stage Clip: Holds the slide securely in place on the stage.
Diaphragm: Adjusts the amount of light passing through the specimen, enhancing contrast.
Light Source: Provides illumination to view the specimen.
Stage: Platform that supports the slide.
Arm: Connects the objective lenses and stage to the microscope's base, used for carrying the microscope.
Course/Fine Adjustment: Knobs used to bring the specimen into focus, with the coarse adjustment used for initial focusing and the fine adjustment for detailed clarity.
Base: Supports the microscope, providing stability.
Magnification System:
Objective lenses range from low (4x) to high power (100x), influencing the total magnification.
Total magnification is the product of objective lens magnification and ocular lens magnification (Total = Ocular \; Lens \; Magnification \times Objective \; Lens \; Magnification).
Illumination and Contrast:
The diaphragm controls the amount of light, affecting image contrast and clarity.
Proper illumination is crucial for clear observation of the specimen's details.
Focusing Mechanisms:
Coarse adjustment knob allows large changes in focus, useful for initial adjustments.
Fine adjustment knob provides precise focusing for detailed observation.
1 \; mm = 1000 \; \mu m (Micrometers)
1 \; cm = 10 \; mm
1 \; m = 1000 \; mm
1 \; \mu m = 1000 \; nm (Nanometers)
Total \; Magnification = Ocular \; Lens \; Magnification \times Objective \; Lens \; Magnification
If the ocular lens is 10x and the objective lens is 40x, then:
Total \; Magnification = 10 \times 40 = 400x
FOV{low} \times Magnification{low} = FOV{high} \times Magnification{high}
Purpose:
To determine the field of view at a higher magnification when the field of view at a lower magnification is known.
Example:
If FOV{low} = 2 \; mm, Magnification{low} = 40x, and Magnification{high} = 100x, then: 2 \; mm \times 40 = FOV{high} \times 100
FOV_{high} = \frac{2 \; mm \times 40}{100} = 0.8 \; mm
Mitochondria: Produces ATP through cellular respiration.
Ribosomes: Produce proteins by translating mRNA.
Vacuoles: Store food, water, or wastes; in plant cells, they maintain turgor pressure.
Nucleus: Contains DNA, which controls cell activities.
Chloroplast: Converts solar energy to chemical energy via photosynthesis (in plant cells).
Lysosome: Digests cellular materials, breaking down waste and debris within the cell.
Cell Wall: Protects the cell membrane, providing structural support and shape (in plant cells, made of cellulose; in bacteria, made of peptidoglycan).
Golgi Apparatus: Packages and modifies proteins and lipids for secretion or internal use.
Endoplasmic Reticulum (ER): Intracellular transport network; rough ER has ribosomes for protein synthesis, while smooth ER synthesizes lipids and steroids.
Cell Membrane: Acts as the "gatekeeper" of the cell, regulating the movement of substances in and out.
Energy Production & Synthesis:
Mitochondria use oxygen to produce ATP, the energy currency of the cell.
Ribosomes are found freely in the cytoplasm or attached to the rough ER.
Storage & Genetic Control:
Vacuoles store water, ions, and macromolecules.
The nucleus houses chromatin, which condenses into chromosomes during cell division.
Specialized Organelles:
Chloroplasts contain chlorophyll, which captures light energy.
Lysosomes contain enzymes to break down complex molecules.
Transport & Protection:
The Golgi apparatus modifies and sorts proteins, directing them to specific locations.
The ER facilitates protein folding and lipid synthesis.
Membrane Functions:
The cell membrane is composed of a phospholipid bilayer with embedded proteins.
It controls the passage of ions, nutrients, and waste.
Isotonic: Solution with the same solute concentration as the cell, resulting in no net movement of water.
Hypertonic: Solution with a higher solute concentration than the cell, causing the cell to lose water and shrink (plasmolysis).
Hypotonic: Solution with a lower solute concentration than the cell, causing the cell to absorb water and swell (cytolysis).
Osmosis: Diffusion of water across a selectively permeable membrane from an area of high water concentration to an area of low water concentration.
Diffusion: Movement of particles from an area of high concentration to an area of low concentration until equilibrium is reached.
Semi-permeable: Membrane that allows only certain particles through while restricting others based on size, charge, or chemical properties.
Concentration Gradient: The difference in solute concentration between two areas; particles move down the gradient from high to low concentration.
Equilibrium: State of balance between opposing actions, where the net change is zero.
Tonicity:
Isotonic solutions maintain cell volume and function.
Hypertonic solutions can lead to cell dehydration.
Hypotonic solutions may cause cells to burst.
Membrane Transport:
Osmosis is crucial for maintaining cell turgor and hydration.
Diffusion is vital for nutrient uptake and waste removal.
Selective Permeability:
Semi-permeable membranes ensure cells maintain specific internal environments.
They regulate the passage of essential molecules.
Concentration Dynamics:
The concentration gradient drives passive transport processes.
Equilibrium ensures stability in cellular environments.
Photosynthesis: 6CO2(g) + 6H2O(l) \rightarrow C6H{12}O6(aq) + 6O2(g)
Carbon dioxide + Water yields Glucose + Oxygen
Cellular Respiration: C6H{12}O6(aq) + 6O2(g) \rightarrow 6H2O(l) + 6CO2(g)
Glucose + Oxygen yields Water + Carbon dioxide
Photosynthesis:
This process occurs in chloroplasts.
Light energy is converted into chemical energy.
Cellular Respiration:
This process occurs in mitochondria.
Glucose is broken down to produce ATP, water, and carbon dioxide.
Auxins: Promote cell elongation; responsible for phototropism (growth towards light).
Phototropism:
Auxins accumulate on the shaded side of the plant, promoting cell elongation on that side.
This uneven growth causes the plant to bend towards the light.
Other Effects:
Involved in apical dominance, where the central stem is dominant over lateral buds.
Promotes root development and fruit growth.
Xylem: Transports water and minerals from roots to leaves (dead cells).
Phloem: Transports food (sugars) from leaves to roots (alive cells).
Cohesion: Water molecules stick to each other due to hydrogen bonds.
Adhesion: Water molecules stick to other surfaces (like the walls of xylem).
Xylem Transport:
Relies on transpiration pull, cohesion, and adhesion.
Water moves up the plant through dead xylem cells.
Phloem Transport:
Uses active transport to load sugars into phloem.
Water follows by osmosis, creating pressure that pushes sugars to other parts of the plant.
Osmosis/Diffusion, Adhesion/Cohesion, Transpiration.
Osmosis/Diffusion:
Water moves into root cells via osmosis.
Nutrients move into root cells via diffusion.
Adhesion/Cohesion:
Water molecules stick together and to the walls of the xylem, aiding upward movement.
Transpiration:
Evaporation of water from leaves creates a pulling force that draws water up the xylem; regulates plant temperature, facilitates nutrient transport.
Tropisms: Plant responses to stimuli, which can be positive (growth towards the stimulus) or negative (growth away from the stimulus).
Phototropism:
Response to light.
Stems exhibit positive phototropism, while roots might show negative phototropism.
Gravitropism:
Response to gravity.
Roots exhibit positive gravitropism, while stems exhibit negative gravitropism.
Thigmotropism:
Response to touch.
Seen in climbing plants like vines that wrap around structures for support.