L

Cell basics + neurons

Overview

Basics of Cells and Neurons

Cells are the building blocks of life, and they work together in the nervous system to help us function. The main types of cells to focus on are neurons (nerve cells) and astrocytes (supporting cells).

Structure of Cells

  • Cell Membrane: This is like the skin of the cell, made of a double layer of fats. It controls what goes in and out of the cell. The cell membrane has special areas called lipid rafts that help organize things inside.

  • Ion Channels: These are tiny openings in the membrane that let ions (charged particles) move in and out. There are different types:

    • Sodium/Potassium Pumps: These keep the cell's balance by moving sodium and potassium ions in and out and are super important for neurons.

    • Ligand-Gated Channels: These open when a chemical messenger (like a neurotransmitter) binds to them, changing what ions can enter the cell—this is important for things like addiction.

    • Voltage-Gated Channels: These open when there's a change in voltage (electric charge), helping to send signals quickly along the neuron.

  • G-Protein Coupled Receptors: These are receptors on the cell that, when bound to a molecule, can start other important processes inside the cell, including turning genes on or off.

Neurotransmitters

/

Neurotransmitters are chemicals that neurons use to communicate. They are released at synapses (connections between neurons) and bind to receptors on other neurons, causing changes that can lead to signals being sent along.

  • Vesicles: These are small packages that store neurotransmitters and move them to the cell membrane to be released. This process uses proteins that walk along structures called microtubules, powered by energy from ATP.

Structure of Neurons

  • Parts of a Neuron: A neuron has several main parts:

    • Cell Membrane: The outer layer of the neuron.

    • Cell Body: Contains the nucleus and is where most proteins are made.

    • Dendrites: Branches that receive messages from other neurons; they usually have a resting charge of around -70 mV.

    • Axon Hillock: The beginning of the axon where signals are integrated.

    • Axon: This is a long fiber that carries signals away from the cell body.

    • Axon Terminals: The end parts that send signals to other neurons.

  • Myelin Sheath: This is a protective cover around the axon that helps signals travel faster and prevents the loss of electricity. Gaps in this cover, called Nodes of Ranvier, help boost the signal as it travels.

Sending Signals

The process of sending a signal (action potential) through a neuron happens in steps:

  1. Resting State: The neuron is at rest, and the sodium-potassium pumps keep a balance.

  2. Depolarization: When a stimulus is strong enough, sodium channels open, and sodium enters, making the inside of the cell less negative.

  3. Repolarization: Potassium channels open, and potassium leaves the cell, making the inside negative again.

  4. Hyperpolarization: Sometimes the potassium channels stay open a bit too long, making the inside overly negative for a short time.

  5. Return to Resting Potential: Everything goes back to normal.

How Neurons Work Together

Neurons are influenced by the timing and order of the signals they receive. How they process neurotransmitter signals at the dendrites determines if they will send a signal through the axon. Neurons can communicate in many places, not just from dendrites, adding to how complex our nervous system is.


My notes:

  • people are the outcome of cell cordination

    • cells we care about

    • neurons

    • astrocites

  • Generic cell (parts)

    • cell membrain

      • full of h20 and has a oil layer that doesnt let h2o pass thro, phopholipid bilayer

      • hydrophpic tail

      • hydroloving head

    • lipid rafts

      • things the channles get plugged into, anchors

        • allows things to pass thro cell membrain

        • all stuff foating in raft

        • Holds ion channles

    • Ion channles

      • protien that pass thro the lipid rafts

      • let charged particles back and forth

    • sodium/possium pumps

      • impotant for neruons

        • active transport, using engry

    • gated channle closed sometimes but also opens nerotransmiters open them

    • ligland gated channels

      • important for addiction

      • open gate to let ions in when messanger binds to them

      • ligand means messenger

      • messanger could be dopamine or something like cokecane or sicsofrina meds

      • they appear where synapses are

      • responds to nerotrasmiters

    • Voltage gated ions channles (class of channles)

      • senses two electrical state

      • gate from closed state to open state

      • doesn’t need a messanger just a range of elctrial differncal on both sides of cells (more postive out side than inside causes it to open)

      • on axon

      • stays closed and opens at diff range

    • volatage gated potssiam

      • is for repolaltztion

    • voltage gated calcium

      • in snapse opens calcium to smoosh vessicle into cell memebrane realsing cell membrane

    • G protien coupled recptor

      • have a recption waiting for molecule to bind to it

      • this opens large complex cascade of proteins to do a bunch of tasks

      • are dependent on internal state of cell being in certain state

      • can open up ion channle or can effct what DNA gets transcribed and turned into protein

      • binding site for transmiter and may do many things

  • where do recptors, enzymes and proteins come from

    • necleus membrane has DNA inside it

    • so based on the sate of rest of cell the double helex of DNA get unattched and make opposite copy in RNA

    • RNA gets sent out to determe order of amino acids to make strands of protein

    • protien is the formation of everything

    • Zinc finger proteins- attach to DNA so that certian genes can be turned into protiens and other parts do not (the expresion is very locailised, very regionally specific and tied to certian functions)

  • Protien are evrything

    • amino acids in a row are as meaningfull as bunch of random letters in a row

    • amino acids get turned into words and then more meaningful sentances and then get combined into even more soecific until useful/has disired effect

    • complexity in maping DNA to functionlal protiens

  • Variety in protein assmbly leads to variety of function

    • depening on sub units that make up ligated gated channel, but depending on assembly changes what activates the channel to let cholride thro

    • ligengated channel in ex

    • so basically proteins are made slighly differntly to provide slighlt diff function

    • like diff emphasis on diff words in a sentance

  • vessicles

    • pakage made of phospholipid bilayer and get shipeed to cell membrane from golgi appartus

    • could have nerotansmiters to cross snyaptic cleft, enzymes, or lots of other things

  • cellular trabnsport

    • kinisn protiens walk down microtuble by ATP to where they need to be

  • PARTS OF NEURON

    • cell membrane, defines what is the neuron

    • cell body, where protiens are generated

    • dendrites, where info is summerized via chemical electrical

      • if you plug elsctrode in dendrite its - 70 mV

      • nerotransmiters (messenger) bind to lingated ion channle recrptors

      • lets in sodium which causes small excitory potential

      • the total amount of sodium let in over space and time is what triggers action potential

        • every time the dendrite lets a little sodium in causes a tiney excitory response

        • the sodium doedn’t just stay put it moves to disspate across electrical and concentraition gradent, which brings its pos charge to other parts of cell

        • so the lowest dendrite branch that is closest to recording site has a higher concentraition of charge but the further the branch is rom the recording site the more diluted it is

    • axion hillock begining of axon

    • axon, action potential is tranfered to axon terminal

    • semaptic end blubs, where info is realsed to next cell

    • geial cells, glue of nervous system and mylanate the axon

    • mylin sheath, procetion of axon and allows info to process quickly

    • gaps in between mylin sheath are premiable

  • Membraine poteintal

    • as you move elctrode closer to cell there is no diff but once you go thro cell membrane it goes down to neg 70 mV

    • this means that there is action poteial in membraine, which is maintained by sodium potaium pumps

    • Generaly excitory

      • Ca+2 or Na+

    • Generaly inhibitory

      • K+ (pos thing going out of cell causes neg change inside) or Cl-

  • Intro to nurons

    • steps of chemical messaging

      • reception

      • intagration

      • electric switch

      • signal progation

      • signal trasduction of chemical messangers

    • Dendrites- order and timing of nerutransmites matter

    • if you get a bunch of nerotransmiter responses at bottom better reation

    • Axon Hillock

      • Gated ion channles switch electeric and fires action potential

      • if up to threshold we get same set of steps:

        • resting, basic cell sate

        • depolarazation, postive charge changes differnachel so then the postive ions come in, it then gors under the -50 as it takes postive ions

        • repolarazation, potasium voltage gated channels take cell and make sure it goes to -50

        • Hyperpolarization, channles stay open too long and dips below neg 70

        • resting, return back to resting potential

      • Myelin sheath

        • insulates the action potienal

        • makes it travel faster

        • stops ions from escaping had sodium move through very quickly till it hits the next patch of tissue and depolarizes it

      • Node of ranvier

        • influx of sodium travels down mylinsheath and the depolarozes, happens over and over and over again

      • Sypnaptic terminal

        • travels down side of this to volatage gated chanel

        • calicum binds vessicles to neruotransmiter to cellular membrane

      • Snyapes aren’t exclusive with dendrites

        • they can be lots of places

        • axon holloic (if there it causes a response faster)

        • right at the start of another synapse (if there trigger nerotranmiter realses faster without travling down axon, or it would cause an ecetrical or chemical gradient)

        • gap juniction- direct contact between cells that are formed so that they can share electrical charge

      • chemical to electrical to chemical

      • post synaptic potentals at the dendrites intergated at the soma

    • Presnaptic is realsing NT

    • Postsnyaptic is receving NT

    • frequancy of action poteintals not the magnitude is how info is encoded.