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Biology: Chap. 10
Biology: Chap. 10
Protein Synthesis: Translation
Overview
Translation occurs after initiation.
The tRNA is associated with the start codon, and the large subunit has come.
The p site fits in this tRNA amino acid complex, containing the first amino acid.
Elongation Process
Codon Recognition
: A tRNA comes and associates with the codon.
The A site is empty, awaiting the next tRNA.
Example: If the codon is UUU, a tRNA with the anticodon AAA will associate with it.
This tRNA carries the amino acid phenylalanine.
Peptide Bond Formation
: The ribosome breaks the covalent bond between the tRNA and the first amino acid.
It catalyzes the formation of a covalent bond between the two amino acids.
Example: Methionine is attached to phenylalanine, forming the start of the protein.
Translocation
: The ribosome moves down the messenger RNA by one codon (three nucleotides).
The first tRNA moves to the E site (exit site).
The second tRNA moves to the P site, carrying the first two amino acids (e.g., phenylalanine and methionine).
The tRNAs in the E site exits; in eukaryotes, tRNAs are potentially one-time use only.
This process repeats with a new tRNA entering the A site.
Example: A third tRNA with anticodon ACG comes in. The corresponding codon being UGC codes for the amino acid cysteine.
The bond is broken, and a covalent bond forms between the existing chain and the new amino acid, then translocation occurs again.
Components and Their Roles
Messenger RNA (mRNA)
: Contains codons, which are triplets of nucleotides.
Serves as the template for protein synthesis.
Ribosome
: Facilitates the process; has a small and large subunit.
E site: Exit site for tRNA.
P site: Holds the tRNA carrying the growing polypeptide chain.
A site: Entry site for the next tRNA.
tRNA
: Transfers amino acids to the ribosome corresponding to each codon.
Codons and the Genetic Code
Codons are mRNA sequences. The chart provided typically shows mRNA codons.
The coding sequence of DNA matches the mRNA sequence but with T instead of U.
Example:
If the DNA non-coding sequence is ATC AAA, the coding sequence is ATG TTT.
The genetic code dictates which codons correspond to which amino acids.
Translocation Defined
Codon recognition: new tRNA enters A site.
Peptide bond formation: bond is formed.
Translocation: Ribosome moves down a notch.
Biological Significance
Proteins are polymers of amino acids; the order of amino acids determines protein shape and function.
Example: Methionine, phenylalanine, cysteine, and lysine are amino acids.
The messenger RNA sequence determines the order of amino acids in the protein.
The mRNA sequence is based on the DNA sequence of the gene.
Termination
Translation continues until the ribosome reaches one of three stop codons: UAA, UAG, and UGA.
These codons do not code for an amino acid.
A special protein inserts itself into the A site, causing the ribosome to dissociate and the protein to be released.
In eukaryotes, mRNA is generally for one-time use only.
Genetic Code Universality and Mutations
Genetic code is universal across nearly all living things.
Implications for genetic modifications.
Bacteria can make human proteins because they recognize the same genetic code.
Mutations
Changes in DNA sequence can occur in various locations.
Location of Mutation and Effect
Between Genes
: Changes in these regions typically have no effect.
Within a Gene
: Changes in UTRs (untranslated regions) or introns have no effect on protein structure.
The vast majority of DNA changes occur in these regions, with no impact on biology.
Within Exons (Coding Regions)
:
Silent Mutation
: A change in the DNA sequence does not alter the amino acid coded for.
Example: UGC and UGU both code for cysteine.
No effect on protein shape or function.
Missense Mutation
: A change in the DNA sequence alters the amino acid coded for.
Example: Changing ACG to ACC changes the codon from UGC (cysteine) to UGG (tryptophan).
Potential to change the organism's biology, leading to variable effects: no impact, protein breaking, or altered protein function.
Nonsense Mutation
: A change in the DNA sequence results in a stop codon.
Example: Changing ACG to ACU changes the codon to UGA (stop codon).
Results in a broken or truncated protein with loss of function.
Indels/Frameshift Mutation
: Insertion or deletion of a nucleotide shifts the reading frame.
Results in a completely different amino acid sequence after the mutation, often leading to non-functional proteins.
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Chapter 36: Current Issues in Macro Theory and Policy
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Parts Of An Microscope
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Studied by 28 people
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Primary ciliary dyskinaesia
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Studied by 9 people
5.0
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AP World History Unit 7 - Lesson 7.5
Note
Studied by 173 people
5.0
(2)
Ch 28 - The Economic and Sustainable Development
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Studied by 41 people
5.0
(1)
Distribution (Place) Decisions
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Studied by 3 people
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