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Chapter 17: Microorganisms and their Applications in Biotechnology

Viruses

  • Not considered as cells - no protoplasm.

  • Live on host cell.

  • When they are living on host cell they are considered as living thing.

  • They do not feed, respire, excrete, grow or reproduce - but; inside a host cell, virus can reproduce or replicate.

  • Viral diseases and our defence system:

    • cold, influenza, chickenpox, dengue, haemorrhagic fever, herpes and AIDS.

    • can only be destroyed by antibodies produced by our WBCs.

Bacteria

  • Living cells.

  • Larger than viruses.

  • Non-motile.

  • Posses hair like threads called flagella.

  • Three general types:

    • Cocci - spherical shaped

    • Bacilli - rod shaped

    • Spirilla - spiral shaped

  • Bacteria can be:

    • Saprophytic - feeding on decaying organic material.

    • Parasitic - causing diseases in plants and animals.

    • Autotrophic - able to manufacture food using energy from the sun or inorganic material.

Fungi

  • Live as saprophytes.

  • Unicellular e.g yeast or multicellular e.g bread mould.

  • Parasitic fungi live on the living tissue of their hosts.

Role of microorganisms in decomposition

  • Decomposers in nature

    • Decomposers feed on dead and decaying organisms and their faeces.

    • They secrete enzymes which break down complex organic compounds to simple organic compounds.

    • They absorb small amount of energy and nutrients; most of the energy is lost as heat and the remaining nutrients are released into surrounding.

    • Inorganic compounds compounds release gases like CO2, hydrogen sulphide and water vapour.

    • Help maintain life on earth.

  • Decomposers in sewage:

    • Decomposed by saprophytic microorganisms.

    • Bacteria in sewage secrete an enzyme to digest solid organic matter into soluble harmless substances.

Biotechnology

  • Fermentation

    • Yoghurt making

      • By fermenting milk and using lactobacillus bulgaricus (bacteria).

      • In the absence of O2 bacteria respires anaerobically and converts lactose to lactic acid .

      • This acid curdles the milk producing yogurt, it can later be flavored or sweetened.

    • Cheese making

      • Use both bacteria and fungi.

      • Lactobacillus is used to ferment milk sugar to lactic acid. The mixture curdles milk protein.

      • Curdled protein together with the fats in the milk, is removed and acted upon by a mixture of bacteria and fungi to produce cheese.

      • By different temperatures and different variation of bacteria and fungi different kind of cheese is produced.

    • Production of alcohol

      • Yeast is used for brewing.

      • Yeast is mixed with sugar.

      • Yeast starts to respire anaerobically to produce alcohol and carbon dioxide from sugar.

Antibiotics

  • Complex substances produced by microorganisms.

  • Used in treatment of bacterial infections.

  • Commercial production.

  • Penicillin production.

    Single-cell protein

    • eg. bacteria and yeast.

    • Help produce animal feed and human food.

Industrial Biotechnology

  • Fermenters

    • Designed to keep its inside environment favourable for the desired biological process.

    • Designed for aerobic and anaerobic processes both.

    • Important features:

      • Cooling system

        • Removal of heat during microbial activities.

      • aeration system

        • For proper mixing ad adequate aeration.

Chapter 17: Microorganisms and their Applications in Biotechnology

Viruses

  • Not considered as cells - no protoplasm.

  • Live on host cell.

  • When they are living on host cell they are considered as living thing.

  • They do not feed, respire, excrete, grow or reproduce - but; inside a host cell, virus can reproduce or replicate.

  • Viral diseases and our defence system:

    • cold, influenza, chickenpox, dengue, haemorrhagic fever, herpes and AIDS.

    • can only be destroyed by antibodies produced by our WBCs.

Bacteria

  • Living cells.

  • Larger than viruses.

  • Non-motile.

  • Posses hair like threads called flagella.

  • Three general types:

    • Cocci - spherical shaped

    • Bacilli - rod shaped

    • Spirilla - spiral shaped

  • Bacteria can be:

    • Saprophytic - feeding on decaying organic material.

    • Parasitic - causing diseases in plants and animals.

    • Autotrophic - able to manufacture food using energy from the sun or inorganic material.

Fungi

  • Live as saprophytes.

  • Unicellular e.g yeast or multicellular e.g bread mould.

  • Parasitic fungi live on the living tissue of their hosts.

Role of microorganisms in decomposition

  • Decomposers in nature

    • Decomposers feed on dead and decaying organisms and their faeces.

    • They secrete enzymes which break down complex organic compounds to simple organic compounds.

    • They absorb small amount of energy and nutrients; most of the energy is lost as heat and the remaining nutrients are released into surrounding.

    • Inorganic compounds compounds release gases like CO2, hydrogen sulphide and water vapour.

    • Help maintain life on earth.

  • Decomposers in sewage:

    • Decomposed by saprophytic microorganisms.

    • Bacteria in sewage secrete an enzyme to digest solid organic matter into soluble harmless substances.

Biotechnology

  • Fermentation

    • Yoghurt making

      • By fermenting milk and using lactobacillus bulgaricus (bacteria).

      • In the absence of O2 bacteria respires anaerobically and converts lactose to lactic acid .

      • This acid curdles the milk producing yogurt, it can later be flavored or sweetened.

    • Cheese making

      • Use both bacteria and fungi.

      • Lactobacillus is used to ferment milk sugar to lactic acid. The mixture curdles milk protein.

      • Curdled protein together with the fats in the milk, is removed and acted upon by a mixture of bacteria and fungi to produce cheese.

      • By different temperatures and different variation of bacteria and fungi different kind of cheese is produced.

    • Production of alcohol

      • Yeast is used for brewing.

      • Yeast is mixed with sugar.

      • Yeast starts to respire anaerobically to produce alcohol and carbon dioxide from sugar.

Antibiotics

  • Complex substances produced by microorganisms.

  • Used in treatment of bacterial infections.

  • Commercial production.

  • Penicillin production.

    Single-cell protein

    • eg. bacteria and yeast.

    • Help produce animal feed and human food.

Industrial Biotechnology

  • Fermenters

    • Designed to keep its inside environment favourable for the desired biological process.

    • Designed for aerobic and anaerobic processes both.

    • Important features:

      • Cooling system

        • Removal of heat during microbial activities.

      • aeration system

        • For proper mixing ad adequate aeration.