-Is media a single or plural form? What is the media? What are the uses of media?
-All media must be _________________before use and what does it destroy?
-Liquid media (broth) is used to culture? What are the different nutrient components? What are some examples?
-Solid media is used for? What is the percentage of agar added to the media? Agar gets solidified at what temperature? Name some examples of solid media.
-An afar gel is formed when? – the three points (on slide 6 of 42)
-What is semisolid media and how much percentage of agar does it contain. Name some examples.
-What are the requirements considered when choosing a suitable medium for the group of microorganisms.
-What is the type of water used for preparing the media? Why can you not use hard tap water?
-What is autotroph and heterotroph
-Most bacteria grow best around what _______environment in terms of pH
-What is used as an energy source in media?
-What does general purpose media usually contain a source of? Name some general-purpose media.
-What are examples of anaerobic media? What is laked blood agar?
-What is selective media and name some examples of selective media.
-Review steps to preparation of culture media.
-When we are preparing media such as nutrient agar and nutrient broth, why do we use 5% tartaric acid? As in what is the purpose for it?
-We use filtration as a way of sterilization for what type of organisms?
-Whole blood is added to molten agar to make blood agar at what temperature?
-What is stock culture?
-What is sub-culturing?
-Review quality control section.