Midterm Review
Light-dependent Reactions
Occur in the thylakoid membrane of chloroplasts
Require light energy and water
Produce ATP, NADPH, and oxygen as byproduct
ATP and NADPH are used in the Calvin cycle
Electron Transport Chain
Light first hits PSII & excites electron pair
PSII splits water and releases an electron and a hydrogen proton, oxygen gas is released as a byproduct
The excited electron moves on
The energy from e- allows the cytochrome to pump hydrogen protons across the thylakoid membrane to the lumen
Light hits PSI and re-excites electrons
e- move on & helps produce NADPH
Meanwhile, H+ goes across the thylakoid membrane through ATP Synthase (produces ATP)
ATP and NADH move to Calvin Cycle
Calvin Cycle (Light-independent Reactions)
Occur in the stroma of chloroplasts
Does not require light directly
Use ATP, NADPH, and carbon dioxide
Produce glucose and regenerate starting molecules (NADPH → NADP+) (ATP → ADP)
Carbon fixation
Carbon dioxide enters & combines with 5-carbon compound already present.
A 3-carbon compound continues to cycle (every 6 carbon dioxides that enters, 12 3-carbon compounds are produced)
Reduction
Mid cycle, 2 of 12 3-carbon compounds are removed to become the building blocks for sugar production (requires ATP and NADPH)
Regeneration
Remaining 10 molecules convert back to 6 5-carbon molecules
Cell: Basic unit of life
Organelles: Specialized structures within a cell
Prokaryotic Cells
Lack a nucleus
Simple structure
Examples: bacteria, archaea
Eukaryotic Cells
Have a nucleus
Complex structure
Examples: animals, plants, fungi
Characteristics of Living Things
Reproduce (sexual & asexual)
Biological inheritance is carried in DNA
Can grow & develop
Obtain materials & energy (metabolism)
Respond to environment (stimulus)
Homeostasis
Evolve
Cell Wall
Provides shape and protection
Composed of bacteria
porous
Cell Membrane
Controls entry and exit of substances
Phospholipid bilayer with embedded proteins
Cytoplasm
Gel-like substance inside the cell
Contains enzymes and cellular structures
Nucleoid
Region where DNA is located
Not enclosed by a membrane
Ribosomes
Site of protein synthesis (follow DNA instructions)
Composed of RNA and proteins
Located on rough ER or in cytoplasm
Nucleus
Contains DNA (in chromosomes in the form of chromatin)
Chromatin- a complex of DNA
Controls cell activities
Nucleolus- where ribosome assembly starts
Nuclear Membrane surrounds nucleus (porous)
Cell Membrane
Selectively permeable barrier
Maintains cell shape
phospholipid bilayer- forms barrier to outside substances
Cytoplasm
Fluid-filled region between nucleus and cell membrane
Contains organelles
Endoplasmic Reticulum (ER)
Rough ER: Synthesis of proteins
Smooth ER: Lipid synthesis, detoxification of drugs
Golgi Apparatus
Modifies, sorts, and packages proteins
Forms vesicles for transport (transports proteins)
Mitochondria
Produces energy (ATP) through cellular respiration
Double membrane structure
Chloroplasts
Found in plant cells
Site of photosynthesis
Contains chlorophyll (absorbs sunlight)
Lysosomes
Contains digestive enzymes
Breaks down waste materials
Filled with enzymes
Vacuoles
Stores water, nutrients, and waste
Large central vacuole in plant cells
pressure makes plant cell ridged (so can support flowers)
Contractile Vacuole- pumps water out of cell
Cytoskeleton
Provides structural support and cell movement
Made of microtubules, microfil
Microfilaments
Thread-like structures made up of actin (a protein)
Helps cell move
Microtubles
Hollow structures made of tubulins (a protein)
Helps with cell division
Builds cilia and flagella
Aerobic Respiration
Glycolysis
Krebs Cycle (Citric Acid Cycle)
Electron Transport Chain
Anaerobic Respiration
Lactic Acid Fermentation
Alcoholic Fermentation
Sugar Breaking
Two ATP invested in cycle (at the end has a net gain of 2)
Glucose is broken into 2 molecules of pyruvic acid
4 e- are passed to NAD+ which makes NADH (takes e- to ETC)
Pyruvic acid (from glycolysis) broken into carbon dioxide
1 Carbon dioxide is released
Rest of pyruvic acid joins acetic acid which joins co-enzyme A to form Acetyl CoA
Acetyl CoA gives 2 carbon Acetyl groups to cycle to 4-carbon molecule already present (which produces a 6-carbon molecule called Citric Acid)
Releases carbon dioxide
Produces ATP, NADH, and FADH2 (goes to ETC)
Uses e- to synthesize ATP from ADP
At the end, an enzyme combines e- with a hydrogen & oxygen to form water
e- send H+ across the membrane- the force makes ATP Synthase spin
During each rotation the enzyme attaches a phosphate group making ADP → ATP
Lactic Acid Fermentation
Pyruvic acid + NADH → Lactic acid + NAD+
Muscle cells during intense exercise
Alcoholic Fermentation
Yeast cells
Pyruvic acid + NADH → Alcohol + Carbon Dioxide + NAD+
Production of ethanol and carbon dioxide
Exercise <90 seconds
Cellular respiration only way to produce enough ATP
stores energy in muscle cells & tissues
After 15-20 mins, breaks down fats
Glycogenolysis
Breakdown of glycogen to glucose
Glucose uptake
Transport of glucose into cells
ATP hydrolysis
Conversion of ATP to ADP + Pi for immediate energy release
Note: This mind map provides a simplified overview of the topics. Further details and connections can be explored within each branch and sub-branch.
Carbohydrates
Carbon, hydrogen, oxygen (1:2:1)
Structural support & protection
Immediate energy
Monomer = monosaccharides (ex. glucose, fructose)
Hydrophilic
Proteins
Monomer = amino Acids
Carbon, hydrogen, oxygen, nitrogen
Peptide bonding is chemical bond between molecules (covalent)
Control rate of reaction (catalyst)
Regulate processes
Form important structures
Fight disease
Enable cell interactions
Hydrophilic
Lipids
Carbon, hydrogen, oxygen
Hydrophobic
Store energy (long term)
Form parts of membranes
Water proof (waxy covering)
No specific monomer
Glycerol (docking molecule)
Combines with fatty acids to form lipids
Nucleic Acids
Carbon, hydrogen, oxygen, nitrogen, phosphorus
Monomer = nucleotides
2 kinds:
RNA
DNA
Can be both, hydrophilic and hydrophobic
Water
Polar
Forms hydrogen bonds (weaker then covalent & ionic)
Cohesion
Surface tension
Attraction between molecules of same substance
Adhesion
‘Sticking together’ of substances
Capillary action = if adhesion causes something to go against gravity
Attraction between molecules of different substances
Heat Capacity
Amount of energy needed to raise waters temperature by making molecules move faster
Protects organisms from drastic temperature changes
Solution
All components are equally distributed
Water polarity allows it to dissolve ionic compound & other polar molecules
When certain amount of water dissolves all of the solute it can, solution is saturated
Suspensions
Materials that don’t dissolve in water but separate in small pieces that don’t settle
Movement of water molecules keeps them suspended (ex. blood)
Mixture
Material composed of 2 or more elements that are physically mixed but not chemically combined
pH scale
Ranges from 0-14
At pH of 7, OH- and H+ ions are equal
Solutions with pH above 7 are ‘basic’ (have more OH-)
Solutions with pH below 7 are ‘acidic’ (have more H+)
Factor of 10 (ex. a solution of pH 4 has 10x as many H+ ions as solution with a pH of 5)
Bases
basic solutions
strong bases are 11-14 pH
Buffers
Help control pH levels
Internal pH of most cells is 6.5 - 7.5
Active Site
Where substrate binds to enzyme
Substrate
Molecule that binds to enzyme
What enzyme breaks down OR builds up
For reaction to occur- reactants must collide with each other with sufficient energy that existing bonds will be broken so new ones can form
Enzyme Function
Catalysis
if activation energy is too high or too slow, catalysis speed up reactions
Factors Affecting Enzyme Activity
Temperature
pH
Substrate Concentration
Enzyme Concentration
Light-dependent Reactions
Occur in the thylakoid membrane of chloroplasts
Require light energy and water
Produce ATP, NADPH, and oxygen as byproduct
ATP and NADPH are used in the Calvin cycle
Electron Transport Chain
Light first hits PSII & excites electron pair
PSII splits water and releases an electron and a hydrogen proton, oxygen gas is released as a byproduct
The excited electron moves on
The energy from e- allows the cytochrome to pump hydrogen protons across the thylakoid membrane to the lumen
Light hits PSI and re-excites electrons
e- move on & helps produce NADPH
Meanwhile, H+ goes across the thylakoid membrane through ATP Synthase (produces ATP)
ATP and NADH move to Calvin Cycle
Calvin Cycle (Light-independent Reactions)
Occur in the stroma of chloroplasts
Does not require light directly
Use ATP, NADPH, and carbon dioxide
Produce glucose and regenerate starting molecules (NADPH → NADP+) (ATP → ADP)
Carbon fixation
Carbon dioxide enters & combines with 5-carbon compound already present.
A 3-carbon compound continues to cycle (every 6 carbon dioxides that enters, 12 3-carbon compounds are produced)
Reduction
Mid cycle, 2 of 12 3-carbon compounds are removed to become the building blocks for sugar production (requires ATP and NADPH)
Regeneration
Remaining 10 molecules convert back to 6 5-carbon molecules
Cell: Basic unit of life
Organelles: Specialized structures within a cell
Prokaryotic Cells
Lack a nucleus
Simple structure
Examples: bacteria, archaea
Eukaryotic Cells
Have a nucleus
Complex structure
Examples: animals, plants, fungi
Characteristics of Living Things
Reproduce (sexual & asexual)
Biological inheritance is carried in DNA
Can grow & develop
Obtain materials & energy (metabolism)
Respond to environment (stimulus)
Homeostasis
Evolve
Cell Wall
Provides shape and protection
Composed of bacteria
porous
Cell Membrane
Controls entry and exit of substances
Phospholipid bilayer with embedded proteins
Cytoplasm
Gel-like substance inside the cell
Contains enzymes and cellular structures
Nucleoid
Region where DNA is located
Not enclosed by a membrane
Ribosomes
Site of protein synthesis (follow DNA instructions)
Composed of RNA and proteins
Located on rough ER or in cytoplasm
Nucleus
Contains DNA (in chromosomes in the form of chromatin)
Chromatin- a complex of DNA
Controls cell activities
Nucleolus- where ribosome assembly starts
Nuclear Membrane surrounds nucleus (porous)
Cell Membrane
Selectively permeable barrier
Maintains cell shape
phospholipid bilayer- forms barrier to outside substances
Cytoplasm
Fluid-filled region between nucleus and cell membrane
Contains organelles
Endoplasmic Reticulum (ER)
Rough ER: Synthesis of proteins
Smooth ER: Lipid synthesis, detoxification of drugs
Golgi Apparatus
Modifies, sorts, and packages proteins
Forms vesicles for transport (transports proteins)
Mitochondria
Produces energy (ATP) through cellular respiration
Double membrane structure
Chloroplasts
Found in plant cells
Site of photosynthesis
Contains chlorophyll (absorbs sunlight)
Lysosomes
Contains digestive enzymes
Breaks down waste materials
Filled with enzymes
Vacuoles
Stores water, nutrients, and waste
Large central vacuole in plant cells
pressure makes plant cell ridged (so can support flowers)
Contractile Vacuole- pumps water out of cell
Cytoskeleton
Provides structural support and cell movement
Made of microtubules, microfil
Microfilaments
Thread-like structures made up of actin (a protein)
Helps cell move
Microtubles
Hollow structures made of tubulins (a protein)
Helps with cell division
Builds cilia and flagella
Aerobic Respiration
Glycolysis
Krebs Cycle (Citric Acid Cycle)
Electron Transport Chain
Anaerobic Respiration
Lactic Acid Fermentation
Alcoholic Fermentation
Sugar Breaking
Two ATP invested in cycle (at the end has a net gain of 2)
Glucose is broken into 2 molecules of pyruvic acid
4 e- are passed to NAD+ which makes NADH (takes e- to ETC)
Pyruvic acid (from glycolysis) broken into carbon dioxide
1 Carbon dioxide is released
Rest of pyruvic acid joins acetic acid which joins co-enzyme A to form Acetyl CoA
Acetyl CoA gives 2 carbon Acetyl groups to cycle to 4-carbon molecule already present (which produces a 6-carbon molecule called Citric Acid)
Releases carbon dioxide
Produces ATP, NADH, and FADH2 (goes to ETC)
Uses e- to synthesize ATP from ADP
At the end, an enzyme combines e- with a hydrogen & oxygen to form water
e- send H+ across the membrane- the force makes ATP Synthase spin
During each rotation the enzyme attaches a phosphate group making ADP → ATP
Lactic Acid Fermentation
Pyruvic acid + NADH → Lactic acid + NAD+
Muscle cells during intense exercise
Alcoholic Fermentation
Yeast cells
Pyruvic acid + NADH → Alcohol + Carbon Dioxide + NAD+
Production of ethanol and carbon dioxide
Exercise <90 seconds
Cellular respiration only way to produce enough ATP
stores energy in muscle cells & tissues
After 15-20 mins, breaks down fats
Glycogenolysis
Breakdown of glycogen to glucose
Glucose uptake
Transport of glucose into cells
ATP hydrolysis
Conversion of ATP to ADP + Pi for immediate energy release
Note: This mind map provides a simplified overview of the topics. Further details and connections can be explored within each branch and sub-branch.
Carbohydrates
Carbon, hydrogen, oxygen (1:2:1)
Structural support & protection
Immediate energy
Monomer = monosaccharides (ex. glucose, fructose)
Hydrophilic
Proteins
Monomer = amino Acids
Carbon, hydrogen, oxygen, nitrogen
Peptide bonding is chemical bond between molecules (covalent)
Control rate of reaction (catalyst)
Regulate processes
Form important structures
Fight disease
Enable cell interactions
Hydrophilic
Lipids
Carbon, hydrogen, oxygen
Hydrophobic
Store energy (long term)
Form parts of membranes
Water proof (waxy covering)
No specific monomer
Glycerol (docking molecule)
Combines with fatty acids to form lipids
Nucleic Acids
Carbon, hydrogen, oxygen, nitrogen, phosphorus
Monomer = nucleotides
2 kinds:
RNA
DNA
Can be both, hydrophilic and hydrophobic
Water
Polar
Forms hydrogen bonds (weaker then covalent & ionic)
Cohesion
Surface tension
Attraction between molecules of same substance
Adhesion
‘Sticking together’ of substances
Capillary action = if adhesion causes something to go against gravity
Attraction between molecules of different substances
Heat Capacity
Amount of energy needed to raise waters temperature by making molecules move faster
Protects organisms from drastic temperature changes
Solution
All components are equally distributed
Water polarity allows it to dissolve ionic compound & other polar molecules
When certain amount of water dissolves all of the solute it can, solution is saturated
Suspensions
Materials that don’t dissolve in water but separate in small pieces that don’t settle
Movement of water molecules keeps them suspended (ex. blood)
Mixture
Material composed of 2 or more elements that are physically mixed but not chemically combined
pH scale
Ranges from 0-14
At pH of 7, OH- and H+ ions are equal
Solutions with pH above 7 are ‘basic’ (have more OH-)
Solutions with pH below 7 are ‘acidic’ (have more H+)
Factor of 10 (ex. a solution of pH 4 has 10x as many H+ ions as solution with a pH of 5)
Bases
basic solutions
strong bases are 11-14 pH
Buffers
Help control pH levels
Internal pH of most cells is 6.5 - 7.5
Active Site
Where substrate binds to enzyme
Substrate
Molecule that binds to enzyme
What enzyme breaks down OR builds up
For reaction to occur- reactants must collide with each other with sufficient energy that existing bonds will be broken so new ones can form
Enzyme Function
Catalysis
if activation energy is too high or too slow, catalysis speed up reactions
Factors Affecting Enzyme Activity
Temperature
pH
Substrate Concentration
Enzyme Concentration