Concise Summary of Human XL Oncology Array Kit Instructions

Product Overview

• Human XL Oncology Array Kit designed for research use to determine relative levels of cancer-related proteins.
• Contains 84 capture antibodies specific to human cancer proteins.
• Enables simultaneous detection without the need for multiple ELISAs or Western blots.

Assay Principle

• Proteins from various samples (cell lysates, serum, saliva, etc.) are captured on membranes with antibodies.
• Detected via a biotinylated cocktail and chemiluminescent reagents, creating a signal indicative of protein levels.

Technical Hints

• Only for research; not diagnostic.
• Use within expiration date; avoid contamination.
• Follow precise handling and washing protocols to ensure assay integrity.

Storage Conditions

• Store unopened kit at 2-8 °C; opened components have specified storage conditions.

Required Supplies

• Pipettes, PBS, lysis buffer, various reagents and equipment for sample handling.

Sample Collection and Preparation

• Guidelines for preparing various sample types (cell culture supernatants, serum, etc.) to maintain integrity.

Reagent Preparation

• Step-by-step instructions for preparing buffers and reagents prior to assay.

Protocol Steps

• Series of steps detailing the assay process, from blocking membranes to detection and signal generation.
• Includes thorough wash steps crucial for reducing background and enhancing signal clarity.

Data Analysis

• Guidelines for analyzing signals captured on X-ray film, including template setup and pixel density assessment.

Profiling Proteins

• Ability to profile proteins across various types like cell culture supernates, body fluids, and tissue lysates with specific sample amounts outlined.

Appendix

• Comprehensive listing of analytes, their corresponding coordinates on the array, and related gene identification numbers.