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Concise Summary of Human XL Oncology Array Kit Instructions

  • Product Overview

    • Human XL Oncology Array Kit designed for research use to determine relative levels of cancer-related proteins.
    • Contains 84 capture antibodies specific to human cancer proteins.
    • Enables simultaneous detection without the need for multiple ELISAs or Western blots.

  • Assay Principle

    • Proteins from various samples (cell lysates, serum, saliva, etc.) are captured on membranes with antibodies.
    • Detected via a biotinylated cocktail and chemiluminescent reagents, creating a signal indicative of protein levels.

  • Technical Hints

    • Only for research; not diagnostic.
    • Use within expiration date; avoid contamination.
    • Follow precise handling and washing protocols to ensure assay integrity.

  • Storage Conditions

    • Store unopened kit at 2-8 °C; opened components have specified storage conditions.

  • Required Supplies

    • Pipettes, PBS, lysis buffer, various reagents and equipment for sample handling.

  • Sample Collection and Preparation

    • Guidelines for preparing various sample types (cell culture supernatants, serum, etc.) to maintain integrity.

  • Reagent Preparation

    • Step-by-step instructions for preparing buffers and reagents prior to assay.

  • Protocol Steps

    • Series of steps detailing the assay process, from blocking membranes to detection and signal generation.
    • Includes thorough wash steps crucial for reducing background and enhancing signal clarity.

  • Data Analysis

    • Guidelines for analyzing signals captured on X-ray film, including template setup and pixel density assessment.

  • Profiling Proteins

    • Ability to profile proteins across various types like cell culture supernates, body fluids, and tissue lysates with specific sample amounts outlined.

  • Appendix

    • Comprehensive listing of analytes, their corresponding coordinates on the array, and related gene identification numbers.