Enzymes are:
- Proteins
- catalysts, which speed up reactions by a lot and do this by lowering activation energy which is the energy needed for a reaction to occur
- specific
- reusable
- affected with environmental conditions
- and often end in -a s e like lactase
Enzymes have active sites, which are locations where they interact with reactants, Substrates, in a reaction
- these sites are Special in shape, like lock and key, which means you can't use just any.
- and they aren't used up in reactions
- they denature if environments are not in their optimal range which means best conditions and can include:
- temp
- Salinity
- pH
- these can change the active site shape which means no product
Homeostasis is maintaining a stable, internal environment, energy is expensive
Can assist in:
growth development
- move materials
- build new molecules
-responding to the environment
-temperature regulation
Energy forms are:
- light
electricity
-heat
- can also be found in the bonds that keep molecules together
-A T P stores and releases energy and is the currency of cells
-A T P is Adenosine triphosphate and is made up of:
- Adenine
- ribose
- 3 phosphate groups which are negative
• There are two main types of inhibitors:
Non-competitive Inhibitors and competitive inhibitors
- Competitive inhibitors compete For access to the active site and prevent enzymes to catalyze reactions efficiently
- These would not stop a reaction from occurring completely because it would require an inhibitor to be in EVERY active site but it can still slow down a reaction
- Non-competitive inhibitors bind to an enzyme on a site called the allosteric Site that then causes the shape of the active site to change
- With an active site that is a different shape, it can't speed up the reaction because the reactants aren't the right they are not permanent and are reversible
-allo means different, so it is a different site to change the active site
- Photosynthesis: The process by which plants, algae, and even some bacteria convert LIGHT energy to chemical energy (GLUCOSE).
- Another name for a plant, algae, and/or bacteria that can photosynthesize is Autotroph (can make its own food)
- Other organisms obtain their chemical energy by consuming other living things and are called heterotrophs
Chloroplasts:
Specialized structures that make photosynthesis possible
Stroma - fluid portion of chloroplasts
Thylakoids - sac-like membranes where photosynthesis takes place
a stack of thylakoids is called a granum
Contain pigments whose job is to absorb sunlight (example:
chlorophyll)
CHLOROPHYLL IS A PIGMENT
Pigments = light capturing structures
Found in the membranes of thylakoids
Most well-known photosynthesis pigment is chlorophyll
look at light absorption spectrum and determine what colors of light chlorophyll absorbs best
Chemical equation:
6CO2+6H2O → C6H12O6 + 6O2, carbon dioxide and water are converted into glucose and oxygen with help of light
Light-dependent reactions
Occurs in thylakoids
Requires light
Produces ATP and
NADPH
Light-independent reactions (Calvin
Cycle)
Occurs in stroma
Does not require water
Uses ATP and NADPH to do functions
Light dependent reactions follow these steps:
Light hits a photosystem and excites electrons
The electron follows the Electron Transport Chain (ETC) to the second photosystem
The other electron that was in the second photosystem follows another ETC to NADP+ creating NADPH
While that happens, hydrogens travel from the outside to the inside of the thylakoid membrane
They come back up through ATP Synthase, spinning it and creating ATP
To recycle, Water is broken apart for the electron, the hydrogen joins the others and oxygen becomes a byproduct
Light Dependent reactions (Calvin Cycle) works as follows:
Carbon Fixation
3 molecules of CO2 join to RuBP (5 carbon sugar) by rubisco
Reduction
The 6 carbon compound is split into two 3 carbon molecules because it was unstable at the beginning
They split and reform using energy from ATP and NADPH from the light dependent reactions
The six 3 carbon molecules are known as G3P
There is a net gain of three carbons, which form one half of a glucose molecule
Once the process is done TWICE, then one full glucose will be formed when they connect
Regeneration
Using ATP, (Making it ADP and a phosphate) The remaining 5 G3P molecules are rearranged to create RuBP
BROAD OVERVIEW OF THE ENTIRE PROCESS PHOTOSYNTHESIS:
The Light Reactions (Phase 1) capture the energy in sunlight and convert it to chemical energy in the form of ATP and NADPH through the use of photosystems, electron transport chains, and ATP
Synthase
The Calvin Cycle (Phase 2) uses the energy transformed by the light reactions along with carbon dioxide to produce glucose.
DEFINITION OF CELLULAR RESPIRATION
The controlled release of energy from food (commonly glucose) in the presence of Oxygen.
The chemical reaction of cellular respiration is the REVERSE of photosynthesis
Cellular Respiration and Photosynthesis are said to be COUPLED
What one produces the other needs
C6H12O6+602 —> 6CO2+6H2O+Sunlight (opposite of photosynthesis equation)
Happens in four steps
Glycolysis
Pyruvate Conversion
Krebs Cycle (Citric Acid Cycle)
Electron Transport Train/ATP Synthesis
MOST OF CELLULAR RESPIRATION HAPPENS IN MITOCHONDRIA
Outer Membrane
Inner membrane
Space between membranes is called intermembrane space
Matrix = Fluid of mitochondria
Cristae = folds of inner membrane
CELLULAR RESPIRATION
STEP 1:
Glycolysis
Glyco-= glucose
-lysis = to break
Happens in CYTOPLASM of cell
Glucose → 2 pyruvate molecules
Investment - spend 2 ATP
Payoff - produce 4 ATP
OVERALL = +2 ATP
Also creates electron carriers called NADH
PYRUVATE CONVERSION
STEP 2:
• This process occurs in the intermembrane space of
occurs when oxygen is available
2 pyruvate → 2 acetyl coA molecules
Molecules produced in this step - carbon dioxide, NADH, and acetyl coA
KREBS CYCLE/CITRIC ACID CYCLE
STEP 3:
Occurs in the matrix of mitochondria and only occurs when oxygen is
The final breakdown of what is left from what was glucose
• Products formed = carbon dioxide, NADH, FADH2 and 2 ATP
ELECTRON TRANSPORT CHAIN/ATP SYNTHESIS
The electron transport chain (ETC) is located in the inner membrane of the mitochondria and its main purpose is to produce energy in the form of ATP.
Step 1: Electron Donation
NADH and FADH₂, which are made during glycolysis and the Krebs cycle, donate their electrons to the ETC. When they do this, NADH becomes NAD⁺ and FADH₂ becomes FAD.
Step 2: Electron Flow
The donated electrons move through a series of protein complexes in the ETC. This movement releases energy, which is used to pump hydrogen ions (H⁺) from the mitochondrial matrix to the intermembrane space, creating a concentration gradient.
Step 3: ATP Production
The hydrogen ions then flow back into the matrix through a protein called ATP synthase. This flow provides the energy needed to convert ADP into ATP.
Step 4: Water Formation
At the end of the chain, electrons combine with oxygen (the final electron acceptor) and hydrogen ions to form water (H₂O).
Final Products
From the ETC, around 34 ATP molecules and water are produced. The carriers, NAD⁺ and FAD, are recycled to be used again in earlier steps of cellular respiration.
FERMENTATION
What if oxygen isn't available for cellular respiration?
Step 1 (Glycolysis) of Cellular
Respiration will always occur in the cytoplasm WITH or WITHOUT oxygen.
Pyruvate conversion, Krebs Cycle, and ETC & ATP Synthesis will not be able to occur.
A process called fermentation will occur to allow only a small amount of ATP to be produced
Lactic Acid Fermentation
Typically occurs in animals and some bacteria
This is the type of fermentation that humans do when cells have limited or no oxygen available
Example: muscle cells during intense exercise
This type of fermentation is also responsible for yogurt, cheese, and pickles
ALCOHOLIC FERMENTATION
Occurs in plants, yeasts, and some bacteria
2 Pyruvate + 2 NADH → 2 NAD* + 2 CO2 + 2 Ethanol
Wine, beer, bread