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Statistics and Analytical Chemistry

Course Overview

  • Course Instructors:
  • Analytical Chemistry: Rime Bahij, Lars Duelund, Michael Pedersen
  • Statistics: Mathias Porsmose Clausen
  • Laboratory Exercises: Lars Duelund, Mette Boesgaard
  • Project Supervisors: Rime Bahij, Lars Duelund, Mathias Porsmose Clausen

Teaching Plan

  • Weekly Schedule (Weeks 6-14):
  • Lectures:
    • 4 lectures in Analytical Chemistry
    • 4 lectures in Statistics
  • E-Classes: Approximately 2 per week
  • Attendance: 80% mandatory for FA507 students
  • Semester Project Breakdown:
  • Week 7: IGT students start with project; group creation (email group members to instructor)
  • Week 8: Upload project description
  • Week 10: Upload literature review on chosen topic
  • Week 12: Upload experimental plan with list of chemicals
  • Weeks 13 & 14: Project presentations
  • Report Deadlines:
    • IGT: May 30
    • FKF: April 4, 2025
  • Lab Classes for FKF Students: 3x4 hours during weeks 10-12, attendance is required to pass the exam

Exam Structure

  • IGT Students:
  • Group exam + report
  • Exam times to be announced
  • FKF Students:
  • Written exam (4 hours)
  • Two parts: Multiple Choice (30%), Written Part (70%)

Recommended Textbooks

  • Principles of Instrumental Analysis, 7th Edition by Skoog, Holler & Crouch
  • Practical Statistics for the Analytical Scientist by Stephen LR Ellison, Vicki J. Barwick, and Trevor Duguid Farrant.

Key Concepts in Analytical Chemistry

  • Definition: The scientific discipline that develops and applies methods, instruments, and strategies to understand the composition and nature of matter.
  • Focus on the identification and quantification of substances.
  • Role in various fields:
  • Chemistry, Biochemistry, Environmental Science, Pharmacy, Forensics, etc.

Categories of Analytical Chemistry

  • Quantitative Analysis: Determines the amount of analyte present.
  • Qualitative Analysis: Identifies the components of a sample.
  • Types of Analysis:
  • Gravimetric Methods: Measure mass.
  • Volumetric Methods: Measure liquid volume containing reagents.
  • Qualitative and Quantitative Methods: Differentiating what and how much is present.

Chromatography Details

  • Chromatography Overview:
  • Techniques: Gas Chromatography (GC), High-Performance Liquid Chromatography (HPLC), and others.
  • Separation based on differing affinities for stationary and mobile phases.
  • Components:
  1. Injector: Introduces sample.
  2. Column: Contains stationary phase.
  3. Detector: Observes and quantifies separated components.

Chromatographic Terms and Factors

  • Key Terms:
  • Retention Time: Time it takes for a compound to pass through the column.
  • Retention Factor (k): Ratio of concentrations in stationary to mobile phase.
  • Selectivity Factor (α): Measures how well two analytes are separated.
  • Peak Symmetry: Describes distribution profiles of chromatographic peaks, indicating resolution quality.

Performance Metrics in Chromatography

  • Column Efficiency: Assessed by theoretical plates (N) and resolution (Rs).
  • Resolution Equation: Ties together the number of plates, retention, and separation factors, indicating how distinct peaks are in output.

Practical Applications of Analytical Chemistry

  • Fields of Application:
  • Clinical, Forensics, Environmental, Industrial, Research laboratories, among others.
  • Important Analytical Techniques:
  • Spectroscopy, Mass Spectrometry, Electroanalytical methods, etc.

Sample Preparation Steps

  • Key Steps:
  1. Formulating the question.
  2. Selecting the analytical procedure.
  3. Sampling representative material.
  4. Preparing the sample (storage, grinding, dissolving, concentration).

Conclusion

  • Importance of Analytical Chemistry: Key role in advancing science across multiple disciplines, contributing to knowledge and technology in chemistry, biology, medicine, and more.

Exercises and Further Reading

  • Suggested Readings: Chapter 26 from recommended textbooks.
  • Exercises: Practice problems from textbook to solidify understanding of concepts in chromatography and analytical methods.