Statistics and Analytical Chemistry
Course Overview
- Course Instructors:
- Analytical Chemistry: Rime Bahij, Lars Duelund, Michael Pedersen
- Statistics: Mathias Porsmose Clausen
- Laboratory Exercises: Lars Duelund, Mette Boesgaard
- Project Supervisors: Rime Bahij, Lars Duelund, Mathias Porsmose Clausen
Teaching Plan
- Weekly Schedule (Weeks 6-14):
- Lectures:
- 4 lectures in Analytical Chemistry
- 4 lectures in Statistics
- E-Classes: Approximately 2 per week
- Attendance: 80% mandatory for FA507 students
- Semester Project Breakdown:
- Week 7: IGT students start with project; group creation (email group members to instructor)
- Week 8: Upload project description
- Week 10: Upload literature review on chosen topic
- Week 12: Upload experimental plan with list of chemicals
- Weeks 13 & 14: Project presentations
- Report Deadlines:
- IGT: May 30
- FKF: April 4, 2025
- Lab Classes for FKF Students: 3x4 hours during weeks 10-12, attendance is required to pass the exam
Exam Structure
- IGT Students:
- Group exam + report
- Exam times to be announced
- FKF Students:
- Written exam (4 hours)
- Two parts: Multiple Choice (30%), Written Part (70%)
Recommended Textbooks
- Principles of Instrumental Analysis, 7th Edition by Skoog, Holler & Crouch
- Practical Statistics for the Analytical Scientist by Stephen LR Ellison, Vicki J. Barwick, and Trevor Duguid Farrant.
Key Concepts in Analytical Chemistry
- Definition: The scientific discipline that develops and applies methods, instruments, and strategies to understand the composition and nature of matter.
- Focus on the identification and quantification of substances.
- Role in various fields:
- Chemistry, Biochemistry, Environmental Science, Pharmacy, Forensics, etc.
Categories of Analytical Chemistry
- Quantitative Analysis: Determines the amount of analyte present.
- Qualitative Analysis: Identifies the components of a sample.
- Types of Analysis:
- Gravimetric Methods: Measure mass.
- Volumetric Methods: Measure liquid volume containing reagents.
- Qualitative and Quantitative Methods: Differentiating what and how much is present.
Chromatography Details
- Chromatography Overview:
- Techniques: Gas Chromatography (GC), High-Performance Liquid Chromatography (HPLC), and others.
- Separation based on differing affinities for stationary and mobile phases.
- Components:
- Injector: Introduces sample.
- Column: Contains stationary phase.
- Detector: Observes and quantifies separated components.
Chromatographic Terms and Factors
- Key Terms:
- Retention Time: Time it takes for a compound to pass through the column.
- Retention Factor (k): Ratio of concentrations in stationary to mobile phase.
- Selectivity Factor (α): Measures how well two analytes are separated.
- Peak Symmetry: Describes distribution profiles of chromatographic peaks, indicating resolution quality.
- Column Efficiency: Assessed by theoretical plates (N) and resolution (Rs).
- Resolution Equation: Ties together the number of plates, retention, and separation factors, indicating how distinct peaks are in output.
Practical Applications of Analytical Chemistry
- Fields of Application:
- Clinical, Forensics, Environmental, Industrial, Research laboratories, among others.
- Important Analytical Techniques:
- Spectroscopy, Mass Spectrometry, Electroanalytical methods, etc.
Sample Preparation Steps
- Formulating the question.
- Selecting the analytical procedure.
- Sampling representative material.
- Preparing the sample (storage, grinding, dissolving, concentration).
Conclusion
- Importance of Analytical Chemistry: Key role in advancing science across multiple disciplines, contributing to knowledge and technology in chemistry, biology, medicine, and more.
Exercises and Further Reading
- Suggested Readings: Chapter 26 from recommended textbooks.
- Exercises: Practice problems from textbook to solidify understanding of concepts in chromatography and analytical methods.