Paper chromatography is a separation technique used to separate substances in a mixture.
It is a physical process, meaning it does not involve chemical reactions or the creation of new substances.
Other separation techniques previously discussed include:
Filtration
Crystallization
Simple distillation
Fractional distillation
Chromatography is another method utilized by scientists for the separation of mixtures.
Paper chromatography separates substances based on their different solubilities.
Materials Needed:
Special chromatography paper
Colored pens (to demonstrate the separation of colors)
Solvent (liquid that dissolves substances)
Procedure:
Draw a pencil line near the bottom of the chromatography paper.
Place dots of different colors on the pencil line.
Immerse the bottom of the paper in a solvent.
Key Terminology:
Stationary Phase: The paper, which does not move.
Mobile Phase: The solvent, which moves and carries substances with it.
After some time:
A pure color (e.g., red) will form a single spot on the paper, indicating it is a single pure substance.
A mixed color (e.g., color B) will separate into two or more spots, indicating it contains multiple substances.
The same experiment can yield varying results with different solvents:
A pure compound will always produce a single spot regardless of the solvent used.
Mixtures may separate into different spots depending on the solvent.
Different substances have different solubilities.
A more soluble substance is more attracted to the mobile phase (solvent) and will travel further up the paper.
It is crucial to draw the starting line in pencil rather than pen:
Pen ink may dissolve in the solvent and contaminate the results.
The concept of paper chromatography will be revisited in later topics, particularly in chemical analysis.
It will be used to identify compounds by calculating the RF value (retention factor).
A revision workbook related to chromatography concepts and questions is available for further study.