Cellular Respiration Overview
Cellular Energy
- Energy in Cells
- Cells constantly expend energy in the form of ATP hydrolysis.
- ATP (Adenosine Triphosphate) hydrolysis converts ATP into ADP (Adenosine Diphosphate) and a phosphate group (P).
- Reaction: ATP → ADP + P
- After hydrolysis, ATP is resynthesized from ADP and P.
- Reaction: ADP + P → ATP (recycling energy).
- Cells utilize stored energy from monosaccharides, fatty acids, and amino acids to resynthesize ATP.
Fuels for ATP Synthesis
Key Fuels:
- Proteins: Derived from amino acids.
- Carbohydrates: Mainly glucose and other sugars, stored in the form of glycogen.
- Fats: Including glycerol and fatty acids.
Stages of Metabolism:
- Stage 1: Digestion (in GI tract)
- Stage 2: Anabolism and formation of catabolic intermediates within tissue cells.
- Stage 3: Oxidative breakdown in mitochondria, leading to oxidative phosphorylation in the electron transport chain.
Cellular Respiration
- Main Process: Most cells use glucose with oxygen (O2) to synthesize ATP.
- Overall reaction: C6H12O6 + 6O2 + 38 ADP + 38 P → 6H2O + 6CO2 + 38 ATP.
- Notable: The number of atoms in reactants equals those in products.
- Energy Utilization: ATP produced is used for:
- Muscle contraction,
- Ciliary beating,
- Active transport,
- Synthesis reactions.
Phases of Cellular Respiration
Glycolysis:
- Occurs in the cytoplasm.
- Breaks down glucose into 2 molecules of pyruvic acid through 10 steps.
- Produces a net of 2 ATP and 2 NADH.
Krebs Cycle (Citric Acid Cycle):
- Takes place in mitochondrial matrix.
- Processes Acetyl CoA derived from pyruvic acid.
- Produces NADH, FADH2, CO2, and ATP through a cyclic series of reactions.
Electron Transport Chain (ETC):
- Located across the inner mitochondrial membrane.
- Uses electrons from NADH and FADH2 to establish a proton gradient, powering ATP synthesis via oxidative phosphorylation.
Glycolysis Detail
Overview:
- Glycolysis translates to "sugar breaking".
- Begins with 1 glucose (6 carbons) and ends with 2 pyruvic acid (3 carbons each).
Steps:
- Energy investment of 2 ATP in the initial steps.
- Splitting of a 6-carbon sugar into two 3-carbon intermediates.
- NAD+ is reduced to NADH as 3-carbon intermediates lose H atoms.
- Total gained ATP: 2 (net), 4 produced but 2 used.
Final Products:
- 2 ATP (net), 2 pyruvate, 2 NADH per glucose.
Pyruvic Acid and Respiration Types
Aerobic Respiration:
- If O2 is present, pyruvic acid converts to Acetyl CoA, leading to Krebs cycle.
- Produces an additional 36 ATP.
Anaerobic Fermentation:
- Occurs in absence of O2, converting pyruvic acid to lactic acid.
- Result: no additional ATP, causes muscle fatigue and soreness.
Krebs Cycle (Citric Acid Cycle) Detail
- Process:
- Involves the conversion of Acetyl CoA (2 C) into citric acid (6 C) through various enzymatic steps, decarboxylating and oxidizing.
- Key outputs: 3 NADH, 1 FADH2, 2 CO2, and 1 ATP per cycle.
- Since each glucose yields 2 Acetyl CoA, total from 2 cycles: 6 NADH, 2 FADH2, 4 CO2, 2 ATP.
Electron Transport Chain (ETC)
- Function:
- Comprised of integral proteins creating an H+ gradient in the mitochondria.
- NADH and FADH2 donate electrons; this energy is used to pump H+ ions across the membrane, enhancing the H+ gradient.
- ATP Synthesis:
- H+ flows back through the ATP synthase, facilitating ATP synthesis (oxidative phosphorylation).
- NADH yields ~3 ATP, FADH2 yields ~2 ATP.
Conclusion
- Overall ATP yield from cellular respiration: Approximately 38 ATP (2 from glycolysis, 2 from Krebs cycle, 34 from ETC via oxidative phosphorylation).
- Final consumption of electrons leads to the formation of water as O2 accepts electrons.