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Microbiology Exam Notes

Microorganisms & Cellular Biology
  • Candida albicans: Most frequently isolated yeast.

  • Pinworm: Common parasite in children worldwide.

  • Spirilla: Elongated, spiral-shaped, rigid bacterial cells.

  • Prokaryotes: Organisms defined as "before nucleus."

  • MRSA: Responsible for difficult-to-treat infections in humans.

  • Virus: Causes infections by entering and multiplying inside human host cells.

  • Nucleus: Contains a cell's hereditary information and controls its growth/reproduction.

  • Tetrad: Cells remaining in groups of four, dividing in two planes.

  • Obligate aerobes: Require O_2 to grow.

Laboratory Media & Equipment
  • EMB (Eosin Methylene Blue agar): Contains dyes toxic to Gram-positive bacteria.

  • HEPA Filter: Used to filter air in biological safety cabinets.

  • Agar: Seaweed extract; liquid when heated, solid when cooled.

  • Thayer[-Martin]: Used to recover Neisseria gonorrhoeae.

  • SBA (Sheep Blood Agar): Most bacteria grow; provides a total colony count.

  • Charcoal/Charcoal resin: Neutralizes bacterial toxins/inhibitory substances and absorbs antibiotics.

  • Slant: Solid media culture in a tube for storage, transport, or biochemical testing.

  • Autoclave: Uses steam under pressure for sterilization.

Infection Control & Diagnostics
  • Mycology: The study of fungi.

  • Nosocomial infection: Infection acquired during treatment in a health care facility.

  • Species Taxonomy: Provides a mechanism for identification and grouping of living things.

  • FIT (Fecal Immunochemical Test): An alternative test to FOBT.

  • Vector: Refers to infections caused by insects and animals.

  • Disinfection: Process using agents like bleach, phenol, alcohols, or aldehydes.

  • Radiation: A secondary method of disinfection.

  • Moist heat: Destroys organisms by denaturing proteins.

  • Sensitive: Describes an organism whose growth is inhibited by an antibiotic.

  • Biohazard: A biological agent or material potentially hazardous to life forms.

  • Uncontaminated: Refers to keeping a sample free from unwanted substances.

Key Biological & Medical Terms
  • Sputum: Contains ciliated columnar epithelial cells; used as a sample, or alternatives are employed when a patient cannot produce a sample.

  • Aerosol: Liquid or particulate suspended in a gaseous medium.

  • Organism: Something that lives on or in a host organism.

  • Culture Monitoring: Most cultures are monitored for 5 days.