MLSP microbio

Class of biosafety cabinet that allows room air to pass into the cabinet, consists of negative pressure, and operated in open front

Class I

Class of biosafety cabinet where air flows in "sheets”, which serves as a barrier to particle from outside cabinet

Class II

Other name for class II biosafety cabinets

Vertical laminar flow BSCs

Class of biosafety cabinet that is self-contained, and 70% of the air is recirculated

Class IIA

Class of biosafety cabinets selected fro radioisotopes, toxic chemicals, or carcinogenic samples

Class IIB

An organism that is said to be microscopic

Microbes

Very simple microbes consisting of nucleic acid, a few proteins, and lipid envelopes in some. They are completely dependent on the cells they infect for survival

Viruses

Microbes with both DNA and RNA, metabolic machinery for self-replicaiton, and a complex cell wall structure

Bacteria

Microbes subdivided into single-celled organisms(yeast_ or multicelled organisms(mold), with a few medically important members existing in both forms

Fungi

Microbes subdivided into single-celled organisms(protozoa) or multi-celled organisms(worms and bugs)

Parasites

Fungi that can exist as both single celled and multicelled organisms

Dimorphic fungi

T/F Bacteria are eukaryotic

F

Small circular molecules of extrachromosomal circular DNA

Plasmids

Primary way of reproduction of bacteria

Binary fission

Diameter of bacteria

0.2-2micrometers

length of bacteria

1 to 6 micrometers

Principal stain used for microscopic examination of bacteria that divides them into two large groups

Gram stain

The one who devised the gram stain in the 19th century

Hans Christian Gram

Bacteria that take up the basic dye and are crystal violet in color

Gram positive

Bacteria that allow crystal violet dye to wash out easily with decolorizer alcohol or acetone

Gram negative

Other term for cell wall

peptidoglycan

Cell wall of gram positive bacteria

Thick

Are lypopolysaccharides present in gram positive

Absent

Are lypopolysaccharides present in gram negative

Present

Color of the staining on gram positive

Purple

Color of the staining on gram negative

red or pink

peptidoglycan of gram negative

Thin

Bacteria with spherical shaped cells

Cocci

Bacteria with rod shaped cells

Bacilli

Bacteria with spiral shaped cells

Spirilla

Bacteria with comma shaped cells

Vibrios

Process by which bacteria catabolize carbohydrates to produce energy

Fermentation

Also known as oxidation, it is the process of bacterial energy generation rather than fermentation

Respiration

The standards followed in the collection and handling of specimens in microbiology

Universal precautions

Procedures that reduces contamination of microbes

Asceptic technique

How many cultures should be done on blood during a 24hr period

2 to 3

How much blood is needed for culture on adults

20-30ml

How much blood is needed on infants for a culture

1-5ml

Media that can grow microorganisms

Culture media

Media that supports the growth of a wide range of microorganisms and are considered non-selective because, the growth of most organisms are supported

Nutritive media

What type of media are blood agar plate and chocolate agar

Nutritive media

Media where microorganisms can be distinguished on the basis fo certain growth characteristics evident on the medium

Differential media

Bacteria that are hard to grow

Fastidious bacteria

Composition of blood agar plate

5% defibrinated sheep's blood

agar for fastidious bacteria

chocolate agar

Media that supports the growth of one group of organisms, but not another, by adding antimicrobials, dyes, or alcohol to a paraticular medium

Selective media

What type of media are MacConkey Agar

selective

What type of media are columbia agar with colistin and nalidixic acid

Selective

Used for the detection of small numbers of organisms present

Backup broth

ANother term for backup broth

Supplemental or enrichment broth

Agar that inhibits gram positive bacteria

MacConkey Agar

Agar that inhibits gram negative cells

Columbia agar with colistin and nalidixic acid

Media used when specific organisms are suspected

Specialized media

Agar for staphylococcus species

Mannitol salt agar

Agar for salmonella species

Bismuth sulfite agar

Agar for vibrio species

Thiosulfate-citrate-bile-salts-sucrose(TCBS) agar

Agar for campylobacter species

campylobacter agar

temperature for normal incubation of bacterial cultures

35-37 C

Materials appropriate for anaerobic incubation cultures

Anaerobic jars, bags, and chamber

Temperature for stool cultures

42-45 C

What is detected in stool cultures

Campylobacter jejuni