Entomology - Exam 3

introduction

  • 360,000 phytophagous (herbivorous) insects
      * ^^26% of species^^

    degree of specialization
    * monophagy: eat few things, very picky
    * oligophagous: narrow range and food
    * polyphagous: open feeders (still picky)

  • insects originated 400mya
      * fed on nonvascular plants
      * first fed on pollen and pollen cones
        * then reproductive parts of plant
        * eventually would eat leaves and other parts

History of Interactions

  • ^^angiosperms appear in Cretaceous, promoted radiation in plants/insects^^
      * insects initially herbivorous to be antagonistic with plants
      * ^^evolved to be mutualistic to help plants reproduce^^
        * ^^bees, butterflies, beetles, flies^^
  • insects promoted speciation in plants through pollinator-mediated reproductive isolation
  • ^^phytophagy feeding strategies^^
      * leaf chewing
      * sap sucking
        * eat cell sap and phloem
      * seed predation
      * gall induction
      * mining (tunnels) and boring (holes)

^^theory of herbivory^^

  • assumptions
      * ==damage by insects is a major selective force==
      * ==nutritional quality has a strong influence on abundance and damage caused==
  • varied herbivore rates
      * nutritional quality
        * some insects prefer stressed plants
          * boring and sucking insects
        * some prefer fast growing plants
          * gall inducers and chewers

co-evolution

  • reciprocal interactions (adaptions) between two different clades that are selective forces on each other
      * they keep responding back and forth to each other
      * Ehrlich & Raven - 1964

primary metabolites

  • compounds that are needed for cells to be alive
  • examples
      * enzymes
      * hormones
      * carbs
      * lipids
      * proteins
      * phosphorous

plant defense

  • coevolutionary process from species interactions
  • produce compounds not needed to survive
      * secondary metabolites / allelochemicals
      * chemicals involved in interspecific communication
      * examples
        * @@glucosinolates: mustard oils@@ - Brassicaceae
          * irritates animal skin
          * most potent antibiotic from plants
          * Phyllotreta cruciferae - Flea Beetle
        * @@alkaloids: nitrogenous-based comps@@ - Solanaceae
          * nicotine - Nicotina
          * solanine - Solanum tuberosum
          * Manduca sexta - Tomato hornworm moth is specialist
        * @@cardiac glycosides@@: Asclepidaceae & Apocynaceae
          * milky latex of plants
          * Euchaetias & Cycnia are specialist genera
  • some secondary compounds are highly toxic to insects
      * nicotine
      * sinigrin - mustard glucoside
  • @@other secondary metabolites influence fecundity and population growth of herbivore populations@@
      * tannins & phenolics
        * precipitate proteins
          * turns to a solid and makes protein unusable
        * Operophtera brumata - Winter moth larvae
      * endopeptidase inhibitors
        * reduce digestion of proteins
        * cant breakdown
      * juvabione
        * juvenile hormone mimic, keeps insect as nymph
        * reduces population size
        * Pyrrhocoris apterus
      * ecdysone-like compounds
        * stimulates shedding of exoskeleton (molting)
        * creates small adults with reduced fecundity
        * Podocarpus & Polypodium
  • allocation of secondary compounds
      * tend to be concentrated in structures at risk of being eaten (reproductive structures)
      * examples
        * THC - seeds of Cannibis
        * pyrethrum - flowerheads from chrysanthemums
      * requires trade off in biomass allocation from other structures
        * Acyanogenic & cyanogenic morphs of Lotus corniculatus
          * response to being eaten by slugs
          * cyanogenic morph makes less seeds and has slower growth
  • @@responses of insects to plant defense@@
      * @@evolved enzymes to metabolize toxic compounds@@
        * microsomal mixed-function oxidases in some Lepidoptera
          * break down pyrethrum w/ oxidases
      * counterattack by chrysanthemums
        * produces sesamin which inhibits mixed function oxidases
      * @@ability to metabolically recognize toxins@@
        * Dioclea megacarpa makes nonprotein amino acid
          * Canavanine
          * highly toxic to most herbivores bc its so similar to arginine
        * Bruchid beetle - Caryedes brasiliensis
          * can distinguish between two amino acids w. tRNA
          * has lack of parasitoids as consequence
      * @@temporal avoidance of toxins@@
        * winter moth feeds on oaks by hatching early in spring
          * avoids concentrated tannins
          * im
      * @@sequestering of toxin@@
        * specialists of plants unaffected by toxin, hold it in fatty tissue

    * monarchs feed on milkweed, parasitized by tachinid fly

  • Insect Response
      * Aposematic coloration
        * bright coloration advertises prey on purpose
        * insect is toxic, trains predator to leave it alone
          * palatability spectrum
          * Lygaeidae - Milkweed Bugs
  • @@Plant Counter Attack@@
      * alternate defense methods
        * produce gum that pushes off egg
        * exploding fruits to disperse seed
        * indehiscent fruits for ovipositors that lay on exposed seeds
        * seed surface swells to detach eggs
        * seeds too thin for larvae to mature in
        * seeds to small for larvae to mature in
  • evolution of plant defense compounds
      * plant apparency hypothesis
        * Feeny - 1971
        * described extreme endpoints
        * @@apparent plants@@
          * late successional habitat plants
          * %%have quantitative defenses that are metabolically expensive%%
            * %%have dosage effect%%
          * regulate population compounds
        * @@unapparent plants@@
          * early successional plants
            * wont be around long, short lived
          * %%have qualitative defenses that are metabolically inexpensive%%
            * %%have large toxic effect%%
            * %%rapid irritant or lethal effect%%
          * secondary compounds are highly toxic and function to kill
          * R-selected plants select for R-type insects

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pollination

why is it important

  • part of sexual reproduction

function of flowers

  • to attract pollinators
      * color petals
      * scent
      * rewards
        * nectar, pollen, & oviposition sites
  • floral organs
      * sepals: outer whorl (collectively calyx)
      * petals: second whorl (collectively corolla)
      * stamens: third whorl
      * ovary: inner whorl (collectively carpel)

Coevolution

  • coevolution: reciprocal adaptations between interacting species that increases their codependence
      * plants and insects evolve around each other to keep getting pollinated
  • Darwin’s Classic Example
      * orchids adapt to benefit pollinators with a long proboscis
        * Angraecum sesquipedale
        * nectar spur up to 30cm long, Darwin predicted something must match

Pollination Syndromes

  • %%Beetles: Cantharophily%%
      * flowers open at night
      * white color, strong fruit smell
      * petals are food reward, no nectar
      * flowers form a chamber to house beetles
      * Magnolias
  • %%Flies: Myophily%%
      * flowers open during day and near ground
        * looking for place to lay eggs
      * rotting, decaying flesh smell
      * green to maroon color
      * Symplocarpus foetida (Skunk Cabbage)
  • %%Butterflies: Psychophily%%
      * flowers open during day
      * bright colors
      * corolla throat is narrow
        * match proboscis
      * corolla spur to restrict visitors
      * Phlox drummondii (Woodland Phlox)
  • %%Moths: Phalaenophily%%
      * flowers open in twilight hours
        * Crepuscular
      * white in color
      * lots of nectar
        * moths hover so they need more carbohydrates
      * long corolla tube matches proboscis
      * exterted anthers and stigmas
        * pollen is sticky
      * Datura (Jimson weed)
      * Oenothera missouriensis (Missouri evening primrose)
  • %%Bees: Melittophily%%
      * Enclose anthers and stigmas to only let in strong bees
        * must push open to get to reproductive organs and nectar
      * bright colored flowers w/ nectar guides
        * spots or lines
      * flowers reflect UV light and look like target
      * not much nectar, sweet smell

Decline in Pollinators

  • Honeybees
      * 1/3 of every bite of food is dependent on pollinators
      * Colony Collapse Disorder
        * most likely from widespread use of pesticides and fungicides
          * fungicides mainly kill larvae
        * spread of viral pathogens
        * parasitic mites in hives
        * bees get confused while out in the field and never return to the hive

Bee Adaptations to Plants

  • colorvisionininsectsisdifferentcolor vision in insects is different
      * yellow green
      * blue
      * UV as a distinct color
      * Iris pseudocornus
  • Electrostatic charge
      * beesgetpositivechargewhenflyingaroundbees get positive charge when flying around
        * flowershavenegativechargeflowers have negative charge
      * opposite charges makes the pollen jump from flower to bee
      * bees are able to sense charge difference
        * chargedoneshavebetteryieldcharged ones have better yield
  • thickhairthick hair
      * lets bumblebees forage when its too cool for honeybees
      * makes bumblebees important pollinators in colder climates
  • branchedhairsbranched hairs
      * allows more pollen to stick to bees
      * NOT IN WASPS just bees
  • stouthairsstout hairs
      * hairs on tarsus used to comb pollen off body onto back legs
      * strong spirally ridged hairs
  • oilcollectinghairsoil collecting hairs
      * scoop shaped hairs on tarsal segments to pick up oils
      * rasp their legs across flowers]
      * Centris bees (not in Missouri)
  • Corbicula(PollenBaskets)Corbicula (Pollen Baskets)
      * concavity on tibia that carries pollen
      * used for feeding to larvae (mixed with spit and nectar)
      * only in Apis and Bombus
  • ScopaScopa
      * similar to pollen baskets
      * dense mass of branched hairs pollen is pressed into on body
  • pollenpackerpollen packer
      * articulation between tibia and tarsus to compress pollen before moving to basket
  • pectenpecten
      * row of stiff hairs off tibia articulation of worker bees, similar to pollen packer
  • pollenbrushorcombpollen brush or comb
      * row of hairs use to get pollen off body

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insect sociality

  • ==solitary insects==
      * do not live in pairs or more
      * usually have one offspring to the one mother
  • ==subsociality in insects==
      * ==aggregations==
        * nonreproductive, gregarious interactions
        * cooperative foraging: feed together with silk or pheromones
        * sleep aggregations: Ammophila wasps
        * hibernation:
        * migratory aggregations: monarchs
        * share nest entrance: solitary bees
        * aposematic phytophagus insects aggregate as a color warning
          * milkweed bugs
      * ==parental care==
        * rare in insects
        * ==some tend young (egg and early instars)==
          * Blattodea
          * Orthoptera
          * Hemiptera
          * Dermoptera
        * care w/o nest
          * some Passalid beetles have all life stages living together
            * adults chew wood for larvae to feed
          * treehoppers delegate parental care to ant by producing honeydew for ants to eat
        * care w/ nest
          * dung and carrion (Nicrophorus) beetles
          * aculeate hymenoptera - muddobbers
            * linear or radial multicelled
            * nests made from soil, mud, sand
            * tunnels seal with leaves, resin, mud, oils
            * Spider wasp - Pompilidae
              * nest with larvae at various ages that feed on spider
        * communal nesting
          * daughters nest in natal nest
  • ==eusocial insects==
      * ==3 defining features==
        * overlapping generations in same colony
        * reproductive division of labor with caste system
        * cooperative brood care
      * common features
        * make nest or containment structure
        * exhibit trophallaxis
          * exchange of substances
          * basis for maintaining social roles in colonies
      * ==only found in Blattodea (Termitoidea) and Hymenoptera==
        * Termitoidea
          * evolved sociality once (2000 species)
          * ==ancestral groups need symbionts to digest cellulose==
            * makes an enzyme that can break down cellulose
            * ==after each molt they need to be re-infected with symbionts through anal-feeding (anal trophallaxis)==
          * derived groups have evolved to not need symbionts
          * differ from Hymentopera:
            * undergo complete metamophosis
            * nymphs are workers
            * colony is bisexual
            * all individuals are diploid
          * share ancestor with cockroaches
          * most are sterile and never see the outside environmet
          * ==ancestral termites:==
            * reproductive adults
              * some become alates (winged) and leave and make new colonies
            * worker nymphs
              * males: majors, large
              * females: minors-small, majors-soldiers
          * ==derived termites: nasutitermes==
            * females: workers
            * males: soldiers that lack mandibles (nasutes)
            * both can squirt toxic liquid onto other things to blind them
          * reproduction
            * new colonies
              * formed from reproductive king and queen
              * winged reproductives leave after environmental cue
              * after dispersal flight they lose wings, make a nest, form copularium
              * young fed by trophallaxis
            * ==caste determination for reproductives==
              * reproductives
                * juveniles hormone
                  * hi juvenile hormone: given to colony by fecal trophallaxis, keeps them from becoming reproductives
            * nests
              * random shapes in soil, elaborate tunnels to regulate temp and humidity
        * %%Hymenoptera%%
          * evolved sociality 11 times (11000 species)
          * %%characteristics of hymenopteran societies%%:
            * colonies mainly females, males only for mating
            * castes are only reproductives and workers
            * caste determined by queen dominance, nutrition, or temp
            * colonies from single female, multiple females, or swarming
          * %%social wasps%%
            * ancestral wasps (paper wasps: Polistes)
              * small colony
              * several queens (polygynous)
              * %%dominant queen by aggression from high juvenile hormone%%
              * workers are sterile
              * %%nest is open, hangs by pedicel, and its smeared with ant resistant goop%%
            * derived (yellow jackets: Vespula)
              * large colony
              * %%one large queen (monogynous)%%
              * nest is enclosed w/ multiple layers, entrance guarded by workers
          * %%ants%%
            * ancestral species (9000 spp)
              * small colonies
              * little morphological difference in queen and workers
              * eggs laid in open nests
              * larvae produce naked pupae
            * derived species
              * large colonies, up to 20mil
                * small workers, large workers have long mandibles
              * larvae fed saliva from queen
              * larvae from silk cacoon
            * queens can live up to 20 years, workers 5-6 years
            * %%widest range of diet in all social insects%%
              * carnivores, scavengers, honeydew collectors
              * Atta grow fungus in gardens
              * adults feed larvae w/ fluids from integument
            * nests are not elaborate
              * gravel mounds in harvester ants
              * leafcutter ants in hollow acacia trees
          * %%social bees%%
            * evolved 8 independent times (1000 species)
            * ancestral species
              * little morph difference between queens and workers
              * casts kept by queen dominance
              * herbivores - eat pollen and nectar
              * brood cells used to store food
            * primitive eusocial tropical bees
              * stingless bees - Trigona & Melipona
              * 200 species
              * colonies formed by swarming
              * domesticated for honey production
            * %%primitively eusocial bees%%
              * bumblebees (Bombus) 200 species, temperate and tropical spp
              * overwintering queens make new colony in rodent burrows
              * larvae spin cocoon
              * workers- adults are all female and small, forage and tend larvae
              * males (drones) only produced in fall
            * %%advanced eusocial bees%%
              * honeybees (Apis)
              * 6-9 species
              * nest openly in trees/caves
              * nest guarded, lined with propolis
              * %%caste system kept by queen mandibular pheromone%%
              * new colonies
                * formed by swarming
                * new queens are fed royal jelly made by nurse bees
              * %%queens%%
                * larvae fed royal jelly
                  * high amounts of hexose sugars
                  * high juvenile hormone
                  * high amounts of mandibular secretion from workers
              * workers
                * larvae fed worker jelly
                  * low in hexose sugar and juvenile hormone
                * beebread: pollen and honey
              * %%honeybee biology%%
                * communication
                  * use polarized light to orient themselves off the sun
                  * %%communicate direction and distance using circle or figure eight dances%%
                * foraging
                  * pollen collected in pollen baskets (corbiculae) and honey crop
                * nest regulation
                  * keep nest at 30 degrees
                  * workers will fan nest w wings if it’s too hot
          * evolution of socialization in Hymenoptera
            * WD Hamilton (1964) - kin selection on the haplodiploidy form of reproduction
            * kin selection is altruistic traits/behaviors that favors another individual
              * daughters held raise sisters rather than their offspring
      * socialization
        * ==1/3 biomass in Amazon is termites and ants==
        * allows division of labor and more efficiency
        * ==inquilines - opportunistic organisms associated w colonies==
          * beetles, mites, and roaches might help defend
          * some are scavengers, predators, and parasites
      * ==evolutionary trends==
        * increased division of labor by castes, behavior, and morph
        * increased population size
        * daily provisioning
        * production of nest, open to enclosed
        * complex trophallaxis interactions w/ proctodeal and salivary substances
        * colonies have identity odor, restricts activity to own colony

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^^insect defense^^

  • insectivores: organisms that eat insects, usually specialized to eat them
  • insects have evolved wide range of defense mechs to avoid predation
  • defense strategies
      * ^^hiding - crypsis^^
        * ^^camouflage^^
          * resembles background of inedible object
          * uniform/patterned coloration: match background
            * Pentatomidae (shield bugs)
          * bizarre shape: disrupt body outline
            * Membracidae
          * countershading: light above, dark below on body
            * cicadas
        * ^^masquerade or mimesis^^
          * looks like object in nature, not another organism
          * often look like
            * twigs, leaves, bird poop, flowers, etc
      * ^^secondary lines of defense^^
        * fake dead: thanatosis or reflex mobility
          * weevils
        * startle displays
          * flash of color or spots to startle predator
          * bright flashes of color, eye spots
          * moths, grasshoppers
      * ^^mechanical^^
        * horns & spines
          * dung beetles
        * flattened dorsal/ventrally (biting lice) or laterally (fleas)
        * retreats or shelters (caddisflies)
        * artificial shields (Chyrsomelid & reduvidae)
        * waxes & powders in predators mouth
        * detaching body structures
      * ^^chemical^^
        * secrete allomones
          * positive effect on producer, negative on receiver
        * typically aposematically colored as a warning
          * brightly colored
        * chemical defense compounds derived from plants
        * ^^classifications^^
          * @@class 1:@@
            * irritate, poison, hurt
            * more specific to vertebrates
            * cyanides and alkaloids
            * aposematic
          * @@class 2:@@
            * anti-feedant, odor or taste inhibitors
            * volatile compounds
            * aromatic ketones, aldehydes, acids, and terpenes
          * ^^defense organs^^
            * evaginating storage gland to compound
            * urticating (stinging) hairs
            * venom stingers
            * osmeteria (pouch w/ toxins)
      * acoustical
        * luna moths use curling tails to sense
        * moths using echoing and frequencies
      * ^^mimicry^^
        * resemble other ANIMAL that’s recognized by natural enemies
        * ^^3 components^^
          * model
          * mimic
          * selective agent
        * ^^batesian mimicry^^
          * henry bates - 1933
          * mimics aposematic org but is palatable to predator
          * must be less abundant than the model
            * frequency dependent selection
          * examples
            * bee flies - mimcs bee
            * Syrphid fly - mimics bee
        * ^^mullerian mimicry^^
          * fritz muller
          * mimic aposematic model but is also unpalatable
          * both taste bad, both have advantage
          * examples
            * paperwasps - mimcs bees
            * monarch and viceroy and queen
              * monarch is model
              * viceroy is mullerian, queen is batesian

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