2.1-10-11 // Cell Structure and Function and Cell Compartmentalization

Structure determines function.

[[All cells:[[

  • Are bound by plasma membrane
  • Contains cytosol
  • Contains genetic information
  • Contains ribosomes

[[Prokaryotes[[

  • Single celled organisms
  • Domains bacteria and archaea
  • DNA is in nucleoid region (in cytosol, free floating)
  • Generally smaller

[[Eukaryotes[[

  • Multicellular organisms
  • Protists, fungi, animals, plants
  • DNA is contained within nucleus

Two classifications: ^^Endomembrane Organelles^^ and ^^Energy Organelles^^


^^Compartmentalization^^ in organelles allows for different metabolic reactions to occur in different places (giving them their own membranes to function).

  • Increases surface area
  • Prevents reactions from interfering with each other.
  • A lot of complex reactions are going on at the same time, so this allows for efficiency and to manage specific conditions necessary for certain reactions.
    • Without this, everything would be occurring in the same place at the same time and it would be chaotic.

[[Unique cell components[[

Plants:

  • ^^Chloroplasts^^
  • ^^Central vacuole^^
    • Animals have vacuoles too, but the central vacuole helps to maintain water balance and rigidity in a plant
  • ^^Cell wall^^
  • ^^Plasmodesmata^^

Animals:

  • ^^Lysosomes^^
  • ^^Centrosomes^^
  • ^^Flagella^^
    • sperms

[[Endomembrane Organelles[[

Note: ^^Metabolism^^ means to break things down.

^^Nucleus^^: Contains chromosomes (genetic info)

  • Enclosed by ^^nuclear envelope^^
  • Double membrane
    • Contains pores
  • Regulates entry and exit of materials from the cell

^^Nucleolus^^: Dense region of the nucleus where ribosomal RNA (rRNA) is synthesized

  • Combined with proteins to form large/small units of ribosomes

  • Subunits exit via nuclear pores to be assembled

  • Ribosomes translate messages found in mRNA

    Nucleus, with nucleolus in the middle

^^Ribosomes^^: Synthesizes proteins

  • Composed of rRNA and protein
  • Found in cytosol and/or bound to ER or nuclear envelope
    • Proteins produced here generally function within the cytosol (enzymes), free floating
  • Proteins produced in the ER/Nuclear envelope are secreted from the ribosomes
  • Rough ER is the one w/ ribosomes attached

Endoplasmic Reticulum: Network of membranous sacs and tubes; has membranes to help compartmentalize the cell

  • Dots = rough ER (ribosomes), no dots = smooth ER
    • On Rough ER, the ribosomes make the proteins and the ER will transfer them out.
    • Smooth ER contains no ribosomes and instead synthesizes lipids.

Golgi body: Contains flattened membranous sacs called cisternae (are not connected) and vesicles

  • Separates sacs from cytosol
  • Has directionality
    • Cis face
    • Receives proteins from ER
    • Trans face
    • Sends proteins back out into the cytosol post-modification to other locations or back to the membrane

Function: Sorts and sends out proteins to be transported to the lysosomes, plasma membrane, or to be secreted

Lysosome: Membranous sac with hydrolytic enzymes

Function:

  • Hydrolyzes macromolecules in animal cells
  • Authophagy: Lysosomes can recycle their own cell’s organic materials
    • Allows the cell to renew itself
    • EG: Breaking up nucleic acids into nucleotides to be reused

Peroxisomes: Similar to lysosomes, more specific class

  • Membrane bound metabolic compartments

Function:

  • Catalyzes reactions that produce hydrogen peroxide (H₂O₂)
    • Peroxisome (peroxide).

Vacuoles: Large vesicles; Pockets used for organizing cellular substances and creating reaction chambers

  • Stem from ER and Golgi, more selective in transport
  • Multipurpose

Types:

  • Food vacuole
    • Formed via cell eating (phagocytosis) and then are digested by lysosomes
  • Contractile vacuole
    • Maintains water levels
  • Central vacuole
    • Only in plants

Energy organelles

Mitochondrion: Site of cellular respiration

Structure of double membrane:

  • Outer is smooth
  • Inner has folds called cristae
    • Divides mitochondria into two internal compartments and increases surface area
    • Intermembrane: Space between inner/outer
    • Mitochondrial matrix: Enclosed in inner membrane
    • Location where Krebs cycle occurs
      • Has enzymes that catalyze ATP
    • Contains mitochondrial DNA and ribosomes
  • More mitochondria = more metabolic activity
    • EG: Cells that move/contract; have to do a lot of work, needs more ATP (muscles)

Chloroplasts: Specialized organelles in photosynthetic organisms

  • Performs photosynthesis
  • Contains chlorophyll (green)
  • Double membrane has:
    • Thylakoids
    • Membranous sacs that can organize into stacks called grana
    • Light dependent reactions
    • Stroma
    • Fluid around thylakoids
    • Location for Calvin cycle.
  • Contains:
    • DNA
    • Ribosomes
    • Enzymes

*both organelles have the same building blocks

Misc.

Cytoskeleton: Network of fibers throughout cytoplasm

  • Gives structural support, especially in animal cells
  • Anchors organelles
  • Not found in all cells

Endosymbiosis Theory:

  • A prokaryote enveloped a eukaryote and instead of consuming it, it remained inside of itself.

  • The prokaryotic cell would provide food molecules and energy.

  • The eukaryotic cell would provide oxygen and a safe region for the eukaryotic cell to exist.

  • Prokaryotic cell eventually lost independent function and evolved into the mitochondria/chloroplasts.

  • They became one functional organism

Evidence: (boils down to the similarities between the types of cells)

  • Both mitochondria and chloroplasts have double membranes
  • Both have ribosomes
  • Circular DNA
  • Can function on their own
  • Similar reproduction methods