Consensus Sequences associated with Alternate Sigma Subunits of E

Sigma 28 → -35 =TAAA, -10 =GCCGATAA

Sigma 32 → -35 = CTTGAA, -10 = CCCCATTA

Sigma 54 → -35 = CTTGPyAPyPu, -10 = TTGCA

Sigma 70 → -35 = TTGACA, -10 = TATAAT

What are the two most commonly occurring consensus sequences in Bacteria? Where in the promotor region are they located?

Pribnow box (TATAAT), -10

Consensus sequence (TTGACA), -35

How many polypeptides does pentameric RNA Polymerase have?

Five → alpha I/II (36.5 kD), Beta(151 kD)/ Beta’ (155 kD), omega (4 kD). Total length = 390 kD