MV

cell structures part 1

Archaean Cells

  • The lineage called Archaea was discovered less than 50 years ago.

  • Remains poorly understood, with major new groups being described regularly.

  • All archaea described to date are single-celled and relatively small compared to eukaryotes.

  • Most archaea inhabit extreme environments:

    • Deep sea

    • Hot springs

    • Frozen areas

    • Areas lacking oxygen

    • Highly basic, acidic, or salty conditions.

  • The study of archaea is challenging due to:

    • Difficulty in replicating their extreme habitats in laboratory settings.

    • Lack of known archaea causing disease in humans or having economic importance, reducing research motivation.

  • Two distinctive aspects of archaea cell structure:

    1. Unique hydrocarbon chains in membrane lipids.

    2. Unique types of carbohydrates in cell walls.

  • Connection between unique membranes/walls and habitat:

    • Their unique membranes and walls help archaea cope with extreme physical challenges (e.g., temperature, pressure, pH).

Introduction to Cells

  • The cell is the fundamental unit of life; it is the smallest entity that functions as an independent organism.

  • Individual cells are the building blocks of larger multicellular organisms.

  • Key points before exploring cell structures:

    1. Most species on Earth are single-celled organisms, found in diverse habitats:

    • Inside glacial ice.

    • In seawater at over 100°C, 3000 meters deep.

    • In rock crevices up to 5 km deep and 10 km below the ocean floor.

    • Floating in the atmosphere 11 km above Earth.

    • Within and on multicellular organisms' bodies.

    1. Three main lineages (or domains) of life (tree of life):

    • Archaea (ar-KEE-ya)

    • Bacteria

    • Eukarya (you-CARE-ya)

      • Distinctive and functionally important structural differences among these lineages.

Bacterial Cells

  • Like archaea, most bacterial cells are smaller than the average eukaryotic cell.

  • Most bacteria possess:

    • Cell walls

    • One or more flagella

  • Differences between bacteria and archaea:

    • Bacteria discovered over 450 years ago; studied due to their impact on human health and significance in commercial applications (food/drugs).

    • A few species of bacteria are multicellular, unlike archaea.

    • Bacteria have organelles and cytoskeletal structures, while evidence of these in archaea is emerging.

  • Bacterial chromosome characteristics:

    • Most bacteria have a single, circular chromosome.

    • This chromosome is viewed as an organelle due to its circular form, association with proteins, and compact structure.

Shared Cell Structures

  • Four structures found in every cell:

    1. Cell membrane: A lipid bilayer with membrane proteins and carbohydrates, regulates entry/exit of molecules.

    2. Genetic material: Heritable information in the form of DNA molecules (chromosomes), can be linear or circular.

    3. Ribosomes: Complexes of RNA and proteins; primary site for protein synthesis.

    4. Cytoskeletal elements: Provide structural support and assist in material transportation within the cell.

  • Three additional common elements:

    1. Organelles: Membrane-bound internal compartments performing specialized tasks.

    2. Cell wall: Provides rigidity and protection, usually composed of carbohydrates.

    3. Flagella: Long projections used for propulsion through water.

Size Comparison of Prokaryotic and Eukaryotic Cells

  • An average eukaryotic cell is 15,000 times larger than an average prokaryotic cell.

  • The number of average-sized prokaryotic cells that could fit inside a single average-sized eukaryotic cell would depend on cell dimensions, not volumes.

  • An example of antibiotic function shows the importance of ribosome structure:

    • Some antibiotics inhibit bacterial ribosomes, ceasing protein production and killing the bacteria.

Prokaryotic vs Eukaryotic Cells

  • Prokaryotes include archaea and bacteria (cells lacking a nucleus); eukaryotes possess a nucleus.

  • Key observations about shared structures:

    • All cells have key features like a cell membrane, chromosomes, ribosomes, and cytoskeletal elements.

    • Structure of shared components varies by lineage.

    • Components unique to each lineage include lipids in membranes and carbohydrates in cell walls, as well as distinct flagella structures.

  • Overview of organelles:

    • Organelle structures vary between prokaryotes and eukaryotes, with no organelles shared between the two.

    • Diversity and number of organelles in eukaryotes exceed those in bacteria.

  • Eukaryotic cells tend to be larger than prokaryotic cells due to efficiency in size and function.

Definitions

  • Chromosome: A DNA molecule, circular or linear depending on the species, typically associated with specific proteins.

  • Ribosome: A molecular machine of RNA and proteins, site of protein synthesis.

  • Organelle: Any membrane-bound compartment within a cell.

  • Cytoskeleton: Rod-like proteins providing structural support and facilitating transport and movement.

  • Cell wall: A rigid exterior composed of carbohydrates and other molecules, outside the cell membrane.

  • Flagellum: A long, flexible structure that propels the cell through fluid.

  • Nucleus: A double-membrane organelle containing linear chromosomes in eukaryotes.

  • Mitochondrion: An organelle in eukaryotes that produces energy by oxidizing sugars.

  • Eukaryote: Organisms whose cells contain a nucleus and mitochondria.

  • Prokaryote: Organisms in the Archaea and Bacteria lineages lacking a nucleus.

DNA and Nucleus Size Comparison

  • The total DNA in a human cell stretches up to 2 meters, while an average nucleus is just 10 micrometers (10 μm or 10 × 10^-6 m) long.

  • To compact DNA to fit in the nucleus:

    • Compaction factor = total DNA length / nucleus length, yielding 200,000 times compaction.

Unique Eukaryotic Organelle Structure

  • Mitochondrion: Surrounded by two membranes where the inner membrane forms tubes and sacs. Essential for ATP production during cellular metabolism.

  • Eukaryotic cells contain numerous membrane-bound organelles, with nucleus and mitochondrion being highly prevalent across the domain.