KJ

Microbiology: Eukaryotic Microorganisms

Eukaryotic Microorganisms: An Overview

Introduction to Microscopic Eukaryotes

  • Informal Groups: Microscopic eukaryotes are commonly categorized into key informal groups:

    • Fungi: Heterotrophic organisms characterized by chitin in their cell walls.

    • Algae: Simple autotrophs, meaning they are photosynthesizers.

    • Protozoa: Microscopic heterotrophs that are distinct from fungi.

  • Kingdom Protista: This kingdom encompasses two sub-kingdoms, primarily Algae and Protozoa.

  • Cellularity: Microscopic eukaryotes exhibit varied cellularity:

    • Always Unicellular: Protozoa.

    • May be Unicellular or Multicellular: Fungi, Algae.

    • Always Multicellular: Helminths (which are macroscopic but have microscopic unicellular egg or larval forms relevant to microbiology).

Distinguishing Eukaryotes from Prokaryotes

Eukaryotic microorganisms possess several key features that differentiate them from prokaryotes:

  • Presence of a true nucleus encapsulating genetic material.

  • Existence of various membrane-bound organelles (e.g., mitochondria, endoplasmic reticulum).

  • They may exist in haploid and/or diploid states.

  • Asexual reproduction primarily occurs via mitosis.

  • Sexual reproduction is accomplished through meiosis.

Kingdom Fungi

Mycology and Fungal Forms
  • Mycology: The scientific study of fungi.

  • Microscopic Fungi: These include yeasts and molds, which are morphological designations rather than taxonomic classifications.

    • Yeasts: Unicellular fungi.

    • Molds: Filamentous fungi.

Structure of Fungi
  • Yeasts: Are strictly unicellular; individual cells separate after cell division.

  • Filamentous Fungi (Molds): Most microscopic fungi have a filamentous structure, composed of individual threads called hyphae.

  • Mycelium: A visible mass of hyphae forms a mycelium.

  • Types of Hyphae:

    • Submerged (vegetative) hyphae: Involved in nutrient absorption.

    • Aerial (reproductive) hyphae: Extend into the air and produce spores.

  • Dimorphic Fungi: These fungi have the unique ability to grow as either single yeast cells or multicellular mycelia, depending on environmental conditions.

    • Examples: Candida albicans, Histoplasma capsulatum.

Fungal Physiology and Ecological Role
  • Cell Walls: Primarily composed of chitin.

  • Cell Membranes: Typically contain ergosterol.

  • Exoenzymes: Fungi secrete powerful exoenzymes outside their cells to break down larger polymeric molecules like polysaccharides and proteins into smaller, absorbable units.

  • Decomposition: Alongside bacteria, fungi are the principal decomposers in ecosystems, capable of degrading tough substances like cellulose and lignin (found in wood).

  • Nutrient Cycling: Their activity releases carbon dioxide (CO_2) and various nitrogen compounds back into the soil, crucial for nutrient cycling.

  • Saprobes: Most fungi are saprobes, meaning they obtain nutrients primarily from dead or decaying organic matter.

  • Parasitism: Some fungi act as parasites of living tissues, though none are obligate parasites.

  • Infections: Few fungal species infect humans; however, plant infections caused by fungi are exceedingly common and economically significant.

Reproduction in Fungi
  • Yeast Reproduction: Reproduce primarily by mitosis or through a process called budding.

  • Mold Reproduction: Produce hyphae that can be classified as:

    • Septate: Possessing cross-walls.

    • Non-septate (coenocytic): Lacking cross-walls, appearing as a continuous multinucleated tube.

  • Spore Production: Aerial hyphae produce new spores.

    • Asexual Spores: Produced via mitosis.

    • Sexual Spores: Produced via meiosis.

  • Sexual Reproduction: Sexual spores combine to form zygotes, a process that is often used as a basis for fungal categorization.

Classification of Fungi
  • The classification of fungi (Eumycota) is currently undergoing updates and is often described as being