Hypertonic, Hypotonic, and Isotonic Solutions
Solutions and Their Effects on Cells
Introduction to Solutions and Cells
Example: Three containers with saltwater solutions and a giant cell placed in each.
Importance of understanding tonicity: hypertonic, hypotonic, isotonic.
Identifying Tonicity
Isotonic Solution
Middle container is isotonic.
Definition: Solute concentration outside the cell equals solute concentration inside the cell.
Hypertonic Solution
Left container is hypertonic.
Definition: Solute concentration outside the cell is greater than inside the cell.
The "hyper" prefix signifies higher.
Hypotonic Solution
Right container is hypotonic.
Definition: Solute concentration outside the cell is less than inside the cell.
The "hypo" prefix signifies lower.
Concentration and Water Movement
Relationship between solute concentration and water concentration:
High saline concentration = low water concentration.
Low solute concentration = high water concentration.
Cell Membrane and Osmosis
Assumed semi-permeable membrane:
Allows water movement but not ions (sodium and chloride).
Osmosis
Definition: Diffusion of water.
Movement of water: Higher to lower concentration, influenced by ion presence.
Effects of Solutions on Cells
Hypertonic Solution Effects
Water flows out of the cell because outside concentration has lower water content.
Net movement of water: Outward, leading to cell shriveling (plasmolysis).
Isotonic Solution Effects
Equal concentration of solutes inside and outside the cell.
Equal flow of water in both directions, resulting in no net movement.
Cell maintains its shape.
Hypotonic Solution Effects
Outside solution has lower solute concentration and higher water concentration.
Water flows into the cell due to higher concentration of water outside than inside.
Cell swells and may burst, resulting in cytolysis.
Analyzing Water Movement
Two perspectives on water movement:
From water concentration: High to low concentration.
From solute concentration: Water moves toward areas of high solute concentration.
Interaction of Water with Ions
Water's polarity influences attraction to ions.
Sodium ions:
Water's oxygen (partial negative charge) attracted to sodium (positive charge).
Chloride ions:
Water's hydrogen (partial positive charge) attracted to chloride (negative charge).
Conclusion
Summary of cellular response to different solutions:
Hypertonic = shriveling.
Isotonic = no change.
Hypotonic = swelling/bursting.
Importance of tonicity in biological systems.