Recombinant DNA

Recombinant DNA

Learning Goal: Appreciate how restriction enzymes allow scientists to create recombinant DNA molecules 

in the lab.

•Explain how restriction enzymes recognize and cut DNA.

-palindomatic and cut at restriction sites

•Outline the steps involved in creating a recombinant DNA plasmid.

Cutting DNA with restriction enzymes at restriction site 

• Identify potential restriction enzyme sites based on the sequence of nucleotides.

•Determine whether two different restriction enzymes would generate compatible “sticky ends” given the

recognition sequence and cut site for the enzymes.

•Determine the size(s) of restriction fragments produced when a linear or circular piece of DNA is cut by one or

more restriction enzymes.

•Compare and contrast the genetic information stored in complementary DNA (cDNA) vs.genomic DNA.

-cDNA is a synthetic DNA copy of mRNA and is therefore just the protein encoding region of DNA

While genomic DNA still contains introns