Genetics and Inheritance Patterns
Allele Variations and Phenotypes
Heterozygous Condition
A person who is heterozygous at a given locus (gene pair) exhibits a wavy hair phenotype.
Genotype and Phenotype Definitions
Homozygous: When an individual has two identical alleles at a locus.
Homozygous Dominant: Two dominant alleles (e.g., curly hair).
Phenotype: Curly hair.
Heterozygous: Two different alleles at a locus.
Phenotype: Wavy hair.
Heterozygous Recessive: When an individual carries one recessive allele.
Phenotype: Straight hair.
Dominance Hierarchy
Dominant Phenotype: Curly hair (homozygous dominant).
Recessive Phenotype: Straight hair.
Intermediate Phenotype: Wavy hair in heterozygotes indicates incomplete dominance.
Incomplete Dominance
Definition: A genetic situation where one allele does not completely dominate another, resulting in a phenotype that is a blend of both.
In heterozygotes, they produce less of a protein than homozygous dominants, leading to intermediate traits (e.g., wavy hair).
Heterozygotes for that gene produce an intermediate phenotype (wavy hair).
Codominance
Definition: A genetic scenario where two dominant alleles are expressed simultaneously.
The term "co" means together—implying both traits are seen.
Example of Codominance: Hemoglobin
Hemoglobin consists of four protein chains (globins).
Different alleles for globin chains lead to two types of hemoglobin:
Adult Hemoglobin (dominant): Normal globin, normal hemoglobin carrying oxygen effectively.
Sickle Allele: A mutation that alters one nucleotide in the instructions, producing sickle hemoglobin leading to sickle-shaped red blood cells.
Function of Hemoglobin
Hemoglobin binds oxygen in red blood cells and transports it through the blood.
When it binds to oxygen, it releases carbon dioxide, a byproduct of cellular respiration.
Sickle hemoglobin aggregates, resulting in deformation of red blood cells, which can lead to blockage in blood vessels and organ damage.
Phenotypes Associated with Hemoglobin Genetics
Normal Hemoglobin:
Genotype: Two normal alleles.
Phenotype: Normal shape and function of red blood cells.
Sickle Cell Disease (SCD):
Genotype: Two sickle alleles.
Phenotype: Produces only sickle hemoglobin, resulting in sickled red blood cells.
Sickle Cell Trait (SCT):
Genotype: One normal allele and one sickle allele.
Phenotype: Produces both normal and sickle hemoglobin, typically exhibits no major health problems but can have anatomical variations under stress.
Medical Implications of Sickle Cell Disease
Individuals may suffer from severe pain episodes (sickle cell crisis) during low oxygen availability.
Regular blood transfusions are often required due to the risks of organ damage and other complications.
Gene editing research is currently underway aiming for potential cures.
Evolutionary Advantage of Sickle Cell Trait
Sickle cell trait provides resistance to malaria, which can be more deadly than sickle cell disease in certain populations (e.g., areas with high malaria rates).
This is why the allele remains prevalent within some populations despite the severe implications of having two sickle alleles.
Polygenic Inheritance
Definition: Involves multiple genes contributing to a single trait.
Examples include height, skin pigmentation, and eye color.
Polygenic traits demonstrate continuous variation — for instance, height is influenced by many genes controlling various aspects of growth.
Example of Polygenic Trait: Height
Traits like height are determined by contributions from at least 100 genes plus environmental factors (e.g., nutrition).
Average height may vary widely across individuals in a population, indicative of polygenic inheritance.
Skin and Hair Color Genetics
Skin pigmentation is mainly controlled by the pigment melanin, produced by melanocytes.
Melanin Types:
Eumelanin: Produces brownish colors.
Pheomelanin: Related to reddish hues.
Lethal Genes
Definition: Some genotypes result in embryonic death, leading to missing phenotypes in offspring.
Example: Certain genes involved in spinal cord development affecting tail length in cats may lead to varying developmental outcomes.
Mink Cats
Result from a gene leading to shortened spinal cord development (M gene).
Heterozygous individuals might develop normally, while homozygous individuals (two copies of M) may not survive pregnancy.
Summary of Dominance Types
Complete Dominance: One allele completely masks the other (example: eye shape).
Incomplete Dominance: Blending of traits (example: hair texture).
Codominance: Expression of both alleles (example: hemoglobin).
Review and Application
Understanding these concepts is crucial for practical applications within laboratory settings, especially in genetics.
Situations that illustrate these principles may be encountered during genetic studies, making mastery of the material essential for successful outcomes in the course.
Y Chromosome and Sex Determination
Y Chromosome Overview
Contains up to 80 functional genes that influence male development.
The SRY gene (Sex-determining Region Y) acts as a master control gene.
The SRY gene is initially nonfunctional; it is turned on during a critical period of embryonic development, stimulating the production of testosterone, which leads to the development of male characteristics.
Development of male sexual characteristics occurs in two stages:
First: Early in embryo development.
Second: During puberty, when another burst of testosterone promotes secondary male characteristics.
If SRY is not activated, the fetus will develop female characteristics.
Hormonal Production in Males and Females
All humans produce both testosterone and estrogen.
The difference lies in the amount produced, not the type of hormones.
Sex Determination Systems in Various Organisms
Humans and fruit flies:
Males (XY) and females (XX) have different sex chromosomes.
Birds:
Males have the same type of sex chromosomes, and the females have different types (ZW system).
Grasshoppers:
Females have two of the same sex chromosomes (XX), while males possess only one (X).
Bees and ants:
Females develop from fertilized eggs (diploid), while males develop from unfertilized eggs (haploid).
Experiments with Fruit Flies
Background:
Early genetic experiments utilized fruit flies (Drosophila) due to their simple genetics.
Mutant fruit flies produced interesting results, including white-eyed variants (lacking red pigment).
Genotypes:
Female fruit flies can be homozygous for red eyes (dominant allele) or heterozygous (one allele for red, one for white).
Male fruit flies have only one X chromosome, leading to hemizygosity.
Results of Crossbreeding:
Crossing red-eyed females with white-eyed males produced all red-eyed offspring due to the dominant allele's expression in females.
Heterozygous females pass on red-eyed alleles to male offspring, leading to different ratios of red-eyed to white-eyed males (indicates X-linked inheritance).
Human Eye Color Genetics
Similar patterns observed in humans:
Eye color traits are often X-linked, revealing ratios in males and females.
Retina Composition:
Comprised of rod cells (light sensing) and cone cells (color sensing).
Color blindness is often tied to mutations in the cone cells.
Red-Green Color Blindness:
Condition where individuals cannot perceive red or green hues due to mutated alleles on the X chromosome.
Punnett Square for Color Blindness:
Using symbols (e.g., L for normal vision, l for colorblindness):
Mother: Heterozygous (L/l)
Father: Homozygous dominant (L/—; normal vision)
Offspring Analysis:
Both daughters can see normally.
Sons have a probability of being colorblind (one son may be colorblind if he inherits l).
Hemizygous Definition:
Term for males regarding X-linked traits since they possess one allele (e.g., hemizygous dominant or hemizygous recessive).
Female Color Blindness
A female can only be color blind if she is homozygous recessive (l/l) for the colorblind allele.
If her father is colorblind, she will inherit the recessive allele from him.
Discussion of complications in a male’s life due to color blindness (e.g., military concerns).
Nondisjunction in Meiosis
Definition:
Nondisjunction occurs when homologous chromosomes do not separate properly during meiosis, leading to gametes with abnormal chromosome numbers.
Mechanisms of Nondisjunction:
Can happen during Meiosis I (both chromosomes move to one side of the division) or Meiosis II (sister chromatids do not separate).
Consequences:
Human gametes: Normal = 23 chromosomes.
Nondisjunction can lead to gametes with 22 (missing one chromosome) or 24 (extra chromosome) chromosomes.
Age Factor in Females:
Eggs are arrested in Meiosis I at birth; nondisjunction more common after age 35 due to the aging of eggs.
Trisomy Conditions
Down Syndrome (Trisomy 21):
Caused by an extra copy of chromosome 21.
Survival rates relatively high due to smaller chromosome size and fewer critical genes.
Associated health challenges like developmental delays and organ enlargement.
Trisomy 13 and Other Autosomal Trisomies:
Trisomy 13 usually leads to non-survivable conditions shortly after birth.
Other autosomal trisomies are often fatal early in development.
Conclusion and Upcoming Topics
Next discussions will cover nondisjunction involving sex chromosomes, which tend to be more viable than those relating to autosomes.
Chromosomal Disorders
Y Chromosome
Associated disorders by chromosomal abnormalities
Turner syndrome (monosomy X)
Characterized by the presence of only one X chromosome
Klinefelter syndrome
Characterized by two X chromosomes and one Y chromosome (XXY)
Jacob's syndrome
Characterized by one X and two Y chromosomes (XYY)
Importance of distinguishing between these syndromes for proper diagnosis and management
Dosage Compensation
Definition: Dosage compensation
Mechanism to equalize gene expression between sexes with different sex chromosome numbers
X chromosome dosage in females (two Xs) is compensated by inactivating one X chromosome
Overview of X chromosome inactivation
In females, only one X chromosome remains active; the other is randomly deactivated
Incident of Gas Leak
A message was received indicating that there is a gas leak at the building.
Important Points:
The gas leak is not affecting anyone in any way.
Authorities have the situation under control.
Everyone is instructed to shelter in place to avoid exposure to gas clouds.
Communication is encouraged; notify if you receive any updates via text.
Understanding Nondisjunction
Definition:
Nondisjunction refers to an error that occurs during meiosis, where improper separation of chromosomes takes place.
Types of Nondisjunction:
Meiosis I: Homologous chromosomes do not separate properly.
Meiosis II: Sister chromatids do not separate properly.
Consequences:
Resulting gametes contain an abnormal number of chromosomes, leading to chromosomal abnormalities.
Chromosomal Abnormalities in GametesAutosomes
Important Concepts:
Autosomes are the non-sex chromosomes.
Chromosomal abnormalities result in serious developmental issues for the fetus.
Common Examples:
Trisomy 21:
The most common form of autosomal trisomy.
Characterized by having an extra copy of chromosome 21.
Relatively fewer genes on chromosome 21 due to its size.
Trisomy 13:
Involves an extra copy of chromosome 13.
Infants with this condition typically do not survive beyond a few weeks or months.
Implications:
Both excess and inadequate autosomes lead to unviable outcomes; survivability decreases with aberrations in chromosomal count.
Sex Chromosomes
Differing Mechanisms:
The effects of nondisjunction can be quite different between sex chromosomes compared to autosomes.
Sperm and Egg Chromosomal Contributions:
Cases presented include sperm with nonnormal chromosomes fertilizing a normal egg, and vice versa.
Example 1:
A sperm lacking any sex chromosome (nondisjunction) would fertilize a normal egg.
Resulting zygote:
X chromosome from the egg and no other sex chromosome causes the development of Turner Syndrome (monosomy X).
Symptoms commonly include developmental delays in early development stages.
Shift in X chromosome activation: Extra X chromosomes in females typically become deactivated at a certain developmental stage.
Example 2:
A normal sperm fertilizing an egg with irregular sex chromosomes (not detailed, indicating reverse nondisjunction cases).
Effects would depend on the type of irregularity in the egg's sex chromosome count.