Cell Death and Necrosis Mechanisms

Overview of Cellular Responses to Stress

  • Focus on cellular responses to stress, leading to adaptations or injury.

Stress and Cell Response

  • Stress can lead to growth adaptations:

    • Hyperplasia: Increase in the number of cells.

    • Hypertrophy: Increase in cell size.

  • Reducing stress can lead to atrophy: Decrease in cell size or function.

  • A change in stress level can lead to anaplasia: Reversion to a more primitive cell type.

Cellular Injury and Death

  • Severe stress results in injury:

    • Initial stage is known as reversible cellular injury.

    • Persistent injury leads to irreversible injury, also termed cell death.

Hallmarks of Cell Death

  • Morphologic hallmark of cell death:

    • Loss of nucleus (if nuclei are absent, tissue is considered dead).

    • Stages of nuclear loss:

    • Pyknosis: Shrinking of the nucleus.

    • Karyorrhexis: Fragmentation of the nucleus.

    • Karyolysis: Dissolution of the nucleus into basic components.

Mechanisms of Cell Death

  • Two primary mechanisms:

    1. Necrosis

    • Described as murder; involves external pathological processes leading to large-scale tissue destruction.

    • Always leads to acute inflammation.

    • Necrosis is not a physiological process; it indicates disease or serious injury.

    • Types of necrosis, often examined:

      • Coagulative necrosis:

        • Tissue remains firm with retained architecture despite cell death.

        • Hallmark characteristics: Lost nuclei, firm necrotic tissue.

        • Typically occurs in ischemic infarction (like myocardial infarction).

        • Wedge-shaped, pale areas of infarction based on blood supply patterns.

        • Liquefaction necrosis:

        • Necrotic tissue becomes liquefied and may be seen in diabetic patients.

        • Often associated with infections leading to pus formation.

        • Caseous necrosis:

        • A specific type of liquefaction necrosis associated with tuberculosis or fungal infections, leading to cottage cheese-like appearance.

        • Fat necrosis:

        • Involves necrosis of adipose tissue, appearing chalky white due to fat release.

        • Common in acute pancreatitis due to fat cell breakdown.

        • Trauma to breast can also lead to fat necrosis, potentially presenting as a mass with giant cell reactivity.

        • Subconfication: Calcium binding with damaged fatty acids, also relates to the soap-making process and is a form of dystrophic calcification.

Summary Points About Necrosis

  • Dystrophic calcification: Calcium deposits in necrotic or dying tissues despite normal serum levels.

  • Metastatic calcification: Involves abnormal calcium metabolism, leading to deposits irrespective of local tissue necrosis.

  • Important to note that examinations may inquire about both the characteristics of necrosis types and their physiological implications.